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Previous studies have shown that mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s regulate critical genes related to lung development, cell differentiation, surfactant synthesis, secretion and lung disease. This study aimed to examine differentially expressed mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in collapsed lungs of cloned bovine neonates and normal lungs in order to identify key pathways and functions that might be related to the pathogenesis of neonatal respiratory distress. In this study, mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> transcriptomes of collapsed lungs of neonatal cloned bovines and normal lungs were analysed by next\u2010generation sequencing and the results were validated using quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>). A total of 177 differentially expressed mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were identified in the two groups (fold change\u00a0&gt;\u00a02, <jats:styled-content style=\"fixed-case\">RPM<\/jats:styled-content>\u00a0\u2265\u00a05), some of which were associated with type <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> cell differentiation, for example, mmu\u2010miR\u201029a\u20105p_L\u20102R+1, hsa\u2010miR\u2010200c\u20105p_L\u20101R+1 and mmu\u2010miR\u201018a\u20103p_R+1. The differentially expressed mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were predicted to 6,031 target genes. By Gene Ontology (<jats:styled-content style=\"fixed-case\">GO<\/jats:styled-content>) and Kyoto Encyclopeida of Genes and Genomes (<jats:styled-content style=\"fixed-case\">KEGG<\/jats:styled-content>) <jats:styled-content style=\"fixed-case\">DATA<\/jats:styled-content> base, 133 significant <jats:styled-content style=\"fixed-case\">GO<\/jats:styled-content> terms (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) and 13 significant <jats:styled-content style=\"fixed-case\">KEGG<\/jats:styled-content> pathways (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.05) were obtained. Many of them were associated with lung development and surfactant homoeostasis, such as lipid biosynthetic processes, protein transport, endocytosis, lysosome, endosome, Golgi apparatus and membrane. Our results of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s express profiles may partially explain the respiratory distress and lung collapse in neonatal bovine clones and could provide novel insights into roles of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in regulation of lung collapse and neonatal respiratory distress in cloned farm animals.<\/jats:p>","DOI":"10.1111\/rda.13144","type":"journal-article","created":{"date-parts":[[2018,2,8]],"date-time":"2018-02-08T06:32:46Z","timestamp":1518071566000},"page":"550-555","source":"Crossref","is-referenced-by-count":4,"title":["Profiling of mi<scp>RNA<\/scp>s in neonatal cloned bovines with collapsed lungs and respiratory distress"],"prefix":"10.1111","volume":"53","author":[{"given":"FY","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Animal Sciences Chinese Academy of Agricultural Sciences  Beijing China"},{"name":"College of Biological Sciences China 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