{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T15:28:45Z","timestamp":1759937325230},"reference-count":60,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,9,20]],"date-time":"2016-09-20T00:00:00Z","timestamp":1474329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["1130888","3150320"],"award-info":[{"award-number":["1130888","3150320"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Short\u2010term exposure of gametes to different types of stress might induce stress tolerance in mammalian embryos. The aim of this study was to evaluate the effect of short\u2010term exposure of bovine mature cumulus\u2013oocyte complex (<jats:styled-content style=\"fixed-case\">COC<\/jats:styled-content>) to 3\u2010morpholinosydnonimine (<jats:styled-content style=\"fixed-case\">SIN<\/jats:styled-content>\u20101) on subsequent in vitro embryo development, embryo quality and relative gene expression. Matured <jats:styled-content style=\"fixed-case\">COC<\/jats:styled-content>s were incubated with <jats:styled-content style=\"fixed-case\">SIN<\/jats:styled-content>\u20101 (0, 0.1, 1, 10 and 100\u00a0\u03bcM <jats:styled-content style=\"fixed-case\">SIN<\/jats:styled-content>\u20101) for 1\u00a0hr before in vitro fertilization and zygotes were cultured until Day 7. The cleavage rate at 72\u00a0hr did not show any differences among groups. However, the blastocyst rate on Day 7 decreased with all treatments evaluated, with the embryos generated with 10\u00a0\u03bcM <jats:styled-content style=\"fixed-case\">SIN<\/jats:styled-content>\u20101 showing the lowest embryo production rate. Embryo quality analysis did not show any differences in total cell number (<jats:styled-content style=\"fixed-case\">TCN<\/jats:styled-content>) or inner cell mass (<jats:styled-content style=\"fixed-case\">ICM<\/jats:styled-content>) among groups. Relative gene expression analysis showed a downregulation of <jats:italic><jats:styled-content style=\"fixed-case\">eNOS<\/jats:styled-content><\/jats:italic> expression and an upregulation of <jats:italic><jats:styled-content style=\"fixed-case\">nNOS<\/jats:styled-content><\/jats:italic> expression in all treatments evaluated compared to the control group. Also, a downregulation was observed in some treatments: <jats:italic><jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content>2<\/jats:italic> at 0.1\u00a0\u03bcM; <jats:italic><jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content>1<\/jats:italic> at 0.1 and 100\u00a0\u03bcM; <jats:italic><jats:styled-content style=\"fixed-case\">PRDX<\/jats:styled-content>5<\/jats:italic> at 0.1, 10 and 100\u00a0\u03bcM; and <jats:italic><jats:styled-content style=\"fixed-case\">NANOG<\/jats:styled-content><\/jats:italic> at 10 and 100\u00a0\u03bcM; and an upregulation of <jats:italic><jats:styled-content style=\"fixed-case\">CDX<\/jats:styled-content>2<\/jats:italic> expression was observed at 100\u00a0\u03bcM. The other genes (<jats:italic><jats:styled-content style=\"fixed-case\">OCT<\/jats:styled-content>4<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">HIF<\/jats:styled-content>1A<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">HSPA<\/jats:styled-content>1A<\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">BCL<\/jats:styled-content>2A<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">iNOS<\/jats:styled-content><\/jats:italic>) did not show any differences in the relative gene expression. These results suggest that the short\u2010term exposure of mature bovine <jats:styled-content style=\"fixed-case\">COC<\/jats:styled-content>s to <jats:styled-content style=\"fixed-case\">SIN<\/jats:styled-content>\u20101 does not induce stress tolerance and has no beneficial effect on bovine in vitro embryo production.<\/jats:p>","DOI":"10.1111\/rda.12788","type":"journal-article","created":{"date-parts":[[2016,9,20]],"date-time":"2016-09-20T05:27:19Z","timestamp":1474349239000},"page":"1010-1019","source":"Crossref","is-referenced-by-count":3,"title":["Effect of short\u2010term exposure of cumulus\u2013oocyte complex to 3\u2010morpholinosydnonimine on in vitro embryo development and gene expression in cattle"],"prefix":"10.1111","volume":"51","author":[{"given":"P","family":"Loren","sequence":"first","affiliation":[{"name":"Student of Doctoral Program in Sciences major in Applied Cellular and Molecular Biology Universidad de la Frontera  Temuco Chile"},{"name":"Centre of Biotechnology on Reproduction (CEBIOR\u2010BIOREN) Faculty of Medicine Universidad de La Frontera  Temuco Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Cheuquem\u00e1n","sequence":"additional","affiliation":[{"name":"Centre of Biotechnology on Reproduction (CEBIOR\u2010BIOREN) Faculty of Medicine Universidad de La Frontera  Temuco Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Student of Doctoral Program in Sciences major in Applied Cellular and Molecular Biology Universidad de la Frontera  Temuco Chile"},{"name":"Centre of Biotechnology on Reproduction (CEBIOR\u2010BIOREN) Faculty of Medicine Universidad de La Frontera  Temuco Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Risopatr\u00f3n","sequence":"additional","affiliation":[{"name":"Centre of Biotechnology on Reproduction (CEBIOR\u2010BIOREN) Faculty of Medicine Universidad de La Frontera  Temuco Chile"},{"name":"Department of Basic Science Faculty of Medicine Universidad de La Frontera  Temuco Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ME","family":"Arias","sequence":"additional","affiliation":[{"name":"Centre of Biotechnology on Reproduction (CEBIOR\u2010BIOREN) Faculty of Medicine Universidad de La Frontera  Temuco Chile"},{"name":"Department of Agricultural and Livestock Production Faculty of Farming Livestock and Forestry Sciences Universidad de La Frontera  Temuco Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Felmer","sequence":"additional","affiliation":[{"name":"Centre of Biotechnology on Reproduction (CEBIOR\u2010BIOREN) Faculty of Medicine Universidad de La Frontera  Temuco Chile"},{"name":"Department of Agricultural and Livestock Production Faculty of Farming Livestock and Forestry Sciences Universidad de La Frontera  Temuco Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Centre of Biotechnology on Reproduction (CEBIOR\u2010BIOREN) Faculty of Medicine Universidad de La Frontera  Temuco Chile"},{"name":"Department of Preclinical Sciences Faculty of Medicine Universidad de La Frontera  Temuco 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