{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T00:41:25Z","timestamp":1789346485422,"version":"build-2803163510"},"reference-count":60,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,4,22]],"date-time":"2017-04-22T00:00:00Z","timestamp":1492819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004607","name":"Natural Science Foundation of Guangxi Province","doi-asserted-by":"publisher","award":["2014GXNSFCB118003"],"award-info":[{"award-number":["2014GXNSFCB118003"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004607","name":"Natural Science Foundation of Guangxi Province","doi-asserted-by":"publisher","award":["2016GXNSFCA380007"],"award-info":[{"award-number":["2016GXNSFCA380007"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,10]]},"abstract":"Contents<\/jats:title>Histone deacetylase inhibitors (HDAC<\/jats:styled-content>is) can change the histone acetylation and significantly enhance the developmental competence of the pre\u2010implantation SCNT<\/jats:styled-content> embryo. To select a proper histone deacetylase inhibitor to improve the success rate and potentially developmental ability of handmade cloning (HMC<\/jats:styled-content>) embryos of miniature porcine, we compared the effect of two histone deacetylase inhibitors (SAHA<\/jats:styled-content> vs. VPA<\/jats:styled-content>) on HMC<\/jats:styled-content> embryo development, their histone acetylation level and the expression level of relevant genes. The blastocyst rate and number of blastocyst cells of HMC<\/jats:styled-content> embryos treated with SAHA<\/jats:styled-content> (SAHA<\/jats:styled-content>\u2010HMC<\/jats:styled-content>) or VPA<\/jats:styled-content> (VPA<\/jats:styled-content>\u2010HMC<\/jats:styled-content>) were significantly higher than those of control (Control\u2010HMC<\/jats:styled-content>), respectively, but there were no significant difference between SAHA<\/jats:styled-content>\u2010HMC<\/jats:styled-content> and VPA<\/jats:styled-content>\u2010HMC<\/jats:styled-content> groups. In addition, the acetylation level (AcH4K8) of Control\u2010HMC<\/jats:styled-content> and VPA<\/jats:styled-content>\u2010HMC<\/jats:styled-content> embryos at the blastocyst stage, respectively, was significantly lower than that of in vitro fertilized (IVF<\/jats:styled-content>) and SAHA<\/jats:styled-content>\u2010HMC<\/jats:styled-content> embryos. However, the acetylation H4K8 of the blastocysts had no significant difference between SAHA<\/jats:styled-content>\u2010HMC<\/jats:styled-content> and the IVF<\/jats:styled-content> groups. The SAHA<\/jats:styled-content>\u2010HMC<\/jats:styled-content> blastocysts indicated comparative expression levels of Oct4 and HDAC<\/jats:styled-content>1 (histone deacetyltransferase gene) with those of IVF<\/jats:styled-content> blastocysts. In contrast, the expression levels of Oct4 were lower and those of HDAC<\/jats:styled-content>1 were higher in the VPA<\/jats:styled-content>\u2010HMC<\/jats:styled-content> and Control\u2010HMC<\/jats:styled-content> blastocysts, respectively, compared to those of the IVF<\/jats:styled-content> blastocysts. Our results demonstrated that the HMC<\/jats:styled-content> embryos treated by SAHA<\/jats:styled-content> could promote the pre\u2010implantation development and increase the levels of histone H4K8 acetylation and the expression of the OCT<\/jats:styled-content>4 gene, yet decrease the expression of the HDAC<\/jats:styled-content>1 gene to the comparable level of the IVF<\/jats:styled-content> embryos. Our results proved that SAHA<\/jats:styled-content> may be a better histone deacetylase inhibitor for porcine HMC<\/jats:styled-content> compared to VPA<\/jats:styled-content>, and furthermore, it may indicate that SAHA<\/jats:styled-content> can effectively correct the abnormal histone acetylation during the HMC<\/jats:styled-content> embryo development and subsequently improve the full\u2010term developmental potential of the HMC<\/jats:styled-content> embryos after embryo transplantation.<\/jats:p>","DOI":"10.1111\/rda.12977","type":"journal-article","created":{"date-parts":[[2017,4,22]],"date-time":"2017-04-22T07:46:53Z","timestamp":1492847213000},"page":"763-774","source":"Crossref","is-referenced-by-count":13,"title":["Suberoylanilide hydroxamic acid, a novel histone deacetylase inhibitor, improves the development and acetylation level of miniature porcine handmade cloning embryos"],"prefix":"10.1111","volume":"52","author":[{"given":"JM","family":"Sun","sequence":"first","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro\u2010bioresources Guangxi University Nanning Guangxi China"},{"name":"Guangxi High Education Laboratory for Animal Reproduction and Biotechnology Guangxi University Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KQ","family":"Cui","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro\u2010bioresources Guangxi University Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ZP","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro\u2010bioresources Guangxi University Nanning Guangxi China"},{"name":"Guangxi High Education Laboratory for Animal Reproduction and Biotechnology Guangxi University Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XR","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro\u2010bioresources Guangxi University Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ZF","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro\u2010bioresources Guangxi University Nanning Guangxi China"},{"name":"Guangxi High Education Laboratory for Animal Reproduction and Biotechnology Guangxi University Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"QY","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro\u2010bioresources Guangxi University Nanning Guangxi China"},{"name":"Guangxi High Education Laboratory for Animal Reproduction and Biotechnology Guangxi University Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7223-3984","authenticated-orcid":false,"given":"B","family":"Huang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro\u2010bioresources Guangxi University Nanning Guangxi China"},{"name":"Guangxi High Education Laboratory for Animal Reproduction and Biotechnology Guangxi University Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DS","family":"Shi","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro\u2010bioresources Guangxi University Nanning Guangxi China"},{"name":"Guangxi High Education Laboratory for Animal Reproduction and Biotechnology Guangxi University Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)01077-2"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20618"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2009.06.030"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-10-0455"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj20021321"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.241522698"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.07.021"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/clo.2005.7.199"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.017954"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/43710"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.24.6969"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/38664"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.05.042"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1418"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/clo.2007.0002"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.295.5553.297"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/88903"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.08.033"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod63.6.1698"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.11.164"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.03.021"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD04007"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21042"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90051-Q"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.04.017"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg887"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.106.052514"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.05.046"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.07.013"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1089\/cell.2009.0032"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81769-9"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1089\/153623002753632020"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.110.085282"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210602"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2011.07.006"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/35024082"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.6.3003"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(03)00419-6"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2007-0718"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.10.026"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD12336"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1089\/cell.2011.0099"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/47412"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD03052"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)01080-2"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.02.010"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD04116"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD06089"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-08-0299"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.107.063149"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.10.022"},{"key":"e_1_2_9_53_1","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1089\/cell.2011.0024","article-title":"Scriptaid improves in vitro development and nuclear reprogramming of somatic cell nuclear transfer bovine embryos","volume":"13","author":"Wang L.\u2010J.","year":"2011","journal-title":"Cellular Reprogramming"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M511340200"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.02.004"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1089\/cell.2011.0084"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09229"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.20245"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1089\/clo.2006.0090"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.077016"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2010.11.015"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.12977","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.12977","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T23:27:03Z","timestamp":1696030023000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.12977"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,22]]},"references-count":60,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,10]]}},"alternative-id":["10.1111\/rda.12977"],"URL":"https:\/\/doi.org\/10.1111\/rda.12977","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,22]]}}}