{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T10:26:24Z","timestamp":1774607184633,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,12,7]],"date-time":"2016-12-07T00:00:00Z","timestamp":1481068800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2006\/05736\u20101"],"award-info":[{"award-number":["2006\/05736\u20101"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The maintaining of the epididymis at lower temperatures during storage and transport improves sperm quality. Our study aimed to test whether epididymis storage temperature (post\u2010mortem) and sperm cryopreservation affect sperm kinetics, membrane integrity, mitochondrial potential and fertility capacity. Thirty\u2010six epididymides were collected from 18 bulls after slaughter and divided into two groups: at 4 or 34\u00b0C for 2\u20133\u00a0hr. The sperm was collected from the epididymis cauda. The evaluation consisted of computer\u2010assisted sperm analysis (<jats:styled-content style=\"fixed-case\">CASA<\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">SYBR<\/jats:styled-content>14\/<jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">JC<\/jats:styled-content>1 to evaluate membrane integrity, mitochondrial membrane potential (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>) and measurement of lipid peroxidation (<jats:styled-content style=\"fixed-case\">TBARS<\/jats:styled-content>). The sperm was then frozen using an automatic device. After thawing, sperm samples were evaluated by the same variables and further <jats:italic>in vitro<\/jats:italic> fertilization rates. Cryopreservation negatively affected sperm motility in samples stored at 4 and 34\u00b0C. Nevertheless, the 4\u00b0C samples yielded higher rates of blastocyst formation. Pre\u2010freeze sperm motility, progressive motility and velocity were higher in sperm from epididymis stored at 4\u00b0C while post\u2010thaw sperm motility, progressive motility and velocity remained the same among samples from epididymis stored at 4 or 34\u00b0C. However, with regard to the kinetic patterns, samples collected from epididymis stored at 34\u00b0C had lower values when compared to those stored at 4\u00b0C prior the cryopreservation process. Our results indicate that epididymis handling conditions after cryopreservation may affect sperm quality after thawing, especially due to compromised <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content> in sperm collected from epididymis stored at higher temperatures.<\/jats:p>","DOI":"10.1111\/rda.12888","type":"journal-article","created":{"date-parts":[[2016,12,7]],"date-time":"2016-12-07T12:58:33Z","timestamp":1481115513000},"page":"257-263","source":"Crossref","is-referenced-by-count":32,"title":["Evaluation of epididymis storage temperature and cryopreservation conditions for improved mitochondrial membrane potential, membrane integrity, sperm motility and <i>in vitro<\/i> fertilization in bovine epididymal sperm"],"prefix":"10.1111","volume":"52","author":[{"given":"M","family":"Nichi","sequence":"first","affiliation":[{"name":"University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Rijsselaere","sequence":"additional","affiliation":[{"name":"Ghent University  Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JDA","family":"Losano","sequence":"additional","affiliation":[{"name":"University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DSR","family":"Angrimani","sequence":"additional","affiliation":[{"name":"University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GKV","family":"Kawai","sequence":"additional","affiliation":[{"name":"University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"IGF","family":"Goovaerts","sequence":"additional","affiliation":[{"name":"University of Antwerp  Antwerp Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Van Soom","sequence":"additional","affiliation":[{"name":"Ghent University  Ghent Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"VH","family":"Barnabe","sequence":"additional","affiliation":[{"name":"University of Sao Paulo  Sao Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JBP","family":"De Clercq","sequence":"additional","affiliation":[{"name":"University of Antwerp  Antwerp Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PEJ","family":"Bols","sequence":"additional","affiliation":[{"name":"University of Antwerp  Antwerp Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,12,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/cryo.1998.2141"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2014.10.023"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(00)00400-3"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(78)52032-6"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)01207-4"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/30.3.6174566"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2000.tb03388.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(95)92372-G"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod53.2.276"},{"key":"e_1_2_8_11_1","first-page":"384","article-title":"Quality and freezability of epididymal semen from African buffalo (Syncerus caffer) under field conditions","volume":"55","author":"Gerber D.","year":"2001","journal-title":"Theriogenology"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2605.1998.00106.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.11.018"},{"key":"e_1_2_8_14_1","first-page":"58","article-title":"Influence of homologous seminal plasma on bovine epididymal semen frozen with Triladyl or AndroMed\u00ae","volume":"90","author":"Herold F. 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