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Furthermore, the research was performed also on the epididymal <jats:italic>cauda<\/jats:italic> of a senile buffalo bull with inactive testis. Aquaporins 1 and 9 were immunolocalized at distinct levels. In the efferent ducts, AQP1 immunoreactivity was strongly evidenced at the apical surface of the non\u2010ciliated cells and weakly along the basal membrane of the epithelial cells. The latter reactivity disappeared during the non\u2010mating season. No AQP1 immunoreactivity was detected in the epithelium of epididymis and <jats:italic>vas deferens<\/jats:italic>, whereas AQP1 was expressed in the smooth muscle layer of the <jats:italic>vas deferens<\/jats:italic>. Aquaporin 1 was present in the blood vessels and in small nerve bundles all along the genital tract. The supranuclear zone of the epididymal principal cells was AQP9 immunoreactive, limited to the <jats:italic>corpus<\/jats:italic> and <jats:italic>cauda<\/jats:italic> regions, and <jats:italic>vas deferens<\/jats:italic>. The samples collected in the two reproductive seasons showed a weaker AQP9 immunoreactivity during the non\u2010mating season. A typical AQP9 immunoreactivity was noticed in the old buffalo examined. The tested AQP molecules showed a different expression pattern in comparison with laboratory mammals, primates, equine, dog and cat. In addition, seasonal differences were noticed which are possibly useful in regard to the comprehension of the morphophysiology of reproduction in the bubaline species, which are still a matter of debate.<\/jats:p>","DOI":"10.1111\/rda.12713","type":"journal-article","created":{"date-parts":[[2016,6,4]],"date-time":"2016-06-04T04:26:47Z","timestamp":1465014407000},"page":"515-523","source":"Crossref","is-referenced-by-count":18,"title":["Seasonal and Ageing\u2010Depending Changes of Aquaporins 1 and 9 Expression in the Genital Tract of Buffalo Bulls <i>(Bubalus bubalis)<\/i>"],"prefix":"10.1111","volume":"51","author":[{"given":"S.","family":"Arrighi","sequence":"first","affiliation":[{"name":"Department of Health, Animal Science and Food Safety (VESPA) Laboratory of Anatomy Universit\u00e0 degli Studi di Milano Milano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Bosi","sequence":"additional","affiliation":[{"name":"Department of Health, Animal Science and Food Safety (VESPA) Laboratory of Anatomy Universit\u00e0 degli Studi di Milano Milano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Accogli","sequence":"additional","affiliation":[{"name":"Department of Emergency and Organ Transplantation (DETO) Veterinary Clinics and Animal Productions Section University of Bari \u201cAldo Moro\u201d Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Desantis","sequence":"additional","affiliation":[{"name":"Department of Emergency and Organ Transplantation (DETO) Veterinary Clinics and Animal Productions Section University of Bari \u201cAldo Moro\u201d Bari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0264.1985.tb00280.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.020818"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12213"},{"key":"e_1_2_8_5_1","first-page":"7","article-title":"Ultrastructural study on the epithelium lining ductus epididymis in adult cats (Felis catus)","volume":"97","author":"Arrighi S","year":"1986","journal-title":"Arch Biol"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0940-9602(11)80229-3"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.06.039"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/1877609401002010029"},{"key":"e_1_2_8_9_1","first-page":"433","article-title":"Absorptive activities of the efferent ducts evaluated by the immunolocalization of aquaporin water channels and lectin histochemistry in adult cats","volume":"25","author":"Arrighi S","year":"2010","journal-title":"Histol Histopathol"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2012.12.001"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12687"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2002.tb02243.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(19991201)256:4<335::AID-AR1>3.0.CO;2-I"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.1994.sp003817"},{"key":"e_1_2_8_15_1","first-page":"1","article-title":"Fluid and electrolyte reabsorption in the ductuli efferentes testis","volume":"53","author":"Clulow J","year":"1998","journal-title":"J Reprod Ferti. 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