{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:09:01Z","timestamp":1791515341589,"version":"4.3.4"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,10,28]],"date-time":"2017-10-28T00:00:00Z","timestamp":1509148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2018,2]]},"abstract":"Contents<\/jats:title>The objective of this study was to compare different extenders for post\u2010thaw in vitro sperm function and in vivo fertility of buffalo semen. Accordingly, sperm of 30 ejaculates extended in egg yolk (TRIS<\/jats:styled-content> with 20% egg yolk; EY<\/jats:styled-content>), two soya lecithin\u2010based (SL<\/jats:styled-content>\u20101; AndroMed\u00ae<\/jats:sup> and SL<\/jats:styled-content>\u20102; Bioxcell\u00ae<\/jats:sup>) and a liposome\u2010based extender (LS<\/jats:styled-content>; OptiXcell\u00ae<\/jats:sup>) were tested. The post\u2010thaw semen was evaluated for computer\u2010assisted sperm analysis (CASA<\/jats:styled-content>), sperm viability, membrane and acrosome integrity, DNA<\/jats:styled-content> integrity and acrosome reaction and first service pregnancy rate (FSPR<\/jats:styled-content>) in a fixed\u2010time artificial insemination programme. Total motility and VCL<\/jats:styled-content> were the only CASA<\/jats:styled-content>\u2010based parameters that exhibited significantly higher (p<\/jats:italic>\u00a0<\u00a0.05) percentage in LS<\/jats:styled-content> among these extenders. Post\u2010thaw percentage of acrosome integrity (55.9\u00a0\u00b1\u00a01.4, 58.1\u00a0\u00b1\u00a02.0, 55.8\u00a0\u00b1\u00a02.0, 56.6\u00a0\u00b1\u00a02.3) and DNA<\/jats:styled-content> integrity (68.8\u00a0\u00b1\u00a02.0, 69.2\u00a0\u00b1\u00a02.3, 71.3\u00a0\u00b1\u00a02.1, 69.1\u00a0\u00b1\u00a02.1) did not differ (p<\/jats:italic>\u00a0>\u00a0.05) in EY<\/jats:styled-content>, SL<\/jats:styled-content>\u20101, SL<\/jats:styled-content>\u20102 and LS<\/jats:styled-content> extender, respectively. However, a variable response in terms of efficacy of different extenders for sperm viability and plasma membrane integrity was observed. Assessment of inducibility of acrosome reaction showed significant differences between extenders (51.9\u00a0\u00b1\u00a02.1, 44.3\u00a0\u00b1\u00a02.4, 46.1\u00a0\u00b1\u00a02.3 and 58.1\u00a0\u00b1\u00a03.1%, respectively, for EY<\/jats:styled-content>, SL<\/jats:styled-content>\u20101, SL<\/jats:styled-content>\u20102 and LS<\/jats:styled-content>). Furthermore, field trials revealed significantly higher (p<\/jats:italic>\u00a0<\u00a0.05) FSPR<\/jats:styled-content> of LS<\/jats:styled-content>\u2010extended semen as compared to that for EY<\/jats:styled-content>, SL<\/jats:styled-content>\u20101 and SL<\/jats:styled-content>\u20102 extender (46.3%, 41.2%, 31.2% and 29.7%, respectively). It is concluded that the liposome\u2010based extender is more effective than egg yolk\u2010 and soya lecithin\u2010based extenders and may be used for cryopreservation of buffalo semen in the future.<\/jats:p>","DOI":"10.1111\/rda.13092","type":"journal-article","created":{"date-parts":[[2017,10,28]],"date-time":"2017-10-28T05:46:34Z","timestamp":1509169594000},"page":"195-202","source":"Crossref","is-referenced-by-count":30,"title":["Comparison of in vitro and in vivo fertilizing potential of buffalo bull semen frozen in egg yolk\u2010, soya bean lecithin\u2010 and liposome\u2010based extenders"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2164-764X","authenticated-orcid":false,"given":"AK","family":"Singh","sequence":"first","affiliation":[{"name":"Guru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Kumar","sequence":"additional","affiliation":[{"name":"Guru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Honparkhe","sequence":"additional","affiliation":[{"name":"Guru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Kaur","sequence":"additional","affiliation":[{"name":"Guru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Kaur","sequence":"additional","affiliation":[{"name":"Guru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SPS","family":"Ghuman","sequence":"additional","affiliation":[{"name":"Guru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PS","family":"Brar","sequence":"additional","affiliation":[{"name":"Guru Angad Dev Veterinary and Animal Sciences University Ludhiana Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)01369-9"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.04.024"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00011"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(03)00259-0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01240.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.06.095"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.20565"},{"key":"e_1_2_9_9_1","first-page":"648","article-title":"The evaluation of bull semen with special reference to its use in artificial insemination","volume":"19","author":"Blom E.","year":"1977","journal-title":"Animal Breeding Abstracts"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2007.02.001"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2012.05.011"},{"key":"e_1_2_9_12_1","first-page":"229","article-title":"Comparative efficiency of two freezing extenders for bovine semen based on sperm motility and membrane integrity","volume":"22","author":"Crespilho A. 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