{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T01:32:32Z","timestamp":1773279152008,"version":"3.50.1"},"reference-count":14,"publisher":"Wiley","issue":"S4","license":[{"start":{"date-parts":[[2017,10,20]],"date-time":"2017-10-20T00:00:00Z","timestamp":1508457600000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Rooster fertility peaks between 30 and 40\u00a0weeks of age and declines rapidly from 50\u00a0weeks of age. This is linked to a reduction in the number of spermatozoa in the ejaculate due to high density of spermatids in the seminiferous tubules of low\u2010fertility roosters. In this study, we assessed the effects over spermatogenesis of both letrozole, an aromatase inhibitor, and a commercial herbal extract designed for improving fertility composed by <jats:italic>Tribulus, Cinnamomum, Zingiber<\/jats:italic> and <jats:italic>Sativus<\/jats:italic>. Forty\u2010two\u2010week\u2010old Ross 308 roosters (<jats:italic>n\u00a0<\/jats:italic>=\u00a024) were distributed into four groups: control, letrozole (0.03\u00a0mg\/kg), herbal extract (0.04\u00a0ml\/kg) and letrozole+herbal extract. After 14\u00a0weeks of daily oral supplementation, their testes and epididymides were weighed, fixed and sectioned for assessment of spermatogenesis and quantification of sperm cells inside the lumen. Differences in seminiferous tubules measurements and density of sperm cells were tested using R software (version 3.0.1). Although body weight was not affected by the treatment, testes from animals treated with the combination of letrozole and herbal extracts were heavier than those from control animals. Animals treated with either letrozole or herbal extract, or their combination, showed a significant higher number of sperm cells inside the seminiferous tubules and epididymis than control animals (<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>.05). These data suggest that the use of letrozole and the herbal extract could improve sperm cell production in ageing roosters. Future studies are needed to disclose the causal mechanisms involved and its effect on fertility and ejaculate features.<\/jats:p>","DOI":"10.1111\/rda.13058","type":"journal-article","created":{"date-parts":[[2017,10,20]],"date-time":"2017-10-20T05:25:16Z","timestamp":1508477116000},"page":"58-60","source":"Crossref","is-referenced-by-count":16,"title":["Increased sperm cell production in ageing roosters by an oral treatment with an aromatase inhibitor and a natural herbal extract designed for improving fertility"],"prefix":"10.1111","volume":"52","author":[{"given":"H","family":"Adeldust","sequence":"first","affiliation":[{"name":"Department of Animal Science College of Agriculture University of Kurdistan  Sanandaj Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Farzinpour","sequence":"additional","affiliation":[{"name":"Department of Animal Science College of Agriculture University of Kurdistan  Sanandaj Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Farshad","sequence":"additional","affiliation":[{"name":"Department of Animal Science College of Agriculture University of Kurdistan  Sanandaj Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Rostamzadeh","sequence":"additional","affiliation":[{"name":"Department of Animal Science College of Agriculture University of Kurdistan  Sanandaj Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Lopez\u2010Bejar","sequence":"additional","affiliation":[{"name":"Veterinary Faculty Universitat Aut\u00f2noma de Barcelona  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/tam.4.3.163.169"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2013-03617"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0680558"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/aja.2011.44"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jps.1124"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101-005-2833-x"},{"key":"e_1_2_7_8_1","first-page":"1","article-title":"Morphometric evaluation of seminiferous tubules in aged mice testes after melatonin administration","volume":"13","author":"Mehraein F.","year":"2011","journal-title":"Cell Journal (Yakhteh)"},{"key":"e_1_2_7_9_1","volume-title":"Nutrient Requirements of Poultry: Ninth Revised Edition, 1994","author":"National Research Council","year":"1994"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1071\/AN12257"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/153537020222700917"},{"key":"e_1_2_7_12_1","first-page":"829","article-title":"Use of the aromatase inhibitor letrozole to treat male infertility","volume":"92","author":"Patry G.","year":"2010","journal-title":"Fertility and Sterility (case report)"},{"key":"e_1_2_7_13_1","unstructured":"R Core Team(2014).R: A language and environment for statistical computing.R Foundation for Statistical Computing Vienna Austria. 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