{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:48:27Z","timestamp":1790671707944,"version":"4.1.0"},"reference-count":9,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,9,21]],"date-time":"2016-09-21T00:00:00Z","timestamp":1474416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001342","name":"Department of Science and Technology, Republic of South Africa","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001342","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>During the last decades, physiological effects of oestrogens have been increasingly explored by scientists and biotechnologists. Estrogens exert a wide range of effects on a large variety of cell types. Oestrogen and its receptors are essential for sexual development and reproduction. Estrogen receptor alpha is a nuclear receptor activated by the hormone oestrogen. In male,<jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content>\u03b1 is encoded by the gene estrogen receptor gene 1 (<jats:styled-content style=\"fixed-case\">ESR<\/jats:styled-content>1), responsible for better fertility. The<jats:styled-content style=\"fixed-case\">ESR<\/jats:styled-content>1 is involved in the reabsorption of luminal fluid during the transit of spermatozoa from the testis to the head of the epididymis which is important for their survival and maturation during epididymal storage. The absence of<jats:styled-content style=\"fixed-case\">ESR<\/jats:styled-content>1 leads to reduced epididymal sperm content, reduced sperm motility and fertilizing ability. Therefore, this is a good startby to study the expression pattern of estrogen receptor 1 gene in high\u2010fertile (G1) and low\u2010fertile (G2) bucks of Jamunapari and Barbari breeds identified on the basis of seminal quality traits and fertility trials.<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>was extracted from the tissues by<jats:styled-content style=\"fixed-case\">TRI<\/jats:styled-content>zol method. The identification and expression pattern of caprine<jats:styled-content style=\"fixed-case\">ESR<\/jats:styled-content>1 gene was analysed by real\u2010time<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>(Roche<jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010480). Our work shows that the relative quantification by<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>indicates more fold in head of epididymis as compared to spleen of caprine<jats:styled-content style=\"fixed-case\">ESR<\/jats:styled-content>1 gene. Furthermore, the<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>indicated that fertile bucks of Jamunapari breed have more fold value as compared to Barbari breed in respect of reproductive organ.<\/jats:p>","DOI":"10.1111\/rda.12774","type":"journal-article","created":{"date-parts":[[2016,9,21]],"date-time":"2016-09-21T09:09:12Z","timestamp":1474448952000},"page":"1049-1054","source":"Crossref","is-referenced-by-count":5,"title":["Molecular expression of caprine estrogen receptor gene 1 in reproductive and non\u2010reproductive tissues"],"prefix":"10.1111","volume":"51","author":[{"given":"S","family":"Saraswat","sequence":"first","affiliation":[{"name":"ICAR\u2010Central Institute for Research on goats Mathura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PK","family":"Rout","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute for Research on goats Mathura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SD","family":"Kharche","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute for Research on goats Mathura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SK","family":"Jindal","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute for Research on goats Mathura India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AK","family":"Goel","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute for Research on goats Mathura India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,9,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.17.4688"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acthis.2004.04.002"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.08.008"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1002\/j.1939-4640.1997.tb02437.x","article-title":"Estrogen receptor (alpha and beta) expression in the excurrent ducts of the adult male rat reproductive tract","volume":"18","author":"Hess R.","year":"1997","journal-title":"Journal of Andrology"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0070926"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2010.01.002"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/boc.201100109"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.2014.14380"},{"key":"e_1_2_10_10_1","first-page":"47","article-title":"Standardization of antioxidants fortification in frozen buck semen","volume":"18","author":"Saraswat S.","year":"2012","journal-title":"Indian Journal of Small Ruminants"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12774","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12774","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T13:50:52Z","timestamp":1718805052000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12774"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,21]]},"references-count":9,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1111\/rda.12774"],"URL":"https:\/\/doi.org\/10.1111\/rda.12774","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,21]]}}}