{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T18:18:17Z","timestamp":1775240297214,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"S2","license":[{"start":{"date-parts":[[2016,11,22]],"date-time":"2016-11-22T00:00:00Z","timestamp":1479772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002873","name":"Chulalongkorn University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The Indochinese leopard (<jats:italic>Panthera pardus delacouri<\/jats:italic>) population, included in<jats:styled-content style=\"fixed-case\">CITES<\/jats:styled-content>Appendix I, has been declining for decades. Proper gamete preservation condition is critical for breeding programme management using artificial insemination or in vitro fertilization (<jats:styled-content style=\"fixed-case\">IVF<\/jats:styled-content>). The present study aimed at investigating the impact of post\u2010thawing treatment of leopard semen with extracellular adenosine 5\u2032\u2010triphosphate (<jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>e) on sperm quality (including morphological traits and ability to fertilize an oocyte). Semen from six adult male leopards was collected by electroejaculation (one ejaculation per cat). After the evaluation of the fresh sample quality, the semen was cryopreserved (10\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>cells per straw; two straws per cat). After thawing, the sperm sample from the first straw of each cat was divided into three aliquots: control (no<jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>e), supplemented with 1.0 or 2.5<jats:styled-content style=\"fixed-case\">mM ATP<\/jats:styled-content>e that were evaluated for sperm quality at 10, 30\u00a0min and 3\u00a0hr post\u2010thawing. The sperm sample from the second straw, supplemented with 0, 1.0 or 2.5<jats:styled-content style=\"fixed-case\">mM ATP<\/jats:styled-content>e for 30\u00a0min, was assessed for<jats:styled-content style=\"fixed-case\">IVF<\/jats:styled-content>with domestic cat oocytes. Sperm quality (all metrics) was negatively affected by the cryopreservation process (<jats:italic>p\u00a0<\/jats:italic>\u2264<jats:italic>\u00a0<\/jats:italic>.05). However, the percentage of sperm motility, level of progressive motility and percentage of plasma membrane integrity did not differ (<jats:italic>p\u00a0<\/jats:italic>&gt;<jats:italic>\u00a0<\/jats:italic>.05) among post\u2010thawing groups. The sperm mitochondrial membrane potential was enhanced (<jats:italic>p\u00a0<\/jats:italic>\u2264<jats:italic>\u00a0<\/jats:italic>.05) by<jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>e treatment (1.0 and 2.5<jats:styled-content style=\"fixed-case\">mM<\/jats:styled-content>; 10\u00a0min to 3\u00a0hr of incubation). Furthermore, incubation of<jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>e (1.0 and 2.5<jats:styled-content style=\"fixed-case\">mM<\/jats:styled-content>) for 30\u00a0min could promote sperm velocity patterns (curvilinear velocity;<jats:styled-content style=\"fixed-case\">VCL<\/jats:styled-content>and straight line velocity;<jats:styled-content style=\"fixed-case\">VSL<\/jats:styled-content>) (<jats:italic>p\u00a0<\/jats:italic>\u2264<jats:italic>\u00a0<\/jats:italic>.05). The percentage of pronuclear formation and cleaved embryos was increased (<jats:italic>p\u00a0<\/jats:italic>\u2264<jats:italic>\u00a0<\/jats:italic>.05) after 1.0<jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>e treatment (49.8\u00a0\u00b1\u00a02.8; 45.9\u00a0\u00b1\u00a01.5) compared to 0<jats:styled-content style=\"fixed-case\">mM<\/jats:styled-content>(41.4\u00a0\u00b1\u00a03.3; 38.9\u00a0\u00b1\u00a00.5) whereas the number of sperm binding\/oocyte did not significantly differ among groups. In summary, we suggest that<jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>e activated the velocity of Indochinese leopard sperm motility that may lead to faster sperm\/oocyte binding and sperm penetration (factors of successful embryo development).<\/jats:p>","DOI":"10.1111\/rda.12854","type":"journal-article","created":{"date-parts":[[2016,11,23]],"date-time":"2016-11-23T04:06:47Z","timestamp":1479874007000},"page":"269-274","source":"Crossref","is-referenced-by-count":4,"title":["Beneficial effect of extracellular adenosine 5\u2032\u2010triphosphate treatment on the Indochinese leopard (<i>Panthera pardus delacouri<\/i>) sperm quality after cryopreservation"],"prefix":"10.1111","volume":"52","author":[{"given":"P","family":"Thuwanut","sequence":"first","affiliation":[{"name":"Department of Obstetrics, Gynaecology and Reproduction Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Tipkantha","sequence":"additional","affiliation":[{"name":"Bureau of Conservation Research and Education Zoological Park Organization (ZPO) Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Siriaroonrat","sequence":"additional","affiliation":[{"name":"Bureau of Conservation Research and Education Zoological Park Organization (ZPO) Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Comizzoli","sequence":"additional","affiliation":[{"name":"Smithsonian Conservation Biology Institute National Zoological Park Washington DC USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Chatdarong","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, Gynaecology and Reproduction Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,11,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2003.11.003"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/1933719107306227"},{"key":"e_1_2_8_4_1","unstructured":"Henschel P. 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