{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T14:44:51Z","timestamp":1783694691320,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"S2","license":[{"start":{"date-parts":[[2016,11,3]],"date-time":"2016-11-03T00:00:00Z","timestamp":1478131200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Sperm cryopreservation offers a long\u2010term preservation of male genetic materials for future assisted reproductive technologies. However, dramatic changes in temperature during freezing and thawing injure sperm cells. While motility is essential for <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content> and membrane integrity is crucial for in vitro fertilization (<jats:styled-content style=\"fixed-case\">IVF<\/jats:styled-content>), sperm <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> integrity is a common index of fertilizing capability required for <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> IVF<\/jats:styled-content> and intracytoplasmic sperm injection (<jats:styled-content style=\"fixed-case\">ICSI<\/jats:styled-content>). In endangered felids died unexpectedly, attempts have been made to recover as many <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> intact spermatozoa as possible from epididymis and testis to increase the opportunity to produce offspring in future. Although sperm from caudal epididymis has shown retained fertilizing capability after freezing and thawing (27.3% conception rate after unilateral intrauterine insemination), sperm recovery from the corpus epididymis has been suggested as an alternative to increase the amount of preserved genetic materials. To improve epididymal sperm quality, pre\u2010treatment with single\u2010layer centrifugation resulted in selection of sperm cells with intact <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> while post\u2010thaw treatment with extracellular <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content> incubation promoted the blastocyst rate. Cold storage of domestic cat testis for 7\u00a0days at 4\u00b0C demonstrated &lt;1% of sperm cells with fragmented <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. Moreover, isolated testicular sperm cells, stored for 7\u00a0days at 4\u00b0C, produced after <jats:styled-content style=\"fixed-case\">ICSI<\/jats:styled-content> poorer percentages of cleavage, morula and blastocyst than the fresh control. In wild felids, a death\u2010to\u2010necropsy time of 2\u00a0hr after a jungle cat (<jats:italic>Felis chaus<\/jats:italic>) aged 10\u00a0years died during anaesthesia plus another necropsy\u2010to\u2010sperm recovery time of 25\u00a0hr has been reported to yield the post\u2010thawed testicular sperm with 22.2% intact <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. In summary, the chromatin structure of feline ejaculated and epididymal sperm seems to be tolerated to cold storage and cryopreservation; thus, fertilizing capability is well protected. In contrast, the cat testicular sperm <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> is generally damaged through the cryopreservation.<\/jats:p>","DOI":"10.1111\/rda.12855","type":"journal-article","created":{"date-parts":[[2016,11,3]],"date-time":"2016-11-03T06:38:45Z","timestamp":1478155125000},"page":"261-264","source":"Crossref","is-referenced-by-count":13,"title":["Retained fertilizing capability in cryopreserved feline spermatozoa"],"prefix":"10.1111","volume":"52","author":[{"given":"K","family":"Chatdarong","sequence":"first","affiliation":[{"name":"Department of Obstetrics, Gynaecology and Reproduction Faculty of Veterinary Science Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,11,3]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2003.11.003"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2007.00780.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)00170-3"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2012.03.011"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.09.022"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199414000276"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.07.022"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01218.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.12.009"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.09.030"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-12-0125"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD08226"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.081034"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2015.08.005"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.09.032"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12038"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.08.005"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.02.020"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/cryo.2000.2233"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.1.135"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.10-0400"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.03.005"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.110.085639"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh127"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.05.003"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.01.024"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.01.007"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.04.005"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2013.07.002"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.07.020"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.65.397"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.06.010"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X12469368"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.01.054"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.06.008"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.11.012"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00654.x"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12855","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12855","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12855","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T07:47:12Z","timestamp":1694591232000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12855"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,3]]},"references-count":37,"journal-issue":{"issue":"S2","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["10.1111\/rda.12855"],"URL":"https:\/\/doi.org\/10.1111\/rda.12855","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,3]]}}}