{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T06:14:11Z","timestamp":1777616051555,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,1,6]],"date-time":"2017-01-06T00:00:00Z","timestamp":1483660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004740","name":"Fondazione del Monte di Bologna e Ravenna","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004740","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Stallion semen storage for artificial insemination is mainly based on liquid cooled storage. In many stallions this technique maintains sperm quality for an extended period of time (24\u201372\u00a0hr) at 7\u00b0C. While this technique is commonly used in the horse industry, there can be a decline in fertility in some stallions, due to an inability of their sperm to tolerate the cool storage process. The aim of the present work was to evaluate the effect of two natural antioxidants (epigallocatechin\u20103\u2010gallate (<jats:styled-content style=\"fixed-case\">EGCG<\/jats:styled-content>) at 20, 60 and 120\u00a0\u03bc<jats:sc>m<\/jats:sc> and green tea polyphenols, and <jats:italic>p<\/jats:italic> at .001, .01 and .1\u00a0mg\/ml) on some sperm parameters (sperm motility, viability\/acrosome integrity and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> quality) in extended semen immediately after its collection (T0) and after 2, 6, 24 and 48\u00a0hr of cool storage. Two ejaculates from three trotter stallions were analysed after 48\u00a0hr of storage at 4\u00b0C. No beneficial effect on the analysed parameters was observed: the two antioxidants were not able to improve sperm quality after 48\u00a0hr of storage. These results are in agreement with previous findings on the effect of different antioxidants reported by other researches, who have demonstrated that stallion semen keeps good antioxidant capacity after dilution for 24\u00a0hr. In conclusion, the positive effect exerted by antioxidant molecules in other species is not confirmed in the equine one.<\/jats:p>","DOI":"10.1111\/rda.12891","type":"journal-article","created":{"date-parts":[[2017,1,6]],"date-time":"2017-01-06T09:22:58Z","timestamp":1483694578000},"page":"270-277","source":"Crossref","is-referenced-by-count":9,"title":["Epigallocatechin\u20103\u2010gallate (<scp>EGCG<\/scp>) and green tea polyphenols do not improve stallion semen parameters during cooling at 4\u00b0C"],"prefix":"10.1111","volume":"52","author":[{"given":"D","family":"Bucci","sequence":"first","affiliation":[{"name":"Department of Veterinary Medical Sciences Bologna University  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Spinaci","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences Bologna University  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Mislei","sequence":"additional","affiliation":[{"name":"National Institute for Artificial Insemination University of Bologna  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Gadani","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences Bologna University  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Rizzato","sequence":"additional","affiliation":[{"name":"National Institute for Artificial Insemination University of Bologna  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CC","family":"Love","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences College of Veterinary Medicine Texas A&amp;M University  College Station TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Tamanini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences Bologna University  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Galeati","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences Bologna University  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Mari","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medical Sciences Bologna University  Bologna Italy"},{"name":"National Institute for Artificial Insemination University of Bologna  Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,1,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.04.015"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(97)84066-6"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00828.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00590-8"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2001.62.508"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-008-9081-4"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2527\/2002.80112x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.110.011197"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.11.031"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2013.10.062"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0011-2240(77)90008-6"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2002.tb02599.x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2010.01609.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2014.09.011"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.06.032"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.07.005"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2005.06.019"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.5539\/jas.v5n8p231"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-08-0484"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0442.2006.00879.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12628"},{"key":"e_1_2_8_23_1","unstructured":"R Core Team(2015).R: A language and environment for statistical computing. 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