{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T05:35:41Z","timestamp":1783488941675,"version":"3.55.0"},"reference-count":29,"publisher":"Wiley","issue":"S2","license":[{"start":{"date-parts":[[2016,11,15]],"date-time":"2016-11-15T00:00:00Z","timestamp":1479168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["31003A_160251"],"award-info":[{"award-number":["31003A_160251"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The apparent lack of classical mechanisms for maternal recognition of pregnancy is one of the most intriguing features of canine reproduction. Consequently, similar levels of circulating luteal steroids are observed in pregnant and non\u2010pregnant dogs. However, the early pre\u2010implantation canine embryo locally modulates uterine responses to its presence, facilitating the successful onset of pregnancy. As a part of this interaction, the canine uterus undergoes a species\u2010specific decidualization. Maternal stroma\u2010derived decidual cells develop, the only cells of the canine placenta expressing progesterone receptor (<jats:styled-content style=\"fixed-case\">PGR<\/jats:styled-content>). There exists an acute need for an in vitro stable cell line model for canine decidualization. Therefore, herein our goal was to establish, immortalize and characterize such a cell line. We immortalized three monolayer dog uterine stromal (<jats:styled-content style=\"fixed-case\">DUS<\/jats:styled-content>) cell lines by stably transfecting them with<jats:styled-content style=\"fixed-case\">SV<\/jats:styled-content>40Tag oncogene. Cells retained their mesenchymal character for over 30 passages, as evidenced by<jats:styled-content style=\"fixed-case\">VIMENTIN<\/jats:styled-content>staining. Genomic incorporation of the<jats:styled-content style=\"fixed-case\">SV<\/jats:styled-content>40Tag protein was confirmed by immunofluorescence and Western blot analyses. Cells submitted to a classical in vitro decidualization protocol (N6,2\u2032\u2010O\u2010dibutyryladenosine\u20103\u2032,5\u2032\u2010cyclic monophosphate) revealed upregulated gene levels of selected major decidualization markers (<jats:italic>e.g<\/jats:italic>.<jats:italic>PRLR<\/jats:italic>,<jats:italic><jats:styled-content style=\"fixed-case\">PGR<\/jats:styled-content><\/jats:italic>,<jats:italic><jats:styled-content style=\"fixed-case\">IGF<\/jats:styled-content>1<\/jats:italic>,<jats:italic><jats:styled-content style=\"fixed-case\">PTGES<\/jats:styled-content><\/jats:italic>). Additionally, the basic decidualization capability of<jats:styled-content style=\"fixed-case\">PGE<\/jats:styled-content>2 was demonstrated, revealing increased levels of, for example,<jats:italic><jats:styled-content style=\"fixed-case\">PGR<\/jats:styled-content><\/jats:italic>and<jats:italic>PRLR<\/jats:italic>gene expression, thereby implying its involvement in the progesterone\u2010dependent decidualization in the canine uterus. In summary, our in vitro model with immortalized<jats:styled-content style=\"fixed-case\">DUS<\/jats:styled-content>cell line could serve as an ideal and unique model to study the underlying molecular and endocrine mechanisms of canine decidualization.<\/jats:p>","DOI":"10.1111\/rda.12849","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T18:08:14Z","timestamp":1479319694000},"page":"137-147","source":"Crossref","is-referenced-by-count":30,"title":["Decidualization of the canine uterus: From early until late gestational in vivo morphological observations<i>,<\/i>and functional characterization of immortalized canine uterine stromal cell lines"],"prefix":"10.1111","volume":"52","author":[{"given":"FR","family":"Graubner","sequence":"first","affiliation":[{"name":"Institute of Veterinary Anatomy Vetsuisse Faculty University of Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"IM","family":"Reichler","sequence":"additional","affiliation":[{"name":"Clinic of Reproductive Medicine Vetsuisse Faculty University of Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"NA","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Physiology Institute of Biomedicine University of Turku Turku Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5225-4510","authenticated-orcid":false,"given":"R","family":"Payan\u2010Carreira","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancia Animal e Veterin\u00e1ria (CECAV) Zootecnia Department University of Tr\u00e1s\u2010os\u2010Montes and Alto Douro Vila Real Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Boos","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Anatomy Vetsuisse Faculty University of Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MP","family":"Kowalewski","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Anatomy Vetsuisse Faculty University of Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,11,15]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"39","article-title":"Physiological mechanisms of pregnancy recognition in ruminants","volume":"43","author":"Bazer F. 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