{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T22:58:18Z","timestamp":1790031498537,"version":"4.0.1"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,12,14]],"date-time":"2016-12-14T00:00:00Z","timestamp":1481673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"ICAR-National Dairy Research Institute, Karnal"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>This study compared endometrial cytology vis\u2010a\u2010vis uterine fluid cytology for assessment of uterine health in clinically normal and subclinical endometritis (SE)\u2010affected buffaloes. Uterine fluid samples and endometrial samples were collected from the buffaloes (<jats:italic>n<\/jats:italic>\u00a0=\u00a038) at oestrus using blue sheath and cytobrush, respectively. The smears were stained with Field stain for 3\u00a0minutes, and a minimum of 400 cells were counted in each smear for determination of the percentage of polymorphonuclear (PMN) leucocyte. The incidence of subclinical endometritis, based on the cytobrush cytology, was 23.08%. The correlation between cytobrush cytology with uterine fluid cytology was positive and significant (<jats:italic>r<\/jats:italic>\u00a0=\u00a0.37; <jats:italic>p<\/jats:italic>\u00a0=\u00a0.02). The ratio of PMN leucocyte in cytobrush cytology to uterine fluid cytology was 1:2.4. ROC analysis revealed that the threshold value of 6.16% PMN leucocyte in uterine fluid cytology showed a diagnostic sensitivity and specificity of 100% in differentiating normal from SE\u2010affected buffaloes. In conclusion, collection of uterine fluid was easier compared to collection of endometrial samples using cytobrush and the percentage of PMN leucocyte in uterine fluid cytology can be used as a tool for diagnosis of subclinical endometritis in buffaloes.<\/jats:p>","DOI":"10.1111\/rda.12899","type":"journal-article","created":{"date-parts":[[2016,12,15]],"date-time":"2016-12-15T12:39:53Z","timestamp":1481805593000},"page":"513-516","source":"Crossref","is-referenced-by-count":7,"title":["Efficiency of uterine fluid cytology in the diagnosis of subclinical endometritis in the water buffalo (<i>Bubalus bubalis<\/i>)"],"prefix":"10.1111","volume":"52","author":[{"given":"SC","family":"Gahlot","sequence":"first","affiliation":[{"name":"Animal Reproduction Gynaecology &amp; Obstetrics National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Kumar","sequence":"additional","affiliation":[{"name":"Animal Reproduction Gynaecology &amp; Obstetrics National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Kumaresan","sequence":"additional","affiliation":[{"name":"Animal Reproduction Gynaecology &amp; Obstetrics National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Chand","sequence":"additional","affiliation":[{"name":"Animal Reproduction Gynaecology &amp; Obstetrics National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RK","family":"Baithalu","sequence":"additional","affiliation":[{"name":"Animal Reproduction Gynaecology &amp; Obstetrics National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Lathika","sequence":"additional","affiliation":[{"name":"Animal Reproduction Gynaecology &amp; Obstetrics National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TK","family":"Patbandha","sequence":"additional","affiliation":[{"name":"Animal Reproduction Gynaecology &amp; Obstetrics National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SS","family":"Lathwal","sequence":"additional","affiliation":[{"name":"Animal Reproduction Gynaecology &amp; Obstetrics National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TK","family":"Mohanty","sequence":"additional","affiliation":[{"name":"Animal Reproduction Gynaecology &amp; Obstetrics National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,12,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.01.010"},{"key":"e_1_2_8_3_1","first-page":"154","article-title":"Uterine infection in buffalo: A review","volume":"29","author":"Azawi O. I.","year":"2010","journal-title":"Buffalo Bulletin"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.12.005"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2008-1615"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.48.371"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.04.022"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2006.03.022"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2003.03.001"},{"key":"e_1_2_8_10_1","first-page":"255","article-title":"A comparison of the cytobrush and uterine lavage techniques to evaluate endometrial cytology in clinically normal postpartum dairy cows","volume":"46","author":"Kasimanickam R.","year":"2005","journal-title":"Canadian Veterinary Journal"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1071\/AN14941"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.11.006"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(02)74302-6"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0531.2002.00317.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2006.03.017"},{"key":"e_1_2_8_16_1","first-page":"213","article-title":"Pathology of uterus in buffalo slaughtered in Ahwaz (Iran) abattoir","volume":"12","author":"Moghami S. M.","year":"1996","journal-title":"Buffalo Journal"},{"key":"e_1_2_8_17_1","first-page":"399","volume-title":"Arthur's veterinary reproduction and obstetrics","author":"Noakes D. E.","year":"2002"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(82)90162-5"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.05.033"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.08.021"},{"key":"e_1_2_8_21_1","unstructured":"Srivastava A. K. &Kumaresan A.(2014)Scope of buffaloes in Indian dairy industry.Asian Buffalo Magazine January\u2013June pp.16\u201327."},{"key":"e_1_2_8_22_1","first-page":"1","volume-title":"Status paper on \u201cBuffalo Estrus Biology\u201d","author":"Srivastava A. K.","year":"2013"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0258(19980430)17:8<857::AID-SIM777>3.0.CO;2-E"},{"key":"e_1_2_8_24_1","first-page":"154","article-title":"Improving conception rate in buffaloes (Bubalus bubalis): Management tools to improve estrus detection efficiency and accuracy","volume":"67","author":"Verma K. K.","year":"2014","journal-title":"Indian Journal of Dairy Science"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2014.467-471"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12899","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12899","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12899","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T22:56:23Z","timestamp":1696028183000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12899"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,14]]},"references-count":24,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["10.1111\/rda.12899"],"URL":"https:\/\/doi.org\/10.1111\/rda.12899","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,14]]}}}