{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T14:40:13Z","timestamp":1720708813750},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,11,15]],"date-time":"2016-11-15T00:00:00Z","timestamp":1479168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The objective of the present study was to investigate the effect of testicular tissue lysate (<jats:styled-content style=\"fixed-case\">TTL<\/jats:styled-content>) on developmental competence of germinal vesicle (<jats:styled-content style=\"fixed-case\">GV<\/jats:styled-content>) stage porcine oocytes. Two types of <jats:styled-content style=\"fixed-case\">TTL<\/jats:styled-content> were prepared through repeated freeze\u2013thaw in liquid nitrogen, one from whole testicular tissue (<jats:styled-content style=\"fixed-case\">wTTL<\/jats:styled-content>) and other from either of four different sections of testes, namely just beneath the tunica albuginea (<jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content>), from the transitional area between the seminiferous cord\/tubules and the mediastinum testis (<jats:styled-content style=\"fixed-case\">TR<\/jats:styled-content>) and from the intermediate area (parenchymal tissue origin) and CE (cauda epididymis origin). The whole or section\u2010wise <jats:styled-content style=\"fixed-case\">TTL<\/jats:styled-content> treatments were given for 44\u00a0hr during in vitro maturation (<jats:styled-content style=\"fixed-case\">IVM<\/jats:styled-content>). Oocyte maturation was done in either of the two media, namely defined <jats:styled-content style=\"fixed-case\">(high\u2010performance basic medium for porcine oocyte maturation, commercially available)<\/jats:styled-content> and serum containing (<jats:styled-content style=\"fixed-case\">TCM<\/jats:styled-content>199). After maturation, oocytes were co\u2010incubated with fresh spermatozoa for 6\u00a0hr and then transferred to embryo culture media. Treatment of <jats:styled-content style=\"fixed-case\">GV<\/jats:styled-content> stage oocytes with <jats:styled-content style=\"fixed-case\">wTTL<\/jats:styled-content> (1\u00a0mg\/ml) increased the cleavage and morula percentage rate (69.23\u00a0\u00b1\u00a06.23 and 48.15\u00a0\u00b1\u00a06.77, respectively) than that of their control (58.33\u00a0\u00b1\u00a08.08 and 32.54\u00a0\u00b1\u00a05.53, respectively) in defined media, and in serum\u2010containing media, cleavage and morula percentage rate were almost equal in both treatment (54.56\u00a0\u00b1\u00a07.79 and 34.70\u00a0\u00b1\u00a06.78, respectively) and control (59.52\u00a0\u00b1\u00a08.21 and 38.52\u00a0\u00b1\u00a06.54, respectively). However, effect of <jats:styled-content style=\"fixed-case\">wTTL<\/jats:styled-content> was not significant. In case of section\u2010wise <jats:styled-content style=\"fixed-case\">TTL<\/jats:styled-content> supplements, <jats:styled-content style=\"fixed-case\">TR<\/jats:styled-content> section significantly (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01) improved cleavage and morula rate (58.43\u00a0\u00b1\u00a07.98 and 36.14\u00a0\u00b1\u00a06.89, respectively) followed by <jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content>. In conclusion, present study indicates that <jats:styled-content style=\"fixed-case\">IVM<\/jats:styled-content>, in vitro fertilization and in vitro culture of embryo are improved in the presence of <jats:styled-content style=\"fixed-case\">TTL<\/jats:styled-content>, particularly its <jats:styled-content style=\"fixed-case\">TR<\/jats:styled-content> section. Further study is expected to reveal the principal components of <jats:styled-content style=\"fixed-case\">TTL<\/jats:styled-content> which may prove useful for IVM.<\/jats:p>","DOI":"10.1111\/rda.12875","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T19:28:12Z","timestamp":1479324492000},"page":"183-188","source":"Crossref","is-referenced-by-count":1,"title":["Effect of testicular tissue lysate on developmental competence of porcine oocytes matured and fertilized in vitro"],"prefix":"10.1111","volume":"52","author":[{"given":"AK","family":"Singh","sequence":"first","affiliation":[{"name":"ICAR\u2010Indian Veterinary Research Institute  Bareilly Uttar Pradesh India"},{"name":"Department of Biotechnology Kumaun University  Nainital Uttarakhand India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Naskar","sequence":"additional","affiliation":[{"name":"ICAR\u2010National Research Centre on Pig  Guwahati Assam 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