{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T05:02:31Z","timestamp":1774414951981,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,7,9]],"date-time":"2017-07-09T00:00:00Z","timestamp":1499558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The objective of the study was to evaluate the efficacy of ovarian response and pregnancy rate in anovular buffaloes following Ovsynch and Ovsynch Plus protocols. Buffaloes (<jats:italic>n<\/jats:italic>\u00a0=\u00a055) were divided into two groups: Ovsynch group (<jats:italic>n<\/jats:italic>\u00a0=\u00a026): GnRH (10\u00a0\u03bcg, GnRH1) on Day 0, PGF<jats:sub>2<\/jats:sub>\u03b1 (25\u00a0mg) on Day 7, GnRH (10\u00a0\u03bcg, GnRH2) on Day 9; Ovsynch Plus group (<jats:italic>n<\/jats:italic>\u00a0=\u00a029): 500\u00a0IU equine chorionic gonadotropin (eCG) 72\u00a0hr (day \u22123) prior to Ovsynch protocol, followed by fixed timed artificial insemination (FTAI) 6 and 24\u00a0hr after GnRH2 injection in bot groups. Transrectal ultrasonography was performed daily, that is, from day 0 and \u22123 in Ovsynch and Ovsynch Plus group, respectively for ovarian response and pregnancy diagnosis at day 30 post\u2010insemination. In Ovsynch Plus group, administration of eCG prior to GnRH1 increased (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.001) the diameter (mm) of dominant follicle (DF) from 10.15\u00a0\u00b1\u00a00.26 to 12.23\u00a0\u00b1\u00a00.34 within 72\u00a0hr of treatment resulting higher ovulatory response to GnRH1. Ovulation after GnRH1 was higher (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01) in Ovsynch Plus group (96.6%) than Ovsynch group (61.5%). However, ovulation rate to GnRH2 was similar (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.05) between groups (Ovsynch group: 76.9% vs. Ovsynch Plus group: 70.0%). Mean DF diameter (mm) that ovulated to both GnRHs was higher (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.01) than non\u2010ovulated counterparts in both groups (Ovsynch group: 10.80\u00a0\u00b1\u00a00.27 vs. 8.47\u00a0\u00b1\u00a00.53; Ovsynch Plus group: 11.99\u00a0\u00b1\u00a00.24 vs. 9.5\u00a0\u00b1\u00a00.63). Pregnancy was established in buffaloes which responded to both GnRHs, irrespective of groups, being higher (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.52) in Ovsynch Plus group (34.5%) than Ovsynch group (23.1%), though non\u2010significant. In summary, this study showed that eCG inclusion prior to Ovsynch regimen improves ovulatory response in anovular buffaloes during low\u2010breeding season.<\/jats:p>","DOI":"10.1111\/rda.13020","type":"journal-article","created":{"date-parts":[[2017,7,10]],"date-time":"2017-07-10T04:46:42Z","timestamp":1499662002000},"page":"1030-1035","source":"Crossref","is-referenced-by-count":9,"title":["Ovsynch Plus protocol improves ovarian response in anovular Murrah buffaloes in low\u2010breeding season"],"prefix":"10.1111","volume":"52","author":[{"given":"RK","family":"Sharma","sequence":"first","affiliation":[{"name":"ICAR\u2010Central Institute for Research on Buffaloes Hisar Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SK","family":"Phulia","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute for Research on Buffaloes Hisar Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3832-5495","authenticated-orcid":false,"given":"A","family":"Jerome","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute for Research on Buffaloes Hisar Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Singh","sequence":"additional","affiliation":[{"name":"ICAR\u2010Central Institute for Research on Buffaloes Hisar Haryana India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2007.03.011"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livprodsci.2004.06.014"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1413-95962003000600007"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)00639-8"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.10.013"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.09.006"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.11.004"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(98)00211-8"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.09.039"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.07.024"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(03)73921-6"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2009.12.005"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2017.02.022"},{"key":"e_1_2_8_15_1","first-page":"157","article-title":"The effect of pretreatment with progesterone on the oestrous response to oestradiol\u201017\u03b2 benzoate in the postpartum dairy cow","volume":"52","author":"McDougall S.","year":"1992","journal-title":"Proceeding of New Zealand Society of Animal Production"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2012.03.004"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-12-1-27"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.12.012"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2006-592"},{"key":"e_1_2_8_20_1","first-page":"27","article-title":"Enhancing reproductive performance in dairy buffalo, Major constraints and achievements","volume":"61","author":"Nanda A. 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