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This paper compares the efficacy of two post\u2010mortem sperm retrieval techniques \u2010 the flushing and float\u2010out methods \u2010 in the collection of rooster sperm, in conjunction with the use of two extenders, i.e., L&amp;R\u201084 medium and Lake 7.1 medium. To determine whether the protective effects of these extenders against refrigeration are different for post\u2010mortem and ejaculated sperm, pooled ejaculated samples (procured via the massage technique) were also diluted in the above extenders. Post\u2010mortem and ejaculated sperm variables were assessed immediately at room temperature (0\u00a0h), and after refrigeration at 5\u00b0C for 24 and 48\u00a0h. The flushing method retrieved more sperm than the float\u2010out method (596.5\u00a0\u00b1\u00a075.4\u00a0million sperm vs 341.0\u00a0\u00b1\u00a087.6\u00a0million sperm; p\u00a0&lt;\u00a00.05); indeed, the number retrieved by the former method was similar to that obtained by massage\u2010induced ejaculation (630.3\u00a0\u00b1\u00a078.2\u00a0million sperm). For sperm collected by all methods, the L&amp;R\u201084 medium provided an advantage in terms of sperm motility variables at 0\u00a0h. In the refrigerated sperm samples, however, the Lake 7.1 medium was associated with higher percentages of viable sperm, and had a greater protective effect (p\u00a0&lt;\u00a00.05) with respect to most motility variables. In conclusion, the flushing method is recommended for collecting sperm from dead birds. If this sperm needs to be refrigerated at 5\u00b0C until analysis, Lake 7.1 medium is recommended as an extender.<\/jats:p>","DOI":"10.1111\/rda.12643","type":"journal-article","created":{"date-parts":[[2015,11,25]],"date-time":"2015-11-25T06:56:50Z","timestamp":1448434610000},"page":"40-46","source":"Crossref","is-referenced-by-count":7,"title":["Influence of Post\u2010Mortem Sperm Recovery Method and Extender on Unstored and Refrigerated Rooster Sperm Variables"],"prefix":"10.1111","volume":"51","author":[{"given":"S","family":"Villaverde\u2010Morcillo","sequence":"first","affiliation":[{"name":"Veterinary Clinical and Research Services Fieb Foundation Tres Cantos Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MC","family":"Esteso","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction INIA Madrid 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