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However, the modifications in spermatic features and the oxidative profile are not fully understood in cats. This information is necessary for the development of biotechniques, such as new extenders for cryopreservation. Therefore, the purpose of this study was to evaluate the spermatic and oxidative profile in samples from the epididymal cauda of domestic cats cooled at 5\u00b0C for 24, 48 and 72\u00a0hr. Spermatozoa were collected from the epididymis cauda. Evaluations consisted of computer\u2010assisted sperm analysis (<jats:styled-content style=\"fixed-case\">CASA<\/jats:styled-content>), plasma membrane integrity (eosin\/nigrosin), acrosome integrity (fast green\/rose bengal), sperm morphology, sperm <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> integrity (toluidine blue), mitochondrial activity (3\u20323 diaminobenzidine), activity of the antioxidant enzymes glutathione peroxidase (<jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content>x) and superoxide dismutase (<jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content>), measurement of lipid peroxidation (<jats:styled-content style=\"fixed-case\">TBARS<\/jats:styled-content>) and protein oxidation. A decrease in sperm motility parameters was observed after 72\u00a0hr of cooling (i.e. total and progressive) with a higher percentage of minor (37.7\u00a0\u00b1\u00a06.3%) and total defects (53.4\u00a0\u00b1\u00a06.3%). Additionally, a decrease in high mitochondrial activity (Class I: 16.6\u00a0\u00b1\u00a02.2%) occurred after 72\u00a0hr. The decrease in motility rates after a long cooling time probably was caused by the increase in sperm abnormalities. A long cooling time causes cold shock and mitochondrial exhaustion, but there was no observed change with the oxidative stress condition. Therefore, cat epididymal sperm stored at 5\u00b0C appear to maintain a high quality for up to 48\u00a0hr of cooling time.<\/jats:p>","DOI":"10.1111\/rda.13086","type":"journal-article","created":{"date-parts":[[2017,10,31]],"date-time":"2017-10-31T03:42:39Z","timestamp":1509421359000},"page":"163-170","source":"Crossref","is-referenced-by-count":6,"title":["Spermatic and oxidative profile of domestic cat (<i>Felis catus<\/i>) epididymal sperm subjected to different cooling times (24, 48 and 72\u00a0hours)"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4989-364X","authenticated-orcid":false,"given":"DSR","family":"Angrimani","sequence":"first","affiliation":[{"name":"Department of Animal Reproduction School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KK","family":"Nagai","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"BR","family":"Rui","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LC","family":"Bicudo","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JDA","family":"Losano","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MM","family":"Brito","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MCP","family":"Francischini","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4262-0825","authenticated-orcid":false,"given":"M","family":"Nichi","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gap059"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-13-0178"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.10.015"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2017.03.014"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2014.10.023"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.03.022"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2007.00780.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)00170-3"},{"key":"e_1_2_7_10_1","volume-title":"Abnormal morphology of bovine spermatozoa","author":"Barth A. 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