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Fresh embryos, and embryos cryopreserved using a standardized slow freezing procedure (direct thaw\/direct transfer, <jats:styled-content style=\"fixed-case\">DT<\/jats:styled-content>) served as <jats:styled-content style=\"fixed-case\">ET<\/jats:styled-content> controls. Two different follicle\u2010stimulating hormone (<jats:styled-content style=\"fixed-case\">FSH<\/jats:styled-content>) sources, Folltropin<jats:sup>\u00ae<\/jats:sup> Canada (<jats:styled-content style=\"fixed-case\">FSH BAH<\/jats:styled-content>, 24 donors) and a brand prepared by the Chinese Academy of Science (<jats:styled-content style=\"fixed-case\">FSH CAS<\/jats:styled-content>, 16 donors), were compared in a series of superovulation outcomes following well\u2010established <jats:styled-content style=\"fixed-case\">FSH<\/jats:styled-content> administration protocols. Following data analysis, the total number of ovulations recorded at the time of embryo flushing (10.5 vs 8.5; p\u00a0=\u00a00.28) and the total number of transferable embryos (6.2 vs 5.1; p\u00a0=\u00a00.52) were similar between the two <jats:styled-content style=\"fixed-case\">FSH<\/jats:styled-content> sources. <jats:styled-content style=\"fixed-case\">ET<\/jats:styled-content> for <jats:styled-content style=\"fixed-case\">MDV<\/jats:styled-content> (39.7%, n\u00a0=\u00a078), <jats:styled-content style=\"fixed-case\">DT<\/jats:styled-content> (35.2%, n\u00a0=\u00a071) and fresh controls (47.1%, n\u00a0=\u00a034) resulted in similar pregnancy rates (p\u00a0&gt;\u00a00.05). When <jats:styled-content style=\"fixed-case\">MDV<\/jats:styled-content> was used, a higher pregnancy rate (42.6%) resulted from the transfer of vitrified morulae, when compared to the <jats:styled-content style=\"fixed-case\">DT<\/jats:styled-content> counterparts (24.3%), (p\u00a0=\u00a00.05). Transfer of vitrified morulae resulted also in higher pregnancy rate, when compared to the transfer of vitrified blastocysts (42.6% vs. 29.4%; p\u00a0&lt;\u00a00.05). Transfer of <jats:styled-content style=\"fixed-case\">DT<\/jats:styled-content> blastocysts resulted in higher pregnancy rate than morulae, similarly cryopreserved (47.1% vs. 24.3%, p\u00a0&lt;\u00a00.05). In conclusion, <jats:styled-content style=\"fixed-case\">MDV<\/jats:styled-content> is an effective alternative methodology for cryopreservation of <jats:italic>in\u00a0vivo<\/jats:italic>\u2010derived embryos. This study gives also indication that, compared to vitrified blastocysts, <jats:styled-content style=\"fixed-case\">MDV<\/jats:styled-content> of morula stage embryos results in higher pregnancy rates following warming and transfer into synchronized recipients.<\/jats:p>","DOI":"10.1111\/rda.12674","type":"journal-article","created":{"date-parts":[[2016,2,8]],"date-time":"2016-02-08T06:35:23Z","timestamp":1454913323000},"page":"255-261","source":"Crossref","is-referenced-by-count":12,"title":["Successful Vitrification of <i>In vivo<\/i> Embryos Collected from Superovulated Japanese Black Cattle (Wagyu)"],"prefix":"10.1111","volume":"51","author":[{"given":"L","family":"An","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory for Molecular and Medical Biotechnology College of Life Sciences Nanjing Normal University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PP","family":"Ling","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Molecular and Medical Biotechnology College of Life Sciences Nanjing Normal University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X","family":"Zhu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Molecular and Medical Biotechnology College of Life Sciences Nanjing Normal University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Molecular and Medical Biotechnology College of Life Sciences Nanjing Normal University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Molecular and Medical Biotechnology College of Life Sciences Nanjing Normal University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X","family":"Ma","sequence":"additional","affiliation":[{"name":"Lannuo Biotechnologies Wuxi Inc. 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