{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T11:11:54Z","timestamp":1783941114923,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,5,8]],"date-time":"2016-05-08T00:00:00Z","timestamp":1462665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"China Agriculture Research System","award":["CARS-39-03A"],"award-info":[{"award-number":["CARS-39-03A"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Relatively molecular mass of Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content> antigens is small and hence needs to couple to a large carrier molecule to enhance its immunogenicity. This study investigated whether hepatitis B surface antigen S (<jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content>sAg\u2010S) gene can be used as an effective carrier molecule for developing Gn<jats:styled-content style=\"fixed-case\">RH DNA<\/jats:styled-content> immunocastration vaccine. Two copies of human Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content> gene were fused with <jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content>sAg\u2010S gene for constructing a recombinant plasmid <jats:styled-content style=\"fixed-case\">pVAX<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content>sAg\u2010S\u20102Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content> that coded for 27\u00a0<jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>a target fusion protein. Ten male mice were divided into two equal groups, treatment and control. The vaccine (50\u00a0\u03bcg\/mice) prepared in saline solution was injected into male mice at weeks 0, 1, 2, 4 and 7 of the experiment. Vaccine's efficacy was evaluated in terms of Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>\u2010specific IgG antibody response, plasma testosterone levels, testicular weight and extent of the testicular tissue damage. The specific anti\u2010Gn<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content> antibody titre in vaccinated animals was significantly higher than in controls in only 4th week of immunization (p\u00a0<jats:italic>&lt;<\/jats:italic>\u00a00.05). In addition, vaccinated animals showed lower testicular weight than those of the controls (p\u00a0&lt;\u00a00.05). Spermatogenesis in seminiferous tubules in vaccinated animals was suppressed. In conclusion, in this study, the engineered plasmid to be used as a Gn<jats:styled-content style=\"fixed-case\">RH DNA<\/jats:styled-content> vaccine induced antibody response and suppressed spermatogenesis in mice. This suggests that <jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content>sAg\u2010S gene can be an effective carrier molecule for developing Gn<jats:styled-content style=\"fixed-case\">RH DNA<\/jats:styled-content> immunocastration vaccine when relatively molecular mass of the aimed antigens is small.<\/jats:p>","DOI":"10.1111\/rda.12692","type":"journal-article","created":{"date-parts":[[2016,5,9]],"date-time":"2016-05-09T04:37:54Z","timestamp":1462768674000},"page":"445-450","source":"Crossref","is-referenced-by-count":10,"title":["Hepatitis B Surface Antigen S Gene is an Effective Carrier Molecule for Developing Gn<scp>RH DNA<\/scp> Immunocastration Vaccine in Mice"],"prefix":"10.1111","volume":"51","author":[{"given":"YG","family":"Han","sequence":"first","affiliation":[{"name":"Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of the Ministry of Education College of Animal Science and Technology Huazhong Agricultural University Wuhan 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