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Ejaculates were obtained from 31 stud dogs, and the sperm\u2010rich fraction (<jats:styled-content style=\"fixed-case\">SRF<\/jats:styled-content>) was kept for analysis. One aliquot was used for the analysis of concentration, progressive motility (P; <jats:styled-content style=\"fixed-case\">CASA<\/jats:styled-content>), viability (V; <jats:styled-content style=\"fixed-case\">CASA<\/jats:styled-content>) and leucocyte count, and the analysis was performed by flow cytometry (<jats:styled-content style=\"fixed-case\">FITC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PNA<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">SCSA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HOST<\/jats:styled-content>. In seminal plasma, concentration of albumin, cholesterol, calcium, inorganic phosphate, sodium, potassium, zinc and copper was measured. Semen smears were prepared and evaluated for the expression of <jats:styled-content style=\"fixed-case\">ABCA<\/jats:styled-content>1. The remainder of each ejaculate was frozen. After thawing, the quality assessment was repeated and further smears were prepared. According to post\u2010thaw semen quality, dogs were assigned to good freezers (n\u00a0=\u00a020) or bad freezers (n\u00a0=\u00a011), the latter were defined as &lt;\u00a050% progressive motility and\/or &gt;\u00a040% morphologically abnormal sperm and\/or &lt;\u00a050% viability. Bad freezers were older than good freezers (5.3 vs 3.4\u00a0years, p\u00a0&lt;\u00a00.05). In bad freezers, the percentage of sperm with <jats:styled-content style=\"fixed-case\">ABCA<\/jats:styled-content>1 signal in the acrosome was lower (26.3% vs 35.7%, p\u00a0&lt;\u00a00.01) and the percentage of sperm with complete loss of <jats:styled-content style=\"fixed-case\">ABCA<\/jats:styled-content>1 signal higher (46.7% vs 30%, p\u00a0&lt;\u00a00.01); the percentage of dead spermatozoa was higher (36.1% vs 25.5%, p\u00a0&lt;\u00a00.05), and the concentration of cholesterol and sodium in seminal plasma was lower than in good freezers (p\u00a0&lt;\u00a00.05). We conclude that in thawed bad freezer sperm, an increase in acrosome damages coincided with an increased loss of cholesterol transporters and cell death, and a lower cholesterol concentration in seminal plasma. Follow\u2010up studies revealed whether a relation exists between these findings.<\/jats:p>","DOI":"10.1111\/rda.12671","type":"journal-article","created":{"date-parts":[[2016,2,5]],"date-time":"2016-02-05T05:59:29Z","timestamp":1454651969000},"page":"232-239","source":"Crossref","is-referenced-by-count":15,"title":["Seminal Plasma Characteristics and Expression of <scp>ATP<\/scp>\u2010binding Cassette Transporter A1 (<scp>ABCA<\/scp>1) in Canine Spermatozoa from Ejaculates with Good and Bad Freezability"],"prefix":"10.1111","volume":"51","author":[{"given":"S","family":"Sch\u00e4fer\u2010Somi","sequence":"first","affiliation":[{"name":"Platform for Artificial Insemination and Embryo Transfer Vetmeduni Vienna Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Palme","sequence":"additional","affiliation":[{"name":"Platform for Artificial Insemination and Embryo Transfer Vetmeduni Vienna Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,2,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2009.04.012"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2010.01.009"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2006.03.018"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(03)00174-2"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2008.11.007"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0011-2240(90)90009-S"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3923\/pjbs.2008.1964.1968"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.05.017"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2009.01417.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009844109023"},{"issue":"2","key":"e_1_2_8_12_1","first-page":"1","article-title":"Factors influencing the quantity and quality of semen harvested from bulls, rams, boars and stallions","volume":"47","author":"Foote RH","year":"1978","journal-title":"J Anim Sci"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01203826"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402300316"},{"key":"e_1_2_8_15_1","first-page":"287","volume-title":"Canine and Feline Theriogenology","author":"Johnston S","year":"2001"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.09.011"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.07.015"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2015.07.014"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(93)90230-3"},{"key":"e_1_2_8_20_1","unstructured":"Linde\u2010ForsbergC.Document No. A1209.0501. 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