{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T13:48:18Z","timestamp":1762004898331,"version":"3.37.3"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,3,29]],"date-time":"2017-03-29T00:00:00Z","timestamp":1490745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>In this study, sperms collected from the right and left cauda epididymis were grouped into having canine prostatic fluid (<jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content>) sensitization or not diluted with egg yolk Tris\u2013fructose citrate extender, and stored at 4\u00b0C. The necessity of canine <jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content> in cooled preservation was determined by elucidating the sperm quality after the storage. As a result, while there was no difference among all groups up to 48\u00a0hr of storage, after storage for 96\u00a0hr and more, a significantly lower sperm motility was observed in the group without being sensitized to <jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content> than the groups with being sensitized to <jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content> (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01). Although sperm abnormality increased in all groups with increased storage time, the group without being sensitized to <jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content> showed significantly higher sperm abnormality than did the groups with being sensitized to <jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content> after storage for 24\u00a0hr and more (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01). From these findings, we concluded that <jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content> was necessary for the cooled preservation of the canine sperm because these sperms were protected from any effects of low temperatures by being sensitized to <jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/rda.12963","type":"journal-article","created":{"date-parts":[[2017,3,29]],"date-time":"2017-03-29T07:34:13Z","timestamp":1490772853000},"page":"655-660","source":"Crossref","is-referenced-by-count":2,"title":["Role of prostatic fluid in cooled canine epididymal sperm"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4996-6327","authenticated-orcid":false,"given":"T","family":"Hori","sequence":"first","affiliation":[{"name":"Laboratory of Reproduction Nippon Veterinary and Life Science University  Musashino\u2010shi Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Masuda","sequence":"additional","affiliation":[{"name":"Laboratory of Reproduction Nippon Veterinary and Life Science University  Musashino\u2010shi Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Laboratory of Reproduction Nippon Veterinary and Life Science University  Musashino\u2010shi Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Kawakami","sequence":"additional","affiliation":[{"name":"Laboratory of Reproduction Nippon Veterinary and Life Science University  Musashino\u2010shi Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,3,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2005.12.026"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/179258a0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(96)00151-3"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2012.08.032"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.14-0421"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.67.1141"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.05.027"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.66.37"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.71.811"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.64.543"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.07.015"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0195-5616(91)50054-1"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)00222-8"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2006.00766.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)00663-5"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD01113"},{"key":"e_1_2_8_18_1","first-page":"383","article-title":"Effect of prostatic fluid on motility, viability and acrosome integrity of chilled and frozen\u2010thawed dog spermatozoa","volume":"57","author":"Sirivaidyapong S.","year":"2001","journal-title":"Journal of Reproduction and Fertility. 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