{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T04:11:51Z","timestamp":1767845511780,"version":"3.49.0"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,11,11]],"date-time":"2016-11-11T00:00:00Z","timestamp":1478822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["BID\u2010PICT\u20102008\u20102210"],"award-info":[{"award-number":["BID\u2010PICT\u20102008\u20102210"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003947","name":"Universidad Nacional de La Plata","doi-asserted-by":"publisher","award":["V\/229"],"award-info":[{"award-number":["V\/229"]}],"id":[{"id":"10.13039\/501100003947","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The zonary and endotheliochorial dog placenta is the most invasive placenta of carnivores. The importance of matrix metalloproteinases (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>) in placenta invasiveness has been determined in several mammals including species with haemochorial, epitheliochorial and endotheliochorial placentation. Regarding the latter, the expression of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content> enzymes has been studied in the cat and the mature canine placenta. The aim of this study was to analyse the expression and activity of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20102 and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 in the early dog placenta. Placentae from 18 to 30\u00a0days of pregnancy were collected from four bitches. Two placentae from each bitch were analysed. Placental tissue from one uterine horn was fixed in formaldehyde for immunohistochemistry, while marginal haematoma, labyrinth, non\u2010implantative and implantative endometrium from the contralateral horn were immediately frozen in dry ice for the analysis of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content> expression (Western blot [<jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content>]) and activity (zymography). <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20102 and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 were evidenced in the labyrinth, maternal glands and marginal haematoma; this finding was directly correlated with levels of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content> expression by <jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content>, and with the activity of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20102, mainly in the haematoma (the area of major remodelling of tissues). Thus, although <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 is well expressed in the early canine placenta, it is not active. Given the important role of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>s for invasiveness, maternal\u2013foetal angiogenesis and the establishment of a correct foetal nutrition, the results are consistent with the findings in other species in which the <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20102 activation precedes the <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20109 one in early placentation.<\/jats:p>","DOI":"10.1111\/rda.12791","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T15:56:38Z","timestamp":1479311798000},"page":"35-43","source":"Crossref","is-referenced-by-count":13,"title":["Differential expression and activity of matrix metalloproteinases 2 and 9 in canine early placenta"],"prefix":"10.1111","volume":"52","author":[{"given":"M","family":"Diessler","sequence":"first","affiliation":[{"name":"Laboratorio de Histolog\u00eda y Embriolog\u00eda Descriptiva, Experimental y Comparada (LHYEDEC) Facultad de Ciencias Veterinarias Universidad Nacional de La Plata (UNLP)  La Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Ventureira","sequence":"additional","affiliation":[{"name":"Laboratorio de Reproducci\u00f3n y Fisiopatolog\u00eda Materno\u2010Embrionaria Instituto de Fisiolog\u00eda, Biolog\u00eda Molecular y Neurociencias (IFIBYNE) Universidad de Buenos Aires (UBA)\u2010 Comisi\u00f3n Nacional de Investigaciones Cient\u00edficas (CONICET) Facultad de Ciencias Exactas y Naturales (FCEyN)\u2010UBA  Buenos Aires Argentina"},{"name":"Instituto de Investigaciones Biom\u00e9dicas (INBIOMED)\u2010UBA\/CONICET Facultad de Medicina UBA  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Hernandez","sequence":"additional","affiliation":[{"name":"Laboratorio de Histolog\u00eda y Embriolog\u00eda Descriptiva, Experimental y Comparada (LHYEDEC) Facultad de Ciencias Veterinarias Universidad Nacional de La Plata (UNLP)  La Plata Argentina"},{"name":"Instituto de Investigaciones Biom\u00e9dicas (INBIOMED)\u2010UBA\/CONICET Facultad de Medicina UBA  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Sobarzo","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Instituto de Investigaciones Biom\u00e9dicas (INBIOMED)\u2010UBA\/CONICET UBA  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Casas","sequence":"additional","affiliation":[{"name":"Small Animal Private Practice  La Plata Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Barbeito","sequence":"additional","affiliation":[{"name":"Laboratorio de Histolog\u00eda y Embriolog\u00eda Descriptiva, Experimental y Comparada (LHYEDEC) Facultad de Ciencias Veterinarias Universidad Nacional de La Plata (UNLP)  La Plata Argentina"},{"name":"Instituto de Investigaciones Biom\u00e9dicas (INBIOMED)\u2010UBA\/CONICET Facultad de Medicina UBA  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Cebral","sequence":"additional","affiliation":[{"name":"Laboratorio de Reproducci\u00f3n y Fisiopatolog\u00eda Materno\u2010Embrionaria Instituto de Fisiolog\u00eda, Biolog\u00eda Molecular y Neurociencias (IFIBYNE) Universidad de Buenos Aires (UBA)\u2010 Comisi\u00f3n Nacional de Investigaciones Cient\u00edficas (CONICET) Facultad de Ciencias Exactas y Naturales (FCEyN)\u2010UBA  Buenos Aires Argentina"},{"name":"Instituto de Investigaciones Biom\u00e9dicas (INBIOMED)\u2010UBA\/CONICET Facultad de Medicina UBA  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,11,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2013.02.003"},{"key":"e_1_2_9_3_1","first-page":"229","article-title":"Immunohistochemical and morphometric study of the decidual cells in the domestic cat placenta","volume":"28","author":"Barbeito C. G.","year":"2004","journal-title":"Biocell"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001430106"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00838.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12452"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7985020"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-006-7886-9"},{"key":"e_1_2_9_9_1","doi-asserted-by":"crossref","first-page":"S592","DOI":"10.1016\/j.placenta.2011.10.009","article-title":"Review: The evolving placenta: Different developmental paths to a hemochorial relationship","volume":"33","author":"Enders A. C.","year":"2012","journal-title":"Placenta"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2012.02.008"},{"issue":"1","key":"e_1_2_9_11_1","first-page":"11","article-title":"Prenatal development of domestic and laboratory mammals: Growth curves, external features and selected references","volume":"2","author":"Evans H. E.","year":"1973","journal-title":"Zentralblatt f\u00fcr Veterin\u00e4rmedizin"},{"key":"e_1_2_9_12_1","first-page":"1","article-title":"Intermediate filament proteins expression and sugar moieties of the normal canine placenta","volume":"15","author":"Fern\u00e1ndez P. E.","year":"2000","journal-title":"Histology and Histopathology"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12265"},{"key":"e_1_2_9_14_1","first-page":"995","article-title":"Characterization of cytotrophoblastic\u2010like cells present in subinvolutioned placental sites of the bitch","volume":"13","author":"Fern\u00e1ndez P. E.","year":"1998","journal-title":"Histology and Histopathology"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2015.09.020"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958\u2010015\u20100066\u20104"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12037"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719113519170"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2005.06.012"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsgi.2003.08.005"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.02.027"},{"key":"e_1_2_9_22_1","first-page":"87","article-title":"Ontogeny of the granulocyte\/macrophage progenitor cell (GM\u2010CFC) pools in the Beagle","volume":"80","author":"Nothdurft W.","year":"1984","journal-title":"Journal of Embryology and Experimental Morphology"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(02)04240-1"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004290050210"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12539"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/5.4.376"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1530\/reprod\/118.1.19"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-2-59"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.17.1.463"},{"key":"e_1_2_9_30_1","first-page":"291","article-title":"Scanning electron microscopy of the canine placenta","volume":"103","author":"Stoffel M. H.","year":"1998","journal-title":"Italian Journal of Anatomy and Embryology"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20637"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199903)214:3<248::AID-AJA8>3.0.CO;2-N"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2004.03.007"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod53.4.800"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4004(98)91037-2"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.04.038"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1053\/plac.2001.0633"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2005.02.014"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod65.1.31"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.3109\/09513590903184100"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-78797-6_7"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod62.4.988"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2005.05.014"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12791","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12791","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T16:53:25Z","timestamp":1695574405000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12791"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,11]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,2]]}},"alternative-id":["10.1111\/rda.12791"],"URL":"https:\/\/doi.org\/10.1111\/rda.12791","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,11]]}}}