{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T12:30:32Z","timestamp":1766579432226,"version":"3.37.3"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,7,30]],"date-time":"2017-07-30T00:00:00Z","timestamp":1501372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Leucemia inhibitory factor (<jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content>) is involved in various reproductive processes, including sperm development, regulation of ovulation, as well as blastocyst formation, hatching and implantation in embryos. Moreover,<jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content>has also been shown significantly to enhance the blastocyst formation rates of bovine embryos, a finding that remains controversial. Our purpose was to investigate time\u2010dependent effect of<jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content>on bovine embryo culture, especially in terms of addition timing. Presumptive zygotes were cultured in five different groups. In this study, 100\u00a0ng\/ml<jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content>was added to the culture medium were as follows; control:<jats:styled-content style=\"fixed-case\">SOF<\/jats:styled-content>alone, group A: at day 0 (fertilization day), group B: at day 4 post\u2010insemination (p.i.), group C: at day 4 to 7 (p.i. before vitrification) and group D: at day 8 (p.i. after thawing). Addition of<jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content>to the culture medium at day 4 significantly increased the percentage of blastocyst rate when compared day 0, day 4 at 6\/7 and control group (41.8% versus 24.3%, 19.7%, 34.6%). In conclusion, the addition of<jats:styled-content style=\"fixed-case\">LIF<\/jats:styled-content>only on day 4 (p.i.) to the culture medium was found to be beneficial for bovine embryonic development based on several measures, including blastocysts rate, re\u2010expansion rate and cellular cryotolerance after vitrification.<\/jats:p>","DOI":"10.1111\/rda.13040","type":"journal-article","created":{"date-parts":[[2017,7,31]],"date-time":"2017-07-31T04:12:47Z","timestamp":1501474367000},"page":"1113-1119","source":"Crossref","is-referenced-by-count":7,"title":["Leucemia inhibitory factor; investigating the time\u2010dependent effect on viability of vitrified bovine embryos"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9639-5497","authenticated-orcid":false,"given":"A","family":"Kocyigit","sequence":"first","affiliation":[{"name":"Departments of Reproduction and Artificial Insemination Faculty of Veterinary Medicine Cumhuriyet University Sivas Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Cevik","sequence":"additional","affiliation":[{"name":"Departments of Reproduction and Artificial Insemination Faculty of Veterinary Medicine Ondokuz May\u0131s University Samsun Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,30]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"418","article-title":"What is new in the cryopreservation of embryos?","volume":"12","author":"Alminana C.","year":"2015","journal-title":"Animal Reproduction"},{"issue":"3","key":"e_1_2_7_3_1","first-page":"213","article-title":"Relevance of LIF and EGF on mouse preimplantation embryo development","volume":"10","author":"Amiri I.","year":"2008","journal-title":"Yakhteh Medical Journal"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/12.6.1233"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)61731-2"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod46.3.470"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(94)90383-T"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.2527\/1997.7551332x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1093\/oso\/9780195142266.003.0003","volume-title":"A laboratory guide to the mammalian embryo","author":"Gardner D. 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