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In order to maximize the efficiency of using sex\u2010sorted sperm under in vitro fertilization conditions, the selection of an appropriate sperm separation technique is essential. In this study, the effect of using different silane\u2010coated silica colloid dilutions and layering configurations during centrifugation of sex\u2010sorted sperm was examined over an extended period of incubation time. Sperm recovery and viability after centrifugation using the colloid separation technique were measured along with several sperm motility parameters using CASA. For this purpose, frozen and thawed sex\u2010sorted sperm samples were centrifuged using mini\u2010volume single\u2010layer (40%, 60% and 80%) and mini\u2010volume two\u2010layer (45%\/90%, 40%\/80% and 30%\/60%) separation configurations using PureSperm<jats:sup>\u00ae<\/jats:sup>. A single layer of 40% PureSperm<jats:sup>\u00ae<\/jats:sup>recovered significantly more sex\u2010sorted sperm (78.07%\u00a0\u00b1\u00a02.28%) followed by a single layer of 80% PureSperm<jats:sup>\u00ae<\/jats:sup>(68.43%\u00a0\u00b1\u00a02.33%). The lowest sperm recovery was obtained using a two\u2010layer PureSperm<jats:sup>\u00ae<\/jats:sup>dilution of 45%\/90% (47.57%\u00a0\u00b1\u00a02.33%). Single\u2010layer centrifugation recovered more sorted sperm (68.67%\u00a0\u00b1\u00a01.74%) than two layer (53.74%\u00a0\u00b1\u00a01.74%) (<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>.0001). A single layer of 80% PureSperm<jats:sup>\u00ae<\/jats:sup>exhibited the highest sorted sperm viability (72.01%\u00a0\u00b1\u00a02.90%) after centrifugation (<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>.05). The mini\u2010volume single layer of 80% PureSperm<jats:sup>\u00ae<\/jats:sup>was determined to be an effective alternative to a two\u2010layer centrifugation configuration for sex\u2010sorted sperm selection. In addition, single\u2010layer colloid dilution of 80% performed either as well as or significantly outperformed the other treatments, as well as the control, with regard to motility (MOT) for all time periods of analysis.<\/jats:p>","DOI":"10.1111\/rda.13048","type":"journal-article","created":{"date-parts":[[2017,9,11]],"date-time":"2017-09-11T05:42:39Z","timestamp":1505108559000},"page":"26-33","source":"Crossref","is-referenced-by-count":2,"title":["Effect of different mini\u2010volume colloid centrifugation configurations on flow cytometrically sorted sperm recovery efficiency and quality using a computer\u2010assisted semen analyzer"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2368-7039","authenticated-orcid":false,"given":"LB","family":"Ferr\u00e9","sequence":"first","affiliation":[{"name":"Instituto Nacional de Tecnolog\u00eda Agropecuaria Rafaela, Santa Fe Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JL","family":"Chitwood","sequence":"additional","affiliation":[{"name":"Department of Animal Science University of California Davis CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Fresno","sequence":"additional","affiliation":[{"name":"Computational Genomics Division National Institute of Genomic Medicine (INMEGEN) M\u00e9xico M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HH","family":"Ortega","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Veterinarias Universidad Nacional del Litoral\/CONICET Santa Fe, Esperanza Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ME","family":"Kjelland","sequence":"additional","affiliation":[{"name":"Conservation, Genetics and Biotech, LLC Vicksburg MS USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PJ","family":"Ross","sequence":"additional","affiliation":[{"name":"Department of Animal Science University of California Davis CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,9,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0967199416000150"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2013.09.004"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(94)00037-U"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691X(95)00272-A"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2010.01696.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD09142"},{"key":"e_1_2_9_8_1","first-page":"244","article-title":"Sperm preparation and selection techniques","author":"Beydola T.","year":"2013","journal-title":"Male infertility practice"},{"key":"e_1_2_9_9_1","first-page":"167","volume-title":"Sperm preparation","author":"Bj\u00f6rndahl L.","year":"2010"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.09.017"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod12.2.260"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.06.030"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.03.029"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/13.11.3139"},{"key":"e_1_2_9_15_1","first-page":"205","article-title":"The use of sexed semen on embryo production","volume":"34","author":"Dell'Aqua J. 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