{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:18:27Z","timestamp":1790968707788,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,9,20]],"date-time":"2016-09-20T00:00:00Z","timestamp":1474329600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2012\/05\/E\/NZ9\/03480"],"award-info":[{"award-number":["2012\/05\/E\/NZ9\/03480"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004895","name":"European Social Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004895","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Lysophosphatidic acid (<jats:styled-content style=\"fixed-case\">LPA<\/jats:styled-content>) exerts various actions on the mammalian reproductive system. In cows, <jats:styled-content style=\"fixed-case\">LPA<\/jats:styled-content> stimulates the synthesis and secretion of luteotropic factors in the ovary, which affects the growth and development of ovarian follicles. The role of <jats:styled-content style=\"fixed-case\">LPA<\/jats:styled-content> in granulosa cells, oocyte and oocyte\u2010cumulus complex (<jats:styled-content style=\"fixed-case\">COC<\/jats:styled-content>) has previously been investigated; but its role in the theca layer, which is an important structural and functional component of the ovarian follicle, is still unclear. The goal of this study was to investigate the expression of <jats:styled-content style=\"fixed-case\">LPA<\/jats:styled-content> in theca cells originating from different bovine ovarian follicle types. Theca cells were separated from healthy, transitional and atretic ovarian follicles, based on intrafollicular estradiol: progesterone ratios. <jats:styled-content style=\"fixed-case\">LPA<\/jats:styled-content> concentration in the follicular fluid (<jats:styled-content style=\"fixed-case\">FF<\/jats:styled-content>) in different follicle types was measured, and expression of the enzymes responsible for <jats:styled-content style=\"fixed-case\">LPA<\/jats:styled-content> synthesis (autotaxin [<jats:styled-content style=\"fixed-case\">AX<\/jats:styled-content>], phospholipase A2 [<jats:styled-content style=\"fixed-case\">PLA<\/jats:styled-content>2]) and receptors for <jats:styled-content style=\"fixed-case\">LPA<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">LPAR<\/jats:styled-content>1\u20104) were determined. The obtained results confirmed the follicle\u2010type dependent presence of <jats:styled-content style=\"fixed-case\">LPA<\/jats:styled-content> in the <jats:styled-content style=\"fixed-case\">FF<\/jats:styled-content> of the bovine ovarian follicles. The highest concentration of <jats:styled-content style=\"fixed-case\">LPA<\/jats:styled-content> was detected in follicles classified as healthy and dominant. <jats:styled-content style=\"fixed-case\">LPAR<\/jats:styled-content>1\u20104, <jats:styled-content style=\"fixed-case\">PLA<\/jats:styled-content>2 and <jats:styled-content style=\"fixed-case\">AX<\/jats:styled-content> expression in theca cells in all of the types of follicles examined were detected at <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and protein level. These results suggest that theca cells can be a source of <jats:styled-content style=\"fixed-case\">LPA<\/jats:styled-content> synthesis other than granulosa cells and <jats:styled-content style=\"fixed-case\">COC<\/jats:styled-content>s, as well as the target for its action in the bovine ovarian follicle, with <jats:styled-content style=\"fixed-case\">PLA<\/jats:styled-content>2 and <jats:styled-content style=\"fixed-case\">LPAR<\/jats:styled-content>4 playing major roles in <jats:styled-content style=\"fixed-case\">LPA<\/jats:styled-content> synthesis and action.<\/jats:p>","DOI":"10.1111\/rda.12790","type":"journal-article","created":{"date-parts":[[2016,9,20]],"date-time":"2016-09-20T05:52:36Z","timestamp":1474350756000},"page":"28-34","source":"Crossref","is-referenced-by-count":11,"title":["Lysophosphatidic acid expression in theca cells depends on the type of bovine ovarian 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