{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T17:18:55Z","timestamp":1758820735280},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,6,23]],"date-time":"2016-06-23T00:00:00Z","timestamp":1466640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31402077"],"award-info":[{"award-number":["31402077"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007128","name":"Natural Science Foundation of Shaanxi Province","doi-asserted-by":"publisher","award":["2014JQ3096","2015JQ3064"],"award-info":[{"award-number":["2014JQ3096","2015JQ3064"]}],"id":[{"id":"10.13039\/501100007128","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CCAAT<\/jats:styled-content>\/enhancer\u2010binding protein beta (C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content><jats:italic>\u03b2<\/jats:italic>), an important transcriptional factor, plays a pivotal role in the regulation of female germ cell development. However, the role of C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content><jats:italic>\u03b2<\/jats:italic> on the development of male germ cells has not been reported. In this study, we constructed the recombinant adenovirus plasmids of bovine C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content><jats:italic>\u03b2<\/jats:italic> gene and harvested the subsequent adenoviruses, and then assessed the <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels of spermatogenesis\u2010related genes (real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) and secretion of inhibin B after 48\u00a0h of Ad\u2010C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content><jats:italic>\u03b2<\/jats:italic> recombinant adenovirus infection in bovine sertoli cells (<jats:styled-content style=\"fixed-case\">SC<\/jats:styled-content>s). We found that overexpression of exogenous <jats:italic>C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content>\u03b2<\/jats:italic> gene upregulated the <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expressions of spermatogenesis\u2010related genes, including <jats:italic>Pdgfa<\/jats:italic>,<jats:italic> Claudin<\/jats:italic>,<jats:italic> Caspase\u20103<\/jats:italic>,<jats:italic> Occludin<\/jats:italic>,<jats:italic> kit1<\/jats:italic> and <jats:italic>Cyclin E,<\/jats:italic> and decreased the <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels of <jats:italic>Cyclin D1<\/jats:italic>. Meanwhile, overexpression of exogenous <jats:italic>C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content>\u03b2<\/jats:italic> gene significantly increases the amounts of secreted inhibin B. In conclusion, the results indicate that the C\/<jats:styled-content style=\"fixed-case\">EBP<\/jats:styled-content><jats:italic>\u03b2<\/jats:italic> gene plays an important regulatory role in regulation of the cell cycle regulators and spermatogenesis\u2010related genes expression and function of bovine SCs.<\/jats:p>","DOI":"10.1111\/rda.12724","type":"journal-article","created":{"date-parts":[[2016,6,24]],"date-time":"2016-06-24T09:05:40Z","timestamp":1466759140000},"page":"591-596","source":"Crossref","is-referenced-by-count":4,"title":["Overexpression of C\/<scp>EBP<\/scp><i>\u03b2<\/i> Affects The Cell Cycle Regulators and Spermatogenesis Related Genes Expression And Function of Bovine Sertoli Cells"],"prefix":"10.1111","volume":"51","author":[{"given":"K","family":"Tang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Northwest A&amp;F University Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northwest A&amp;F University Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northwest A&amp;F University Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Northwest A&amp;F University Shaanxi 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