{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T05:53:54Z","timestamp":1759730034723,"version":"3.37.3"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,6,5]],"date-time":"2017-06-05T00:00:00Z","timestamp":1496620800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Among others, selenium (Se) and vitamin E (VitE) have been provided to dogs to improve semen quality. However, scientific evidence documenting an effect in dogs is lacking. The aim of this study was to investigate the effect of supplementation of these antioxidants on various ejaculate parameters in a randomized, double\u2010blinded trial using Cairn Terrier males exhibiting normal seminal quality parameters. Three dogs each were fed a standardized diet and supplemented with 0.1\u00a0mg Se, 100\u00a0mg VitE or 0.1\u00a0mg Se\u00a0+\u00a0100\u00a0mg VitE\/dog for 3\u00a0months. Ejaculate analyses (volume, progressive motility, vitality, morphology, concentration) were performed before inclusion (D0) and after 1, 2 and 3\u00a0months (+1, +2, +3). At the same time, glutathione peroxidase (GSH\u2010PX) and VitE in seminal plasma (SP) and GSH\u2010PX in blood samples were determined. Vitamin E levels in SP were below the detection limit (1.0\u00a0mg\/L) in all samples. GSH\u2010PX in blood (164.0\u20132794.4\u00a0IU\/L) and SP (18.4\u20134326.0\u00a0IU\/L) was highly variable. Supplementation only significantly affected the total percentage of sperm head abnormalities (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.011). Time significantly affected the percentage of morphologically abnormal sperm (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.025), sperm head abnormalities (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.007), proximal droplets (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.001) and GSH\u2010PX in SP (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.015). Additionally, a significant interaction between time and group was identified for the percentage of membrane\u2010intact sperm (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.048), head abnormalities (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.018), acrosomal defects (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.043) and proximal droplets (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.002). Although some effects could be identified for selected parameters, we failed to identify a clear trend about how a 3\u00a0months VitE and\/or Se supplementation affects semen parameters in normospermic Cairn Terriers.<\/jats:p>","DOI":"10.1111\/rda.13000","type":"journal-article","created":{"date-parts":[[2017,6,5]],"date-time":"2017-06-05T10:43:16Z","timestamp":1496659396000},"page":"945-952","source":"Crossref","is-referenced-by-count":16,"title":["Effect of dietary vitamin E and selenium supplementation on semen quality in Cairn Terriers with normospermia"],"prefix":"10.1111","volume":"52","author":[{"given":"KT","family":"Kirchhoff","sequence":"first","affiliation":[{"name":"Dyrl\u00e6gegruppen Frijsenborg A\/S  Hammel Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Failing","sequence":"additional","affiliation":[{"name":"Unit for Biomathematics and Data Processing Justus\u2010Liebig\u2010University Giessen  Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4327-9780","authenticated-orcid":false,"given":"S","family":"Goericke\u2010Pesch","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Science Section for Veterinary Reproduction and Obstetrics Faculty of Health and Medical Sciences University of Copenhagen  Frederiksberg C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,6,5]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"158","article-title":"Pharmacology of free radicals and the impact of reactive oxygen species on the testis","volume":"14","author":"Aprioku J. 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