{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:21:36Z","timestamp":1758126096067},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,6,20]],"date-time":"2016-06-20T00:00:00Z","timestamp":1466380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004071","name":"Khon Kaen University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004071","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> fragmentation of frozen\u2010thawed feline epididymal sperm from corpus and cauda regions was evaluated by three different techniques. The <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> fragmentation index (<jats:styled-content style=\"fixed-case\">DFI<\/jats:styled-content>) was compared between techniques: the sperm chromatin structural assay (<jats:styled-content style=\"fixed-case\">SCSA<\/jats:styled-content><jats:sup>\u00ae<\/jats:sup>), acridine orange staining techniques (<jats:styled-content style=\"fixed-case\">AOT<\/jats:styled-content>) and the sperm chromatin dispersion (<jats:styled-content style=\"fixed-case\">SCD<\/jats:styled-content>). There were significant differences in <jats:styled-content style=\"fixed-case\">DFI<\/jats:styled-content> among the techniques (p\u00a0&lt;\u00a00.05) with no correlations. Only <jats:styled-content style=\"fixed-case\">DFI<\/jats:styled-content> values obtained from <jats:styled-content style=\"fixed-case\">SCD<\/jats:styled-content> revealed a significantly higher <jats:styled-content style=\"fixed-case\">DFI<\/jats:styled-content> in corpus compared with cauda spermatozoa (p\u00a0&lt;\u00a00.05). The discrepancy between techniques might be due to the sensitivity of each technique, differences in severity of <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damaged that can be detected. The difference in <jats:styled-content style=\"fixed-case\">DFI<\/jats:styled-content> between epididymal regions from <jats:styled-content style=\"fixed-case\">SCD<\/jats:styled-content> technique might indicate different maturational stages of spermatozoa, with less chromatin condensation of spermatozoa in corpus compared with cauda epididymis.<\/jats:p>","DOI":"10.1111\/rda.12710","type":"journal-article","created":{"date-parts":[[2016,6,20]],"date-time":"2016-06-20T10:54:10Z","timestamp":1466420050000},"page":"618-622","source":"Crossref","is-referenced-by-count":7,"title":["Comparison of <scp>DNA<\/scp> Fragmentation Assay in Frozen\u2010Thawed Cat Epididymal Sperm"],"prefix":"10.1111","volume":"51","author":[{"given":"P","family":"Kunkitti","sequence":"first","affiliation":[{"name":"Division of Reproduction Department of Clinical Sciences Faculty of Veterinary Medicine and Animal Science Swedish University of Agricultural Sciences Uppsala Sweden"},{"name":"Department of Surgery 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