{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T05:17:41Z","timestamp":1777526261329,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,12,12]],"date-time":"2017-12-12T00:00:00Z","timestamp":1513036800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"University of Goettingen"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>The flagellar beating of a spermatozoa's axoneme is caused by the varying activation and inactivation of dynein molecules. Dynein, axonemal, light chain 4 (<jats:italic><jats:styled-content style=\"fixed-case\">DNAL<\/jats:styled-content>4<\/jats:italic>) is a functional candidate gene for sperm motility as it encodes a small subunit of the dyneins. We resequenced the porcine <jats:italic><jats:styled-content style=\"fixed-case\">DNAL<\/jats:styled-content>4<\/jats:italic> using three artificial insemination (<jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>) boars each with high (&gt;68%) or low (&lt;60%) motility, and detected 23 <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content>. These were then genotyped for 82 <jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content> boars. Using spermatological records, significantly negative genetic correlations between ejaculate volume (<jats:styled-content style=\"fixed-case\">VOL<\/jats:styled-content>) and the further spermatological parameters concentration (<jats:styled-content style=\"fixed-case\">CONC<\/jats:styled-content>) (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u2212.43), motility of undiluted semen (<jats:styled-content style=\"fixed-case\">MOTUD<\/jats:styled-content>) (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u2212.09), motility after 24\u00a0h (<jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>1) (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u2212.17) and after 48\u00a0hr (<jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>2) (<jats:italic>r<\/jats:italic>\u00a0=\u00a0\u2212.23) were estimated. Significantly positive correlations existed between <jats:styled-content style=\"fixed-case\">CONC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>1 (<jats:italic>r<\/jats:italic>\u00a0=\u00a0.07) as well as <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>2 (<jats:italic>r<\/jats:italic>\u00a0=\u00a0.10), between <jats:styled-content style=\"fixed-case\">MOTUD<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>1 (<jats:italic>r<\/jats:italic>\u00a0=\u00a0.33), between <jats:styled-content style=\"fixed-case\">MOTUD<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>2 (<jats:italic>r<\/jats:italic>\u00a0=\u00a0.36), and finally between <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>2 (<jats:italic>r<\/jats:italic>\u00a0=\u00a0.70). Significantly negatively correlated were all motility traits with the parameters abnormal acrosome (<jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>) (<jats:styled-content style=\"fixed-case\">MOTUD <\/jats:styled-content><jats:italic>r<\/jats:italic>\u00a0=\u00a0\u2212.06; <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>1 <jats:italic>r<\/jats:italic>\u00a0=\u00a0\u2212.08, and <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>2 <jats:italic>r<\/jats:italic>\u00a0=\u00a0\u2212.1) and presence of cytoplasmic droplet (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>) (<jats:styled-content style=\"fixed-case\">MOTUD <\/jats:styled-content><jats:italic>r<\/jats:italic>\u00a0=\u00a0\u2212.07; <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>1 <jats:italic>r<\/jats:italic>\u00a0=\u00a0\u2212.08; <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>2 <jats:italic>r<\/jats:italic>\u00a0=\u00a0\u2212.07). Association analyses (single marker regression model; <jats:styled-content style=\"fixed-case\">SMR<\/jats:styled-content>) propose that <jats:styled-content style=\"fixed-case\">SNP<\/jats:styled-content> g.1007A&gt;G, located in the second intron, reduces motility significantly (<jats:styled-content style=\"fixed-case\">MOTUD<\/jats:styled-content> \u20104.59%; <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>1 \u201010.33%; <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>2 \u201019.37%). According to the dominant\u2010recessive model (<jats:styled-content style=\"fixed-case\">DRM<\/jats:styled-content>), genotype <jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content> is always superior compared to genotypes <jats:styled-content style=\"fixed-case\">AG<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GG<\/jats:styled-content> (i.e. <jats:styled-content style=\"fixed-case\">MOTUD<\/jats:styled-content> 67.67%, 64.16% and 53.91%; <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>1 54.17%, 43.75% and 28.44%; <jats:styled-content style=\"fixed-case\">MOT<\/jats:styled-content>2 44.12%, 24.91% and 4.97%). The average effect of gene substitution (g.1007A&gt;G) on abnormal midpiece (<jats:styled-content style=\"fixed-case\">AM<\/jats:styled-content>) was 0.71%, the genotypic values\u2014as expressed by <jats:styled-content style=\"fixed-case\">LS<\/jats:styled-content>means\u2014were 0.1 (<jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content>) and 0.81 (<jats:styled-content style=\"fixed-case\">AG<\/jats:styled-content>).<\/jats:p>","DOI":"10.1111\/rda.13120","type":"journal-article","created":{"date-parts":[[2017,12,13]],"date-time":"2017-12-13T04:48:58Z","timestamp":1513140538000},"page":"401-413","source":"Crossref","is-referenced-by-count":4,"title":["<scp>SNP<\/scp> g.1007A&gt;G within the porcine <scp>DNAL<\/scp>4 gene affects sperm motility traits and percentage of midpiece abnormalities"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8062-6462","authenticated-orcid":false,"given":"I","family":"Wiedemann","sequence":"first","affiliation":[{"name":"Department of Animal Sciences, Livestock Biotechnology and Reproduction University of Goettingen  Goettingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Maehlmeyer","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Livestock Biotechnology and Reproduction University of Goettingen  Goettingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Jansen","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Livestock Biotechnology and Reproduction University of Goettingen  Goettingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"AR","family":"Sharifi","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Animal Breeding and Genetics University of Goettingen  Goettingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Knorr","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, Livestock Biotechnology and Reproduction University of Goettingen  Goettingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,12]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0272.2011.01229.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0952836905007284"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-4320(90)90039-I"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-35049-8"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1995.tb01751.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddh273"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007186405678"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2004.01.015"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-007-0460-x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1751-0147-50-48"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2011.01968.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.08.008"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.humrep.a137283"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.106.058669"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12533"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.02.017"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1135308"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2005.05.023"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2011.03.007"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2014.09.021"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.3.1215"},{"key":"e_1_2_8_23_1","volume-title":"K\u00fcnstliche Besamung und Eitransplantation bei Tier und Mensch","author":"Paufler S. 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