{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T08:32:46Z","timestamp":1784190766138,"version":"3.55.0"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,11,13]],"date-time":"2016-11-13T00:00:00Z","timestamp":1478995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Warmia and Mazury University in Olsztyn","award":["11.610.003-300"],"award-info":[{"award-number":["11.610.003-300"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Heat shock proteins (<jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>s) act as molecular chaperones with important regulatory functions. <jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>s are considered to be essential factors in animal reproduction. In view of seasonal variations in the secretory activity of the reproductive tract of mature roe deer (<jats:italic>Capreolus capreolus<\/jats:italic>), the aims of this study were to identify <jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>s in the epididymides and compare the expression of the identified proteins in three periods of the reproductive season. Two\u2010dimensional polyacrylamide gel electrophoresis revealed the highest number of polypeptides in homogenates of epididymal tissues and in caput, corpus and cauda epididymal fluids throughout the reproductive season. Epididymal tissue homogenates and epididymal fluids were analysed by tandem mass spectrometry (<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>) to reveal 31 polypeptides with enzymatic activity, including polypeptides with antioxidant properties, structural and cell signalling functions. Moreover, among the identified polypeptides, five of them were similar to heat shock proteins: endoplasmin (Grp94); heat shock protein 90 <jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>a (<jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>90); 78\u2010<jats:styled-content style=\"fixed-case\">kD<\/jats:styled-content>a glucose\u2010regulated protein (Grp78); chain A, the crystal structure of the human <jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>70 <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>ase domain and heat shock protein beta\u20101 isoform X. The concentrations of the analysed polypeptides, expressed in optical density units (<jats:styled-content style=\"fixed-case\">ODU<\/jats:styled-content>), differed significantly (<jats:italic>p<\/jats:italic>\u00a0\u2264\u00a0.05) across the examined periods of the reproductive season. The highest <jats:styled-content style=\"fixed-case\">ODU<\/jats:styled-content> values for almost all analysed proteins were observed during the rutting period. The presence of <jats:styled-content style=\"fixed-case\">HSP<\/jats:styled-content>s in the epididymal tissues and fluids of roe deer in different periods of the reproductive season could indicate that those proteins play an important role in sperm maturation in the epididymis.<\/jats:p>","DOI":"10.1111\/rda.12816","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T14:34:57Z","timestamp":1479306897000},"page":"107-114","source":"Crossref","is-referenced-by-count":5,"title":["Identification and changes in the seasonal concentrations of heat shock proteins in roe deer (<i>Capreolus capreolus<\/i>) epididymides"],"prefix":"10.1111","volume":"52","author":[{"given":"AM","family":"Majewska","sequence":"first","affiliation":[{"name":"Department of Animal Biochemistry and Biotechnology University of Warmia and Mazury in Olsztyn  Olsztyn Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W","family":"Kordan","sequence":"additional","affiliation":[{"name":"Department of Animal Biochemistry and Biotechnology University of Warmia and Mazury in Olsztyn  Olsztyn Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Koziorowska\u2010Gilun","sequence":"additional","affiliation":[{"name":"Department of Animal Biochemistry and Biotechnology University of Warmia and Mazury in Olsztyn  Olsztyn Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P","family":"Wysocki","sequence":"additional","affiliation":[{"name":"Department of Animal Biochemistry and Biotechnology University of Warmia and Mazury in Olsztyn  Olsztyn 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