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The aim of this study was to identify oestrous\u2010specific bovine cervical mucus proteins that could be used to determine the optimal time for artificial insemination. Non\u2010oestrous and controlled internal drug release (<jats:styled-content style=\"fixed-case\">CIDR<\/jats:styled-content>)\u2010induced oestrous\u2010stage mucus proteins were purified and subjected to surface\u2010enhanced laser desorption\/ionization time\u2010of\u2010flight mass spectrometry, sodium dodecyl sulphate polyacrylamide gel electrophoresis and <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content>. Among differentially expressed proteins, lactoferrin (<jats:styled-content style=\"fixed-case\">LF<\/jats:styled-content>) and glutamate receptor\u2010interacting protein 1 (<jats:styled-content style=\"fixed-case\">GRIP<\/jats:styled-content>1) showed a twofold increase during the <jats:styled-content style=\"fixed-case\">CIDR<\/jats:styled-content>\u2010induced oestrous stage compared to the levels in non\u2010oestrous stage in bovine cervical mucus. The <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, Western blotting and immunohistochemistry results showed that <jats:styled-content style=\"fixed-case\">LF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GRIP<\/jats:styled-content>1 expression was significantly increased during the oestrous stage in the uterus. This study demonstrated that bovine <jats:styled-content style=\"fixed-case\">LF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GRIP<\/jats:styled-content>1 exist during the oestrous stage, but not during the non\u2010oestrous stage, suggesting that cervical mucus <jats:styled-content style=\"fixed-case\">LF<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GRIP<\/jats:styled-content>1 are useful oestrous detection markers in cattle.<\/jats:p>","DOI":"10.1111\/rda.12744","type":"journal-article","created":{"date-parts":[[2016,11,25]],"date-time":"2016-11-25T06:12:49Z","timestamp":1480054369000},"page":"16-23","source":"Crossref","is-referenced-by-count":6,"title":["Identification of lactoferrin and glutamate receptor\u2010interacting protein 1 in bovine cervical mucus: A putative marker for oestrous detection"],"prefix":"10.1111","volume":"52","author":[{"given":"WY","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Food Bioscience College of Biomedical &amp; Health Science Konkuk University  Chung\u2010ju Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MH","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Biomedical Chemistry College of Biomedical &amp; Health Science Konkuk University  Chung\u2010ju Korea"},{"name":"Doosan Venture Digm 924  Dongan\u2010gu Anyang Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KW","family":"Kim","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Division National Institute of Animal Science  Wanju\u2010gun Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology Konkuk University  Gwangjin\u2010gu Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KB","family":"Kim","sequence":"additional","affiliation":[{"name":"Korea Institute for Animal Products Quality Evaluation  Gunpo Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CS","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biomedical Chemistry College of Biomedical &amp; Health Science Konkuk University  Chung\u2010ju Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"NK","family":"Kim","sequence":"additional","affiliation":[{"name":"Experiment Research Institute National Agricultural Products Quality Management Service (NAQS)  Gimcheon Gyeongbuk Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JK","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Swine &amp; Poultry Science Korea National College of Agriculture and Fisheries  Jeonju Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BC","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Hanwoo National institute of animal Science  Pyeongchang Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KB","family":"Oh","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Division National Institute of Animal Science  Wanju\u2010gun Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GS","family":"Im","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Division National Institute of Animal Science  Wanju\u2010gun Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HJ","family":"Chung","sequence":"additional","affiliation":[{"name":"Animal Biotechnology Division National Institute of Animal Science  Wanju\u2010gun Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,11,25]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"619","article-title":"Proliferative changes in the genital tissue of female mice during the oestrous cycle","volume":"23","author":"Evans G. 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