{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T02:40:47Z","timestamp":1790304047938,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,12,14]],"date-time":"2017-12-14T00:00:00Z","timestamp":1513209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["105\u20102313\u2010B\u2010002\u2010039\u2010MY3"],"award-info":[{"award-number":["105\u20102313\u2010B\u2010002\u2010039\u2010MY3"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001868","name":"National Science Council","doi-asserted-by":"publisher","award":["105\u20102313\u2010B\u2010020\u2010001\u2010MY2"],"award-info":[{"award-number":["105\u20102313\u2010B\u2010020\u2010001\u2010MY2"]}],"id":[{"id":"10.13039\/501100001868","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007841","name":"Council of Agriculture","doi-asserted-by":"publisher","award":["106AS\u20102.1.4\u2010AD\u2010U1"],"award-info":[{"award-number":["106AS\u20102.1.4\u2010AD\u2010U1"]}],"id":[{"id":"10.13039\/100007841","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Contents<\/jats:title><jats:p>Amniotic fluid is a rich source of multipotent mesenchymal stem cells (<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s). Amniotic fluid stem cells (<jats:styled-content style=\"fixed-case\">AFSC<\/jats:styled-content>s) have become a new source of stem cells; they have low immunogenicity and are easily harvested. For this reason, they may be useful in clinical tissue engineering. Moreover, <jats:styled-content style=\"fixed-case\">AFSC<\/jats:styled-content>s have anti\u2010inflammatory properties and can repair tissues. This study evaluated the utility of <jats:styled-content style=\"fixed-case\">AFSC<\/jats:styled-content> injection to treat bilateral ovarian dystrophy in Holstein\u2010Friesian cows. Bovine <jats:styled-content style=\"fixed-case\">AFSC<\/jats:styled-content>s (<jats:styled-content style=\"fixed-case\">BAFSC<\/jats:styled-content>s) were collected at slaughter from Holstein\u2010Friesian cows during the third or fourth month of pregnancy and cultured in vitro. The <jats:styled-content style=\"fixed-case\">BAFSC<\/jats:styled-content>s began to show a fibroblast\u2010like morphology. They were positive for \u03b2\u2010integrin, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>44, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>73, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>106 and Oct4 and negative for <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34 and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45. After induction, the cells differentiated into mesodermal lineages. Bilateral ovarian dystrophy was confirmed by ultrasonography in 16 lactating cows. The subsequent experiment lasted 15\u00a0weeks. Serum was collected weekly to analyse progesterone concentrations, and weekly ultrasonography recorded ovarian changes. Each cow was equipped with an automatic heat detection system to facilitate oestrus observation and breeding records. The progesterone concentration of two cows in the treatment group (25%) significantly increased during weeks 10\u201315. On ultrasonography, the treatment group demonstrated mature follicles after <jats:styled-content style=\"fixed-case\">BAFSC<\/jats:styled-content>s injection, and foetuses were visualized approximately 40\u00a0days after artificial insemination (<jats:styled-content style=\"fixed-case\">AI<\/jats:styled-content>). Oestrus rates in the control and treatment groups were 0% (0\/8) and 50% (4\/8), respectively; pregnancy rates were 0% (0\/8) and 25% (2\/8), respectively. Calves were successfully delivered in both cases of pregnancy. These results show that <jats:styled-content style=\"fixed-case\">BAFSC<\/jats:styled-content>s can alleviate bovine ovarian dystrophy and restore fertility.<\/jats:p>","DOI":"10.1111\/rda.13123","type":"journal-article","created":{"date-parts":[[2017,12,14]],"date-time":"2017-12-14T06:12:43Z","timestamp":1513231963000},"page":"433-441","source":"Crossref","is-referenced-by-count":16,"title":["Therapeutic potential of amniotic fluid stem cells to treat bilateral ovarian dystrophy in dairy cows in a subtropical region"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9882-837X","authenticated-orcid":false,"given":"L\u2010B","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Animal Science and Technology National Taiwan University  Taipei Taiwan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u2010Y","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Animal Science National Pingtung University of Science and Technology  Pingtung Taiwan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u2010J","family":"Chou","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology National Taiwan University  Taipei Taiwan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y\u2010J","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neurology Chang Gung Memorial Hospital  Taoyuan Taiwan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u2010S","family":"Shiu","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology National Taiwan University  Taipei Taiwan China"},{"name":"Agriculture Experiment Station College of Bioresources and Agriculture National Taiwan University  Taipei Taiwan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u2010A","family":"Tu","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology National Taiwan University  Taipei Taiwan China"},{"name":"Hsinchu Branch Institute Council of Agriculture Taiwan Livestock Research Institute  Taiwan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u2010X","family":"Gao","sequence":"additional","affiliation":[{"name":"Agriculture Experiment Station College of Bioresources and Agriculture National Taiwan University  Taipei Taiwan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y\u2010C","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology National Taiwan University  Taipei Taiwan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u2010K","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology National Taiwan University  Taipei Taiwan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u2010C","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology National Taiwan University  Taipei Taiwan China"},{"name":"Institute of Biotechnology National Taiwan University  Taipei Taiwan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.2003.1268"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-010-9200-z"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-6226(03)00112-X"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0076118"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2008.01210.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1274"},{"key":"e_1_2_8_8_1","unstructured":"I. 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