{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T17:04:32Z","timestamp":1732035872424},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,12,23]],"date-time":"2015-12-23T00:00:00Z","timestamp":1450828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006668","name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006668","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The mechanisms involved in fertilization are the centre of attention in order to determine the conditions required to reproduce <jats:italic>in\u00a0vitro<\/jats:italic> the events that take place <jats:italic>in\u00a0vivo<\/jats:italic>, with special interest in endangered species. Previous data from mouse sperm, where acrosome reaction (<jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content>) occurs more often in the interstitium of the cumulus oophorus, contribute to strengthen the use of progesterone as a physiological inducer of this process. We studied the participation of protein kinase A (<jats:styled-content style=\"fixed-case\">PKA<\/jats:styled-content>), phospholipases A<jats:sub>2<\/jats:sub> and C (<jats:styled-content style=\"fixed-case\">PLA<\/jats:styled-content><jats:sub>2<\/jats:sub>, <jats:styled-content style=\"fixed-case\">PLC<\/jats:styled-content>) in the <jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> induced by progesterone from <jats:italic>Chinchilla<\/jats:italic> epididymal spermatozoa. The addition of db\u2010<jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content> to the incubation medium caused an increase of 58% in the <jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content>, while the use of H89 (30\u00a0<jats:italic>\u03bc<\/jats:italic><jats:sc>m<\/jats:sc>), a <jats:styled-content style=\"fixed-case\">PKA<\/jats:styled-content> inhibitor, reflected a decrease of 40% in the percentage of reacted gametes. The assays conducted with arachidonic acid showed a maximum increase of 23% in the <jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content>. When gametes were pre\u2010incubated with <jats:styled-content style=\"fixed-case\">PLA<\/jats:styled-content><jats:sub>2<\/jats:sub> inhibitors, a dose\u2010dependent inhibitory effect was observed. The addition of phorbol12\u2010myristate13\u2010acetate (10\u00a0<jats:italic>\u03bc<\/jats:italic><jats:sc>m<\/jats:sc>) revealed higher percentages of <jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> induction (60%). When <jats:styled-content style=\"fixed-case\">PLC<\/jats:styled-content> was inhibited with neomycin and U73122, a dose\u2010dependent decrease in <jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content> percentages was observed. Combined inhibition of <jats:styled-content style=\"fixed-case\">PKA<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> PLA<\/jats:styled-content><jats:sub>2<\/jats:sub> and <jats:styled-content style=\"fixed-case\">PLC<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> AR<\/jats:styled-content> values similar to control were obtained. This work shows evidence, for the first time in Chinchilla, that progesterone activates the <jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">cAMP<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">PKA<\/jats:styled-content> system as well as sperm phospholipases and that these signalling pathways participate jointly and cooperatively in <jats:styled-content style=\"fixed-case\">AR<\/jats:styled-content>. These results contribute to the understanding of the complex regulation that is triggered in sperm after the effect of progesterone.<\/jats:p>","DOI":"10.1111\/rda.12659","type":"journal-article","created":{"date-parts":[[2015,12,24]],"date-time":"2015-12-24T03:58:42Z","timestamp":1450929522000},"page":"150-157","source":"Crossref","is-referenced-by-count":3,"title":["Involvement of Protein <scp>cAMP<\/scp>\u2010dependent Kinase, Phospholipase A<sub>2<\/sub> and Phospholipase C in Sperm Acrosome Reaction of <i>Chinchilla lanigera<\/i>"],"prefix":"10.1111","volume":"51","author":[{"given":"MC","family":"Gramajo\u2010B\u00fchler","sequence":"first","affiliation":[{"name":"Department of Developmental Biology Higher Institute for Biological Research San Miguel de Tucum\u00e1n Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Zelaray\u00e1n","sequence":"additional","affiliation":[{"name":"Faculty of biochemistry, chemistry and Pharmacy Institute of 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