{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T10:11:36Z","timestamp":1771841496431,"version":"3.50.1"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,6,28]],"date-time":"2017-06-28T00:00:00Z","timestamp":1498608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004681","name":"Higher Education Commission, Pakistan","doi-asserted-by":"publisher","award":["PM\u2010IPFP\/HRD\/HEC\/2011\/368"],"award-info":[{"award-number":["PM\u2010IPFP\/HRD\/HEC\/2011\/368"]}],"id":[{"id":"10.13039\/501100004681","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The study was designed to evaluate AndroMed<jats:sup>\u00ae<\/jats:sup> for the freezability and fertility of Nili\u2010Ravi buffalo semen. Semen was collected from four adult Nili\u2010Ravi buffalo (<jats:italic>Bubalus bubalis<\/jats:italic>) bulls for 3\u00a0weeks (replicate). Semen ejaculates from each buffalo bull were divided into three aliquots. One aliquot was used for evaluation of motility, plasma membrane integrity, livability, viability, <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> integrity and normal apical ridge. Remaining two aliquots were diluted (37\u00b0C; 50\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup> spermatozoa\/ml) in <jats:italic>tris<\/jats:italic>\u2010citric egg yolk or AndroMed<jats:sup>\u00ae<\/jats:sup> extender and cryopreserved in 0.5\u00a0ml French straws. After thawing, per cent post\u2010thaw motility (47.9\u00a0\u00b1\u00a00.8, 49.2\u00a0\u00b1\u00a01.7), plasma membrane integrity (44.4\u00a0\u00b1\u00a01.2, 46.8\u00a0\u00b1\u00a01.8) and normal apical ridge (81.4\u00a0\u00b1\u00a00.3, 83.2\u00a0\u00b1\u00a00.3) were recorded similar (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05) in <jats:italic>tris<\/jats:italic>\u2010citric egg yolk and AndroMed<jats:sup>\u00ae<\/jats:sup> extender. Higher (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) percentage of sperm livability (70.5\u00a0\u00b1\u00a01.4 and 64.4\u00a0\u00b1\u00a01.0), viability (67.5\u00a0\u00b1\u00a01.5 and 61.5\u00a0\u00b1\u00a00.6) and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> integrity (97.0\u00a0\u00b1\u00a00.3 and 93.4\u00a0\u00b1\u00a00.21) were recorded in AndroMed<jats:sup>\u00ae<\/jats:sup> compared to tris\u2010citric egg yolk post\u2010thaw. Values for all the aforementioned spermatozoal quality parameters were observed lower (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) in frozen\u2010thawed compared to fresh semen irrespective of the experimental extenders. Fertility rates of buffalo semen did not differ (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05) either cryopreserved in <jats:italic>tris<\/jats:italic>\u2010citric egg yolk or AndroMed<jats:sup>\u00ae<\/jats:sup> extender (45.5% vs. 49%). It is concluded that AndroMed<jats:sup>\u00ae<\/jats:sup> is capable in protecting the buffalo bull sperm during freeze\u2010thawing process and can be adopted safely for routine use replacing the tris\u2010citric egg yolk extender in artificial insemination programme.<\/jats:p>","DOI":"10.1111\/rda.13008","type":"journal-article","created":{"date-parts":[[2017,6,29]],"date-time":"2017-06-29T04:26:41Z","timestamp":1498710401000},"page":"992-997","source":"Crossref","is-referenced-by-count":9,"title":["Cryopreservation of Nili\u2010Ravi buffalo (<i>Bubalus bubalis<\/i>) semen in AndroMed<sup>\u00ae<\/sup> extender; in vitro and in vivo evaluation"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6441-3953","authenticated-orcid":false,"given":"MS","family":"Ansari","sequence":"first","affiliation":[{"name":"Department of Zoology University of Sargodha Lyallpur Campus  Faislabad Pakistan"},{"name":"Department of Zoology University of Gujrat  Gujrat Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BA","family":"Rakha","sequence":"additional","affiliation":[{"name":"Department of Wildlife Management Pir Mehr Ali Shah Arid Agriculture University  Rawalpindi Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Akhter","sequence":"additional","affiliation":[{"name":"Department of Zoology Pir Mehr Ali Shah Arid Agriculture University  Rawalpindi Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,6,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)01369-9"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2011.01973.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2007.00890.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.04.024"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2009.01561.x"},{"issue":"2","key":"e_1_2_8_7_1","first-page":"131","article-title":"Comparison of egg yolks from three avian species in extender for cryopreservation of Sahiwal bull epididymal spermatozoa","volume":"29","author":"Akhter S.","year":"2010","journal-title":"Animal Science Papers and Reports"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.09.002"},{"issue":"1","key":"e_1_2_8_9_1","first-page":"13","article-title":"Effect of antibiotics in extender on fertility of liquid buffalo bull semen","volume":"27","author":"Akhter S.","year":"2007","journal-title":"Pakistan Veterinary Journal"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD12181"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00011"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.07.003"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.09.035"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1642-431X(12)60063-1"},{"issue":"6","key":"e_1_2_8_15_1","first-page":"741","article-title":"Cryopreservation of sahiwal bull epididymal spermatozoa","volume":"42","author":"Ansari M. S.","year":"2010","journal-title":"Pakistan Journal of Zoology"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.repbio.2012.10.001"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deg228"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(98)00175-7"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)01177-9"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)01178-0"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2006.12.010"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(03)00256-5"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.03.007"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1530\/jrf.0.0700219"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(91)78576-7"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520299209110020"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2015.05.010"},{"key":"e_1_2_8_28_1","unstructured":"Muller\u2010Schlosser F. Aires V. Hinsch E. &Hinsch K.D. (2001).Evaluation of the quality of a new generation of egg yolk free semen diluters for cryopreservation of bovine semen. Jahrestaung uber Physiologie und Pathologie der Fortpflanzung p.54."},{"key":"e_1_2_8_29_1","unstructured":"Nehring H. &Rothe L. (2003).Insemination of cryopreserved bull semen portions with reduced sperm numbers after dilution with two egg yolk\u2010free extenders. In Proceedings of the 15th European AI Vets Meeting Budapest Hungary pp.14\u201323."},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(40)95541-2"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00580-013-1708-6"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(79)83415-3"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.01.009"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.01.012"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(00)00161-5"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1071\/BI9750145"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-06-0256"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.13008","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T05:45:16Z","timestamp":1694324716000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.13008"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,28]]},"references-count":36,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1111\/rda.13008"],"URL":"https:\/\/doi.org\/10.1111\/rda.13008","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,28]]}}}