{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T06:27:29Z","timestamp":1762324049207,"version":"3.37.3"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T00:00:00Z","timestamp":1486512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Program for Chang Jiang Scholars and Innovative Research Team in University","award":["IRT13091"],"award-info":[{"award-number":["IRT13091"]}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"crossref","award":["31920160012"],"award-info":[{"award-number":["31920160012"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,6]]},"abstract":"Contents<\/jats:title>Follicle\u2010stimulating hormone (FSH<\/jats:styled-content>) and luteinizing hormone (LH<\/jats:styled-content>) have a central role in follicle growth, maturation and oestrus, but no clear pathway in the seasonal oestrus of yak (Bos grunniens<\/jats:italic>) has been found. To better understand the role of FSH<\/jats:styled-content> and LH<\/jats:styled-content> in seasonal oestrus in the yak, six yaks were slaughtered while in oestrus, and the pineal gland, hypothalamus, pituitary gland, and gonads were collected. Using real\u2010time PCR<\/jats:styled-content> and immunohistochemical assays, we determined the mRNA<\/jats:styled-content> and protein expression of the FSH<\/jats:styled-content> and LH<\/jats:styled-content> receptors (FSHR<\/jats:styled-content> and LHR<\/jats:styled-content>) in these organs. The analysis showed that the FSHR mRNA<\/jats:styled-content> expression level was higher in the pituitary gland tissue compared with LHR<\/jats:styled-content> (p\u00a0<\/jats:italic><\u00a0<\/jats:italic>.01) during oestrus. By contrast, there was low expression of FSHR<\/jats:styled-content> and LHR mRNA<\/jats:styled-content> in the pineal gland and hypothalamus. FSHR mRNA<\/jats:styled-content> expression was higher than that of LHR<\/jats:styled-content> (p\u00a0<\/jats:italic><\u00a0<\/jats:italic>.05) in the ovary, whereas LHR mRNA<\/jats:styled-content> expression was higher than that of FSHR<\/jats:styled-content> (p\u00a0<\/jats:italic><\u00a0<\/jats:italic>.01) in the uterus. FSHR<\/jats:styled-content> and LHR<\/jats:styled-content> proteins were located in the pinealocyte, synaptic ribbon and synaptic spherules of the pineal gland and that FSH<\/jats:styled-content> and LH<\/jats:styled-content> interact via nerve fibres. In the hypothalamus, FSHR<\/jats:styled-content> and LHR<\/jats:styled-content> proteins were located in the magnocellular neurons and parvocellular neurons. FSHR<\/jats:styled-content> and LHR<\/jats:styled-content> proteins were localized in acidophilic cells and basophilic cells in the pituitary gland, and in surface epithelium, stromal cell and gland epithelium in the uterus. In the ovary, FSHR<\/jats:styled-content> and LHR<\/jats:styled-content> protein were present in the ovarian follicle. Thus, we concluded that FSHR<\/jats:styled-content> and LHR<\/jats:styled-content> are located in the pineal gland, hypothalamus, pituitary and gonad during oestrus in the yak. However, FSHR<\/jats:styled-content> was mainly expressed in the pituitary gland and ovaries, whereas LHR<\/jats:styled-content> was mainly expressed in the pituitary gland and uterus.<\/jats:p>","DOI":"10.1111\/rda.12936","type":"journal-article","created":{"date-parts":[[2017,2,9]],"date-time":"2017-02-09T04:34:42Z","timestamp":1486614882000},"page":"477-482","source":"Crossref","is-referenced-by-count":11,"title":["Protein and mRNA<\/scp> expression of follicle\u2010stimulating hormone receptor and luteinizing hormone receptor during the oestrus in the yak (Bos grunniens<\/i>)"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0197-8097","authenticated-orcid":false,"given":"S\u2010d","family":"Huo","sequence":"first","affiliation":[{"name":"Animal Cell Engineering and Technology Research Center of Gansu Northwest University for Nationalities Lanzhou China"},{"name":"College of Life Science and Engineering Northwest University for Nationalities Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u2010e","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering Northwest University for Nationalities Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u2010j","family":"Long","sequence":"additional","affiliation":[{"name":"College of Life Science Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u2010t","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering Northwest University for Nationalities Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u2010x","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering Northwest University for Nationalities Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u2010x","family":"Zang","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering Northwest University for Nationalities Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T\u2010j","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering Northwest University for Nationalities Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Abudureyimu","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering Northwest University for Nationalities Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u2010l","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering Northwest University for Nationalities Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u2010h","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering Northwest University for Nationalities Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y\u2010q","family":"Zhao","sequence":"additional","affiliation":[{"name":"College of Life Science and Engineering Northwest University for Nationalities Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z\u2010r","family":"Ma","sequence":"additional","affiliation":[{"name":"Animal Cell Engineering and Technology Research Center of Gansu Northwest University for Nationalities Lanzhou China"},{"name":"College of Life Science and Engineering Northwest University for Nationalities Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.03.019"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2012.05.013"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/mgme.1997.2650"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2006.04.015"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.17221\/7294-CJAS"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1806-92902015001000002"},{"key":"e_1_2_8_8_1","first-page":"39","article-title":"Control of follicular growth and development","volume":"34","author":"Ireland J. 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