{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T07:01:05Z","timestamp":1768546865082,"version":"3.49.0"},"reference-count":16,"publisher":"Wiley","issue":"S4","license":[{"start":{"date-parts":[[2017,10,20]],"date-time":"2017-10-20T00:00:00Z","timestamp":1508457600000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["AGL2013\u201047798\u2010P"],"award-info":[{"award-number":["AGL2013\u201047798\u2010P"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The presence of bacteria adversely affects boar sperm quality of seminal doses intended for artificial insemination. Currently, the most common measure to prevent bacteriospermia is the addition of antibiotics in semen extenders; however, mounting evidence shows that microbial resistance exists. A promising alternative to replace antibiotics are antimicrobial peptides. In this study, the effects of the antimicrobial peptide protegrine 1 (<jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content>1) on the sperm viability and bacterial load of boar seminal doses were evaluated. Three different concentrations of <jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content>1 (2.5, 25 and 100\u00a0\u03bcg\/ml) were tested over a storing period of 10\u00a0days at 17\u00b0C. Sperm viability was analysed by fluorescence microscopy (<jats:styled-content style=\"fixed-case\">SYBR<\/jats:styled-content>14\/propidium iodide), and bacterial load was assessed by plating 100\u00a0\u03bcl of each sample in Luria\u2013Bertani medium and incubated at 37\u00b0C for 72\u00a0hr under aerobic conditions. Protegrine 1 was effective in controlling the bacterial load in all the assessed concentrations (<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>.05), reaching the lowest values at the highest concentrations of the antimicrobial peptide. Nevertheless, sperm viability was significantly (<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>.05) reduced by all tested concentrations of this peptide, the most cytotoxic effects being observed at the highest <jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content>1 concentrations. Despite these results, the use of <jats:styled-content style=\"fixed-case\">PG<\/jats:styled-content>1 as an alternative to antibiotics cannot be totally discarded, as further studies using the truncated form of this peptide are needed.<\/jats:p>","DOI":"10.1111\/rda.13061","type":"journal-article","created":{"date-parts":[[2017,10,20]],"date-time":"2017-10-20T05:25:16Z","timestamp":1508477116000},"page":"69-71","source":"Crossref","is-referenced-by-count":17,"title":["Effects of the antimicrobial peptide protegrine 1 on sperm viability and bacterial load of boar seminal doses"],"prefix":"10.1111","volume":"52","author":[{"given":"S","family":"Sancho","sequence":"first","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm) Department of Biology INTEA University of Girona Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Briz","sequence":"additional","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm) Department of Biology INTEA University of Girona Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Yeste","sequence":"additional","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm) Department of Biology INTEA University of Girona Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Bonet","sequence":"additional","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm) Department of Biology INTEA University of Girona Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Bussalleu","sequence":"additional","affiliation":[{"name":"Biotechnology of Animal and Human Reproduction (TechnoSperm) Department of Biology INTEA University of Girona Catalonia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(00)00261-2"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-35049-8_10"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"2486","DOI":"10.1128\/IAI.66.6.2486-2493.1998","article-title":"Activity of protegrins against yeast\u2010phase Candida albicans","volume":"66","author":"Cho Y.","year":"1998","journal-title":"Infection and Immunity"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod59.1.7"},{"issue":"1","key":"e_1_2_7_6_1","first-page":"1","article-title":"Antimicrobial peptides: An overview of a promising class of therapeutics","volume":"2","author":"Giuliani A.","year":"2007","journal-title":"Central European Journal of Biology"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568005024605855"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.09.049"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2011.11.002"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.06.018"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001067"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.07.016"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100490"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.43.853"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(01)75802-X"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/415389a"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics3040694"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Frda.13061","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.13061","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T02:20:58Z","timestamp":1696040458000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.13061"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10]]},"references-count":16,"journal-issue":{"issue":"S4","published-print":{"date-parts":[[2017,10]]}},"alternative-id":["10.1111\/rda.13061"],"URL":"https:\/\/doi.org\/10.1111\/rda.13061","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10]]}}}