{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T21:57:34Z","timestamp":1781819854381,"version":"3.54.5"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,2,7]],"date-time":"2017-02-07T00:00:00Z","timestamp":1486425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","award":["NFBSFARA\/4006"],"award-info":[{"award-number":["NFBSFARA\/4006"]}],"id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Assessment of urine levels of luteinizing hormone (LH) for predicting the reproductive status of animals is in practice. The aim of this study was to predict the period of ovulation based on the urine levels of LH for timed\u2010artificial insemination to increase the conception rate in buffaloes, which are naturally silent\u2010oestrous animals. Level of <jats:styled-content style=\"fixed-case\">LH<\/jats:styled-content> in urine was assessed using <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>, and a cut\u2010off <jats:styled-content style=\"fixed-case\">LH<\/jats:styled-content> concentration for prediction of ovulation period was obtained using receiver operating characteristic analysis. Artificial insemination was performed before\u2010 and after \u2010positive prediction of ovulation period adopting this method, and the rates of conception were assessed. Urine <jats:styled-content style=\"fixed-case\">LH<\/jats:styled-content> level of 105\u00a0mIU\/ml (<jats:italic>n<\/jats:italic>\u00a0=\u00a014) was derived as a cut\u2010off concentration which predicts the ovulation period. The buffaloes in the positively predicted group (day 1 or 2) inseminated via intracervical route had an increase in the conception rate (83.33%); however, the insemination in the before\u2010positive\u2010prediction group resulted in poor conception rates (day 0; 16.66%) compared to that of the naturally inseminated group (day 0; 75.0%). In conclusion, the urinary <jats:styled-content style=\"fixed-case\">LH<\/jats:styled-content> would possibly be a fairly reliable predictor of the ovulation period. The day when cut\u2010off <jats:styled-content style=\"fixed-case\">LH<\/jats:styled-content> concentration is obtained may be taken as the most favourable time for artificial insemination, so as to attain a much better rate of conception in the buffalo.<\/jats:p>","DOI":"10.1111\/rda.12933","type":"journal-article","created":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T04:46:56Z","timestamp":1486529216000},"page":"517-521","source":"Crossref","is-referenced-by-count":4,"title":["Urine levels of luteinizing hormone as predictor of the period of ovulation for advantage of timed\u2010artificial insemination in murrah buffalo (<i>Bubalus bubalis<\/i>)"],"prefix":"10.1111","volume":"52","author":[{"given":"RM","family":"Selvam","sequence":"first","affiliation":[{"name":"Centre for Pheromone Technology Department of Animal Science Bharathidasan\u00a0University  Tiruchirappalli India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Singh","sequence":"additional","affiliation":[{"name":"Molecular Endocrinology Laboratory Animal Biochemistry Division National Dairy Research Institute  Karnal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MA","family":"Akbarsha","sequence":"additional","affiliation":[{"name":"Centre for Pheromone Technology Department of Animal Science Bharathidasan\u00a0University  Tiruchirappalli India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Archunan","sequence":"additional","affiliation":[{"name":"Centre for Pheromone Technology Department of Animal Science Bharathidasan\u00a0University  Tiruchirappalli India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1201\/b16511-17"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1710131"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2006.08.009"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-7-8"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod33.2.316"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2345(1996)40:3<231::AID-AJP2>3.0.CO;2-Z"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(03)73790-4"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(86)90098-9"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dei098"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.5455\/vetworld.2013.59-63"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0029-7844(95)00352-5"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.2007.124"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.113.113852"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/13.4.826"},{"key":"e_1_2_9_16_1","unstructured":"Paul V. (2003).Studies on estrus synchronization methods timing of ovulation and endocrine profile in buffaloes. Ph.D. thesis National Dairy Research Institute Karnal Haryana India."},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2010.08.022"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2003.07.004"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00516"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2016.12.002"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12933","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12933","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T22:56:12Z","timestamp":1696028172000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12933"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,7]]},"references-count":19,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["10.1111\/rda.12933"],"URL":"https:\/\/doi.org\/10.1111\/rda.12933","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,7]]}}}