{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T20:21:13Z","timestamp":1790886073140,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,5,20]],"date-time":"2016-05-20T00:00:00Z","timestamp":1463702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NF\u00dc (National Development Agency)","award":["12-1-2012-0075"],"award-info":[{"award-number":["12-1-2012-0075"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Objectives were to determine associations between percentage pregnancy loss (<jats:styled-content style=\"fixed-case\">PPL<\/jats:styled-content>) in dairy cattle and: (i) pregnancy diagnosis by ultrasonography; (ii) pregnancy diagnosis by serum pregnancy\u2010specific protein B (<jats:styled-content style=\"fixed-case\">PSPB<\/jats:styled-content>) concentrations, with or without serum progesterone concentrations; and (iii) production and environmental factors. This study included 149\u00a0822 pregnancy diagnoses conducted over 13\u00a0years in Holstein\u2010Friesian cows in Hungarian dairy herds. The following were determined: <jats:styled-content style=\"fixed-case\">PPL<\/jats:styled-content> in cows diagnosed pregnant by transrectal ultrasonography 29\u201342\u00a0days after artificial insemination (<jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>; n\u00a0=\u00a011\u00a0457); <jats:styled-content style=\"fixed-case\">PPL<\/jats:styled-content> in cows diagnosed pregnant by serum <jats:styled-content style=\"fixed-case\">PSPB<\/jats:styled-content> 29\u201335\u00a0days after <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content> (n\u00a0=\u00a0138\u00a0365); and <jats:styled-content style=\"fixed-case\">PPL<\/jats:styled-content> and its association with serum progesterone concentrations, <jats:styled-content style=\"fixed-case\">PSPB<\/jats:styled-content> and production\/environmental variables. The definition of <jats:styled-content style=\"fixed-case\">PPL<\/jats:styled-content> was percentage of cows initially diagnosed pregnant based on ultrasonography or <jats:styled-content style=\"fixed-case\">PSPB<\/jats:styled-content>, but not pregnant when examined by transrectal palpation 60 \u201370\u00a0days after <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>. The <jats:styled-content style=\"fixed-case\">PPL<\/jats:styled-content> was lower (p\u00a0&lt;\u00a00.001) in cows following ultrasonographic vs <jats:styled-content style=\"fixed-case\">PSPB<\/jats:styled-content> diagnosis of pregnancy at 29\u201335\u00a0days (8.1 vs 19.3%, respectively), but was higher in cows following ultrasonographic pregnancy diagnosis on 29\u201335 vs 36\u201342\u00a0days (8.1 vs 7.1%, respectively, P\u00a0&lt;\u00a00.05). Furthermore, 72.9% of pregnancies with ultrasound\u2010detected morphological abnormalities resulted in pregnancy loss. As a subset of <jats:styled-content style=\"fixed-case\">PSPB<\/jats:styled-content> data, a fully quantitative <jats:styled-content style=\"fixed-case\">PSPB<\/jats:styled-content> assay was used for 20\u00a0430 samples; <jats:styled-content style=\"fixed-case\">PPL<\/jats:styled-content> in cows with a high <jats:styled-content style=\"fixed-case\">PSPB<\/jats:styled-content> concentration (&gt;1.1\u00a0ng\/ml) was lowest (15.0%), whereas cows with low concentrations of both <jats:styled-content style=\"fixed-case\">PSPB<\/jats:styled-content> and progesterone (0.6\u20131.1 and &lt;2\u00a0ng\/ml, respectively) had the highest <jats:styled-content style=\"fixed-case\">PPL<\/jats:styled-content> (76.3%; p\u00a0&lt;\u00a00.0001). Furthermore, <jats:styled-content style=\"fixed-case\">PPL<\/jats:styled-content> was higher in cows with advanced parity and with high milk production, when ambient temperatures were high, although body condition score (<jats:styled-content style=\"fixed-case\">BCS<\/jats:styled-content>) had no effect on <jats:styled-content style=\"fixed-case\">PPL<\/jats:styled-content>. Finally, there were no significant associations between serum <jats:styled-content style=\"fixed-case\">PSPB<\/jats:styled-content> and environmental temperatures or number of post\u2010partum uterine treatments.<\/jats:p>","DOI":"10.1111\/rda.12703","type":"journal-article","created":{"date-parts":[[2016,5,21]],"date-time":"2016-05-21T21:46:28Z","timestamp":1463867188000},"page":"467-473","source":"Crossref","is-referenced-by-count":45,"title":["Pregnancy Loss in Dairy Cattle: Relationship of Ultrasound, Blood Pregnancy\u2010Specific Protein B, Progesterone and Production Variables"],"prefix":"10.1111","volume":"51","author":[{"given":"G","family":"G\u00e1bor","sequence":"first","affiliation":[{"name":"National Agricultural Research and Innovation Centre Research Institute for Animal Breeding, Nutrition and Meat Science Herceghalom Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JP","family":"Kastelic","sequence":"additional","affiliation":[{"name":"Department of Production Animal Health Faculty of Veterinary Medicine University of Calgary Calgary, Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z","family":"Abonyi\u2010T\u00f3th","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Science Szent Istvan University G\u00f6d\u00f6ll\u0151 Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"G\u00e1bor","sequence":"additional","affiliation":[{"name":"Androvet Kft. Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Endr\u0151di","sequence":"additional","affiliation":[{"name":"Dennis G\u00e1bor Applied University Budapest Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"OG","family":"Balogh","sequence":"additional","affiliation":[{"name":"National Agricultural Research and Innovation Centre Research Institute for Animal Breeding, Nutrition and Meat Science Herceghalom Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(06)72581-4"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2478\/v10181-012-0112-5"},{"issue":"1","key":"e_1_2_7_4_1","first-page":"26","article-title":"Comparison of BioPRYN and a new pregnancy\u2010specific protein B (PSPB) enzyme\u2010linked immunosorbent assay (ELISA) for determination of early pregnancy status in dairy cattle","volume":"94","author":"Branen JR","year":"2011","journal-title":"J Dairy Sci"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod26.5.925"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(86)90030-8"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2006.08.008"},{"key":"e_1_2_7_8_1","first-page":"21","article-title":"Embryo mortality in cattle \u2013 prospects for improvement","volume":"21","author":"Diskin MG","year":"2013","journal-title":"Cattle Pract"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(99)00088-3"},{"key":"e_1_2_7_10_1","first-page":"459","article-title":"Ways of decrease the period between calvings in dairy cows. 1. 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