{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T17:57:21Z","timestamp":1759773441171,"version":"3.37.3"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,7,9]],"date-time":"2017-07-09T00:00:00Z","timestamp":1499558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005416","name":"Norges Forskningsr\u00e5d","doi-asserted-by":"publisher","award":["207799"],"award-info":[{"award-number":["207799"]}],"id":[{"id":"10.13039\/501100005416","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The SpermVital<jats:sup>\u00ae<\/jats:sup> technology comprises embedding of spermatozoa within an alginate gel to facilitate release of sperm cells over a prolonged period in utero after <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>. The aim of this study was to examine whether the survival time of spermatozoa is extended when applying this immobilization technology in combination with cryopreservation. Sperm cell survival (acrosome and plasma membrane integrity) was studied in vitro for 48\u00a0hr at physiological temperature. One dose of SpermVital<jats:sup>\u00ae<\/jats:sup> (<jats:styled-content style=\"fixed-case\">SV<\/jats:styled-content>) semen was compared with single doses of Biladyl<jats:sup>\u00ae<\/jats:sup> (B) processed semen as well as double doses of B (B double). B double was obtained by adding a second B dose the following day, thereby mimicking double <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>. Furthermore, reproductive performance applying single early timed <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">TAI<\/jats:styled-content>) with <jats:styled-content style=\"fixed-case\">SV<\/jats:styled-content> following oestrus synchronization was studied in a field trial. Double insemination (<jats:styled-content style=\"fixed-case\">TAI<\/jats:styled-content> on two consecutive days) with B semen served as control. Number of acrosome\u2010intact live sperm cells decreased over time in vitro for all treatments (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). There was no difference between <jats:styled-content style=\"fixed-case\">SV<\/jats:styled-content> sperm cell survival and B double after 24\u00a0hr (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05). However, after 48\u00a0hr, <jats:styled-content style=\"fixed-case\">SV<\/jats:styled-content> sperm cell survival was higher than B double (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). Moreover, multivariate analysis showed that the outcome of single early <jats:styled-content style=\"fixed-case\">TAI<\/jats:styled-content> with <jats:styled-content style=\"fixed-case\">SV<\/jats:styled-content> was not significantly different from B double (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05). Likelihood of pregnancy and calving in the heifer group was higher than in the cow group (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05). These results imply that spermatozoa immobilized in alginate gel have prolonged survival.<\/jats:p>","DOI":"10.1111\/rda.13017","type":"journal-article","created":{"date-parts":[[2017,7,10]],"date-time":"2017-07-10T04:46:44Z","timestamp":1499662004000},"page":"1019-1024","source":"Crossref","is-referenced-by-count":11,"title":["Reproductive performance of immobilized cryopreserved bovine semen used for timed artificial insemination"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4350-1887","authenticated-orcid":false,"given":"AH","family":"Alm\u2010Kristiansen","sequence":"first","affiliation":[{"name":"Department of Natural Sciences and Technology Inland Norway University of Applied Sciences  Hamar Norway"},{"name":"SpermVital AS  Hamar Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0954-3525","authenticated-orcid":false,"given":"G","family":"Dalen","sequence":"additional","affiliation":[{"name":"Tine SA  \u00c5s Norway"},{"name":"Norwegian University of Life Sciences  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Klinkenberg","sequence":"additional","affiliation":[{"name":"SINTEF  Trondheim Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Bekk","sequence":"additional","affiliation":[{"name":"SpermVital AS  Hamar Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LT","family":"Thorkildsen","sequence":"additional","affiliation":[{"name":"SpermVital AS  Hamar Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KE","family":"Waterhouse","sequence":"additional","affiliation":[{"name":"SpermVital AS  Hamar Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Kommisrud","sequence":"additional","affiliation":[{"name":"Department of Natural Sciences and Technology Inland Norway University of Applied Sciences  Hamar Norway"},{"name":"SpermVital AS  Hamar Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.2002.tb02234.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2005.10.005"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(98)75739-X"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1017\/S175173110800236X"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(03)00203-6"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2007-0778"},{"key":"e_1_2_8_8_1","unstructured":"Geno(2015).Annual report 2015. 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