{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T20:21:40Z","timestamp":1788553300580,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,12,14]],"date-time":"2016-12-14T00:00:00Z","timestamp":1481673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007404","name":"Suomen El\u00e4inl\u00e4\u00e4ketieteen S\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007404","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006515","name":"El\u00e4inl\u00e4\u00e4ketieteen Tutkimuksen Tukis\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006515","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The aim of the study was to localize oxytocin receptors (<jats:styled-content style=\"fixed-case\">OTR<\/jats:styled-content>) and measure<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>expression of<jats:styled-content style=\"fixed-case\">OTR<\/jats:styled-content>in the canine uterus with and without the influence of progesterone. Uterine samples were taken from nine anoestrous and eight dioestrous bitches during ovariohysterectomy. Histological changes were evaluated in haematoxylin and eosin (<jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content>)\u2010stained samples. Purified polyclonal antibody for<jats:styled-content style=\"fixed-case\">OTR<\/jats:styled-content>was used in immunohistochemistry to localize receptors in uterine layers. Relative<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>concentration of<jats:styled-content style=\"fixed-case\">OTR<\/jats:styled-content>was evaluated with real\u2010time<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>from full\u2010thickness uterine samples taken from the middle horn and the body. Myometrial smooth muscle cells, endometrial luminal epithelium (<jats:styled-content style=\"fixed-case\">LE<\/jats:styled-content>) and deep and superficial glandular epithelium were positively stained for oxytocin receptors in non\u2010pregnant animals. No significant difference in staining intensity was detected between uterine middle horn and body. However, the staining intensity of<jats:styled-content style=\"fixed-case\">LE<\/jats:styled-content>was significantly higher in dioestrous than in anoestrous uteri (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Leucocytes and endothelium of blood vessels were also positively stained for<jats:styled-content style=\"fixed-case\">OTR<\/jats:styled-content>. Real\u2010time<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>showed no significant differences in<jats:styled-content style=\"fixed-case\">OTR mRNA<\/jats:styled-content>expression between the middle horn and the body of the uterus, or between anoestrous and dioestrous uterus. No correlation was noted between<jats:styled-content style=\"fixed-case\">OTR mRNA<\/jats:styled-content>expression and blood progesterone concentration. In conclusion, despite the apparent inactivity, the uterus of the non\u2010pregnant bitch expresses<jats:styled-content style=\"fixed-case\">OTR<\/jats:styled-content>. The distribution or relative expression of<jats:styled-content style=\"fixed-case\">OTR<\/jats:styled-content>does not differ between uterine horn and body in dioestrus or anoestrus except in<jats:styled-content style=\"fixed-case\">LE<\/jats:styled-content>.<jats:styled-content style=\"fixed-case\">LE<\/jats:styled-content>may have more oxytocin\u2010dependent activity during dioestrus than anoestrus.<\/jats:p>","DOI":"10.1111\/rda.12873","type":"journal-article","created":{"date-parts":[[2016,12,15]],"date-time":"2016-12-15T12:54:02Z","timestamp":1481806442000},"page":"153-159","source":"Crossref","is-referenced-by-count":3,"title":["Oxytocin receptors in dioestrous and anoestrous canine uteri"],"prefix":"10.1111","volume":"52","author":[{"given":"TM","family":"Tamminen","sequence":"first","affiliation":[{"name":"Department of Production Animal Medicine University of Helsinki Saarentaus Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Sahlin","sequence":"additional","affiliation":[{"name":"Department of Women's and Children's Health Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Masironi","sequence":"additional","affiliation":[{"name":"Department of Women's and Children's Health Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Taponen","sequence":"additional","affiliation":[{"name":"Department of Production Animal Medicine University of Helsinki Saarentaus Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O","family":"Laitinen\u2010Vapaavuori","sequence":"additional","affiliation":[{"name":"Department of Equine and Small Animal Medicine University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Katila","sequence":"additional","affiliation":[{"name":"Department of Production Animal Medicine University of Helsinki Saarentaus Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,12,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.1000469"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.domaniend.2006.06.005"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2006.03.029"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0007-1935(17)36935-X"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod13.1.112"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12015"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.2001.81.2.629"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.06.023"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12295"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0960837"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0029-1211280"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod56.3.632"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1023925906199"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2004.10.005"},{"key":"e_1_2_8_16_1","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/S0022-3565(25)38790-2","article-title":"Oxytocin causes endothelium\u2010dependent relaxations of canine basilar arteries by activating V1\u2010vasopressinergic receptors","volume":"236","author":"Katusic Z. 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