{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T02:21:26Z","timestamp":1776306086270,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"S2","license":[{"start":{"date-parts":[[2016,10,23]],"date-time":"2016-10-23T00:00:00Z","timestamp":1477180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Uterine tissue was collected from bitches after ovariohysterectomy at different times after ovulation. Samples were assigned to four groups: metestrous non\u2010pregnant, day 10\u201312, <jats:italic>n<\/jats:italic>\u00a0=\u00a04; pre\u2010implantation, day 10\u201312, <jats:italic>n<\/jats:italic>\u00a0=\u00a09; post\u2010implantation, day 18\u201325, <jats:italic>n<\/jats:italic>\u00a0=\u00a013; mid\u2010gestation, day 30\u201340, <jats:italic>n<\/jats:italic>\u00a0=\u00a07. <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> detection was performed for <jats:italic>kiss1<\/jats:italic> and the G protein\u2010coupled receptor 54 (<jats:italic><jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>54,<\/jats:italic> specific receptor for kisspeptin). In addition, immunohistochemistry was performed for detection of kisspeptin\u201010 (<jats:styled-content style=\"fixed-case\">KP<\/jats:styled-content>\u201010), <jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>54, as well as pan\u2010cytokeratin and vimentin. The latter two were included to differentiate the different placental cell types. The percentage of positive stained cells was evaluated, and an immunoreactivity score (<jats:styled-content style=\"fixed-case\">IRS<\/jats:styled-content>) was obtained by multiplying the labelling intensity score (0\u20133) with the percentage of immunolabelled cells (range: 0\u2013300). In non\u2010pregnant and pre\u2010implantation tissues, gene expression was highly variable for <jats:italic>kiss1<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>54<\/jats:italic>. Expression of <jats:italic><jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>54<\/jats:italic> was higher before embryo adhesion than during post\u2010implantation and mid\u2010gestation (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05), whereas there was no difference found between groups for <jats:italic>kiss1<\/jats:italic>. Except during the pre\u2010implantation period, <jats:styled-content style=\"fixed-case\">KP<\/jats:styled-content>\u201010 expression was higher in the non\u2010pregnant uterus compared to all gestational periods investigated, indicating a pregnancy\u2010related downregulation. In the pre\u2010implantation period, <jats:styled-content style=\"fixed-case\">KP<\/jats:styled-content>\u201010 was present in larger vessels only, whereas the presence of <jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>54 in vessels was found in all samples, with most labelling in the post\u2010implantation period. <jats:styled-content style=\"fixed-case\">KP<\/jats:styled-content>\u201010 was present in superficial uterine glands, <jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>54 in superficial and deep uterine glands of the post\u2010implantation uterus. In myocytes, the highest staining for <jats:styled-content style=\"fixed-case\">KP<\/jats:styled-content>\u201010 was seen in the non\u2010pregnant uterus, whereas the highest staining for <jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>54 was seen in post\u2010implantation and mid\u2010gestation. Syncytiotrophoblast cells stained for both <jats:styled-content style=\"fixed-case\">KP<\/jats:styled-content>\u201010 and <jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>54 in post\u2010implantation and mid\u2010gestation, with maximum intensity for <jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>54 in the latter. We conclude that <jats:styled-content style=\"fixed-case\">KP<\/jats:styled-content>\u201010 and <jats:styled-content style=\"fixed-case\">GPR<\/jats:styled-content>54 are expressed in the canine uterus and trophoblast cells. However, during pregnancy, expression of both proteins seems to be differentially regulated.<\/jats:p>","DOI":"10.1111\/rda.12818","type":"journal-article","created":{"date-parts":[[2016,10,24]],"date-time":"2016-10-24T04:52:44Z","timestamp":1477284764000},"page":"123-129","source":"Crossref","is-referenced-by-count":21,"title":["Kisspeptin\u201010 and the G protein\u2010coupled receptor 54 are differentially expressed in the canine pregnant uterus and trophoblast cells"],"prefix":"10.1111","volume":"52","author":[{"given":"S","family":"Sch\u00e4fer\u2010Somi","sequence":"first","affiliation":[{"name":"Platform for Artificial Insemination and Embryo Transfer Vetmeduni Vienna Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SS","family":"Ay","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology Faculty of Veterinary Medicine Ondokuz Mayis University Samsun 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