{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T14:19:44Z","timestamp":1788963584764,"version":"build-2803163510"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,12,11]],"date-time":"2017-12-11T00:00:00Z","timestamp":1512950400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Increasing use of fixed\u2010time artificial insemination (<jats:styled-content style=\"fixed-case\">FTAI<\/jats:styled-content>) in beef cattle production has presented an opportunity for the use of fresh or chilled semen as an alternative to standard cryopreserved semen. The objective of this study was to examine in vitro sperm function and pregnancy rate of electroejaculated semen, chilled and stored for 48\u00a0hr, compared to conventionally cryopreserved semen with an optimized<jats:styled-content style=\"fixed-case\">FTAI<\/jats:styled-content>protocol in Brahman cattle. Semen from three Brahman bulls was collected, and aliquots were extended in either chilled (at 5\u00b0C) or frozen (<jats:styled-content style=\"fixed-case\">LN<\/jats:styled-content><jats:sub>2<\/jats:sub>) in a Tris\u2010egg yolk extender base with 2.4% or 7.0% glycerol, respectively. Semen samples were assessed 48\u00a0hr after collection or post\u2010thaw and warming, for sperm motility, in vitro sperm function and fertilizing ability, and used in a<jats:styled-content style=\"fixed-case\">FTAI<\/jats:styled-content>programme. The overall pregnancy rates was significantly different (<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>.01) after<jats:styled-content style=\"fixed-case\">FTAI<\/jats:styled-content>with frozen (<jats:italic>n<\/jats:italic>\u00a0=\u00a0173; 53.2%) and chilled semen (<jats:italic>n<\/jats:italic>\u00a0=\u00a0174; 31.6%). In contrast, the in vitro sperm assessment showed that the chilled semen had significantly faster motility (<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>.05), a higher proportion of progressively motile spermatozoa (<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>.05), with significantly higher proportions of acrosome intact, viable spermatozoa (<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>.01). This study showed that reasonable pregnancy rates in Brahman cattle can be achieved using<jats:styled-content style=\"fixed-case\">FTAI<\/jats:styled-content>with chilled semen collected using electroejaculation and stored for up to 48\u00a0hr. However, improvements in semen extenders are required in consideration of semen collection method to improve the longevity of sperm fertilizing ability to significantly increase<jats:styled-content style=\"fixed-case\">FTAI<\/jats:styled-content>output using chilled storage of bull semen.<\/jats:p>","DOI":"10.1111\/rda.13121","type":"journal-article","created":{"date-parts":[[2017,12,11]],"date-time":"2017-12-11T09:28:46Z","timestamp":1512984526000},"page":"414-422","source":"Crossref","is-referenced-by-count":4,"title":["Investigation of in vitro measurable sperm attributes and their influence on electroejaculated bull semen with a fixed\u2010time artificial insemination protocol in Australian<i>Bos indicus<\/i>cattle"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4895-7437","authenticated-orcid":false,"given":"N","family":"Satake","sequence":"first","affiliation":[{"name":"School of Veterinary Science The University of Queensland Gatton QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SAA","family":"Edwards","sequence":"additional","affiliation":[{"name":"School of Veterinary Science The University of Queensland Gatton QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Tutt","sequence":"additional","affiliation":[{"name":"School of Veterinary Science The University of Queensland Gatton QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MR","family":"McGowan","sequence":"additional","affiliation":[{"name":"School of Veterinary Science The University of Queensland Gatton QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"GB","family":"Boe\u2010Hansen","sequence":"additional","affiliation":[{"name":"School of Veterinary Science The University of Queensland Gatton QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.0.1220265"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9950659"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2008.04.015"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD14185"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.12.009"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2011.01748.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.11.001"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2012.02028.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1052"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2014.02.020"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3844\/ajavsp.2012.175.179"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2012.05.011"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.S0022-0302(63)89041-4"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-375-6_52"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.08.017"},{"key":"e_1_2_8_17_1","volume-title":"Evaluating and reporting bull fertility","author":"Entwistle K.","year":"2003"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009844109023"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4320(02)00018-0"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.03.009"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2012.02.007"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas.2005-766"},{"key":"e_1_2_8_23_1","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1002\/j.1939-4640.1990.tb01583.x","article-title":"Cryopreservation of mammalian sperm: what we ask them to survive","volume":"11","author":"Hammerstedt R. 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