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This study was undertaken to investigate whether uterine fluid biomolecules could act as tool for diagnosis of subclinical endometritis in the buffalo. Uterine fluid samples from normal (<jats:italic>n<\/jats:italic>\u00a0=\u00a022) and subclinical endometritis (<jats:italic>n<\/jats:italic>\u00a0=\u00a018; diagnosed based on uterine cytology)\u2010affected buffaloes were subjected to biochemical analysis. Among the different biochemical constituents estimated, urea, urea N, cholesterol, total bilirubin and alkaline phosphatase (<jats:styled-content style=\"fixed-case\">ALP<\/jats:styled-content>) concentrations were significantly (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) higher in uterine fluid obtained from subclinical endometritis\u2010affected buffaloes. The extent of difference between normal and subclinical endometritis\u2010affected buffaloes was highest in <jats:styled-content style=\"fixed-case\">ALP<\/jats:styled-content> (69%) followed by cholesterol (55%), bilirubin (48%), urea (30%) and urea N (30%) concentrations. Receiver operating characteristic (<jats:styled-content style=\"fixed-case\">ROC<\/jats:styled-content>) analysis indicated that the likelihood ratio (<jats:styled-content style=\"fixed-case\">LR<\/jats:styled-content>) was 3.63 for urea, indicating that buffaloes having less than the threshold concentration (47.5\u00a0mg\/dl) of urea in their uterine fluid were at 3.6 times more risk to be affected with <jats:styled-content style=\"fixed-case\">SE<\/jats:styled-content>. The <jats:styled-content style=\"fixed-case\">LR<\/jats:styled-content>s for urea N, cholesterol, <jats:styled-content style=\"fixed-case\">ALP<\/jats:styled-content> and bilirubin were 2.33, 2.54, 2.12 and 1.65, respectively. It was concluded that <jats:styled-content style=\"fixed-case\">ALP<\/jats:styled-content>, urea, urea N and cholesterol concentrations in uterine fluid may serve as an aid for diagnosing subclinical endometritis in the buffalo.<\/jats:p>","DOI":"10.1111\/rda.13051","type":"journal-article","created":{"date-parts":[[2017,8,14]],"date-time":"2017-08-14T08:12:33Z","timestamp":1502698353000},"page":"48-53","source":"Crossref","is-referenced-by-count":6,"title":["Biochemical analysis of uterine fluid for identification of indicators for subclinical endometritis in the water buffalo (<i>Bubalus bubalis<\/i>)"],"prefix":"10.1111","volume":"53","author":[{"given":"SC","family":"Gahlot","sequence":"first","affiliation":[{"name":"Theriogenology Lab Animal Reproduction Gynaecology &amp; Obstetrics National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Kumar","sequence":"additional","affiliation":[{"name":"Theriogenology Lab Animal Reproduction Gynaecology &amp; Obstetrics National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8061-3477","authenticated-orcid":false,"given":"A","family":"Kumaresan","sequence":"additional","affiliation":[{"name":"Theriogenology Lab Animal Reproduction Gynaecology &amp; Obstetrics National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Vairamuthu","sequence":"additional","affiliation":[{"name":"Centralized Clinical Laboratory Madras Veterinary College TANUVAS  Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KK","family":"Saraf","sequence":"additional","affiliation":[{"name":"Theriogenology Lab Animal Reproduction Gynaecology &amp; Obstetrics National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Sreela","sequence":"additional","affiliation":[{"name":"Theriogenology Lab Animal Reproduction Gynaecology &amp; Obstetrics National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RK","family":"Baithalu","sequence":"additional","affiliation":[{"name":"Livestock Research Centre National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SS","family":"Lathwal","sequence":"additional","affiliation":[{"name":"Livestock Research Centre National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TK","family":"Mohanty","sequence":"additional","affiliation":[{"name":"Artificial Breeding Research Centre National Dairy Research Institute  Karnal Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.03.018"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.01.010"},{"key":"e_1_2_9_4_1","first-page":"154","article-title":"Uterine infection in buffalo: A review","volume":"29","author":"Azawi O. 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