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Fifty\u2010one bitches divided into two groups were used. Group 1 (G1) included 28 bitches that had delivered healthy litters and group 2 (G2) included 23 bitches that had delivered puppies who died in the neonatal period. Two vaginal samples were taken, one in proestrus and the other at the end of gestation (<jats:styled-content style=\"fixed-case\">EG<\/jats:styled-content>). Beta\u2010haemolytic <jats:italic>Streptococcus<\/jats:italic> (<jats:styled-content style=\"fixed-case\">BS<\/jats:styled-content>) was isolated from 16 bitches (57%) in G1 and from 21 bitches (91%) in G2. The bacteriological cultures, serological tests (Streptex<jats:sup>\u00ae<\/jats:sup>) and <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assay allowed identification of <jats:italic>Streptococcus canis<\/jats:italic> and <jats:italic>Streptococcus dysgalactiae<\/jats:italic> in G1 and G2. Ultramicroscopic studies allowed the observation of M Protein and capsules in strains of <jats:italic>S.\u00a0dysgalactiae<\/jats:italic> and <jats:italic>S.\u00a0canis<\/jats:italic> in G1 and G2. The <jats:italic>S.\u00a0canis<\/jats:italic> strains isolated from G2 showed thicker capsules than <jats:italic>S.\u00a0canis<\/jats:italic> strains isolated from G1 (234\u00a0\u00b1\u00a024.2 vs 151.23\u00a0\u00b1\u00a028.93\u00a0nm; <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a0.001.). No differences were observed in capsule thickness between strains of <jats:italic>S.\u00a0dysgalactiae<\/jats:italic> isolated from G1 and G2 (210\u00a0\u00b1\u00a013.54 vs 211.66\u00a0\u00b1\u00a019.67\u00a0nm; <jats:italic>p<\/jats:italic>\u00a0&gt;\u00a0.70). All strains of beta\u2010haemolytic <jats:italic>Streptococcus<\/jats:italic> isolated were penicillin sensitive. Penicillin was administered from <jats:styled-content style=\"fixed-case\">EG<\/jats:styled-content> to 5\u00a0days <jats:italic>post\u2010partum<\/jats:italic> in 10\u00a0G2 females with isolation of <jats:styled-content style=\"fixed-case\">BS<\/jats:styled-content> (G2A). Saline solution was administered in eleven G2 females with isolation of <jats:styled-content style=\"fixed-case\">BS<\/jats:styled-content> (G2B). Ninety per cent of the puppies survived in G2A and 25% survived in G2B. Our results suggest <jats:styled-content style=\"fixed-case\">BS<\/jats:styled-content> is involved in canine neonatal deaths.<\/jats:p>","DOI":"10.1111\/rda.13147","type":"journal-article","created":{"date-parts":[[2018,2,19]],"date-time":"2018-02-19T04:27:07Z","timestamp":1519014427000},"page":"609-616","source":"Crossref","is-referenced-by-count":11,"title":["Vaginal isolation of beta\u2010haemolytic <i>Streptococcus<\/i> from bitches with and without neonatal deaths in the litters"],"prefix":"10.1111","volume":"53","author":[{"given":"AE","family":"Guerrero","sequence":"first","affiliation":[{"name":"C\u00e1tedra y Servicio de Reproducci\u00f3n Animal  La Plata Argentina"},{"name":"C\u00e1tedra de Microbiolog\u00eda  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MC","family":"Stornelli","sequence":"additional","affiliation":[{"name":"C\u00e1tedra y Servicio de Reproducci\u00f3n Animal  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SB","family":"Jurado","sequence":"additional","affiliation":[{"name":"Servicio Central de Microscop\u00eda Electr\u00f3nica  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G","family":"Giacoboni","sequence":"additional","affiliation":[{"name":"C\u00e1tedra de Microbiolog\u00eda  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"GH","family":"Sguazza","sequence":"additional","affiliation":[{"name":"Laboratorio de Virolog\u00eda Facultad de Ciencias Veterinarias Universidad Nacional de La Plata  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RL","family":"de la Sota","sequence":"additional","affiliation":[{"name":"C\u00e1tedra y Servicio de Reproducci\u00f3n Animal  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7285-1814","authenticated-orcid":false,"given":"MA","family":"Stornelli","sequence":"additional","affiliation":[{"name":"C\u00e1tedra y Servicio de Reproducci\u00f3n Animal  La Plata Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.2015.1016133"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.1982.tb01674.x"},{"key":"e_1_2_7_4_1","first-page":"151","article-title":"Puppy production to the weaning age","volume":"130","author":"Andersen A. 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