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We used epididymal sperm of the domestic cat to optimize a sperm freezing procedure for felid species, particularly questioning the demand for sperm cooling to 4\u00b0C. We equilibrated sperm during slow cooling to only 15 or 10\u00b0C in a Tes\u2013Tris\u2013fructose extender with final concentrations of 4.7% (v\/v) glycerol and 10% (v\/v) of the water\u2010soluble fraction of hen's egg yolk (low\u2010density lipoproteins). Subsequently, sperm were frozen over liquid nitrogen. Total and progressive motility (mean\u00a0\u00b1\u00a0<jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content>) after thawing was 60.7\u00a0\u00b1\u00a08.6% and 53.9\u00a0\u00b1\u00a09.6% in samples cooled to 15\u00b0C or 61.6\u00a0\u00b1\u00a09.5% and 55.3\u00a0\u00b1\u00a09.9% in samples cooled to 10\u00b0C. Therefore, a one\u2010step addition of glycerol to sperm at room temperature together with the freezing extender, the use of cryovials (loaded with diluted sperm aliquots of 300\u00a0\u03bcl), an equilibration period of 40\u00a0min comprising slow cooling to 15\u00b0C at a rate of approximately \u22120.14\u00a0K\/min before rapid freezing over liquid nitrogen, yielded satisfying results. Cooling, freezing and thawing rates were exactly characterized as a prerequisite for further optimization and to provide a repeatable protocol to other practitioners.<\/jats:p>","DOI":"10.1111\/rda.12666","type":"journal-article","created":{"date-parts":[[2016,1,28]],"date-time":"2016-01-28T08:27:43Z","timestamp":1453969663000},"page":"195-203","source":"Crossref","is-referenced-by-count":18,"title":["Successful Cryopreservation of Domestic Cat (<i><scp>F<\/scp>elis catus<\/i>) Epididymal Sperm after Slow Equilibration to 15 or 10\u00b0C"],"prefix":"10.1111","volume":"51","author":[{"given":"C","family":"Klaus","sequence":"first","affiliation":[{"name":"Leibniz\u2010Institut f\u00fcr Zoo\u2010 und Wildtierforschung Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Eder","sequence":"additional","affiliation":[{"name":"Leibniz\u2010Institut f\u00fcr Zoo\u2010 und Wildtierforschung Berlin 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