{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T04:01:53Z","timestamp":1792814513588,"version":"4.5.0"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,5,21]],"date-time":"2018-05-21T00:00:00Z","timestamp":1526860800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present investigation was carried out to study the effect of various levels of dissolved oxygen (DO) on reactive oxygen species (ROS) and cryocapacitation\u2010like changes in bull sperm. Egg yolk\u2013Tris\u2013glycerol (EYTG) extender was split into four subextenders; viz., Extender I (control; no flushing with liquid nitrogen (LN<jats:sub>2<\/jats:sub>)), Extender II, Extender III and Extender IV were flushed with LN<jats:sub>2<\/jats:sub> for 40, 16 and 8\u00a0min, respectively. The DO levels were standardized to 11.7, 2, 4 and 8\u00a0ppm, respectively, in control (Extender I), Extender II, Extender III and Extender IV. Ejaculates with mass motility of\u00a0\u2265\u00a03+ were divided into group I (diluted with Extender I), group II (diluted with Extender II), group III (diluted with Extender III) and group IV (diluted with Extender IV) up to 80\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup>\u00a0sperm\/ml. Extended semen samples were packed in French mini straws (0.25\u00a0ml), equilibrated and cryopreserved. Semen samples were evaluated at prefreeze and post\u2010thaw stage for various parameters (DO, progressive motility (PM), viability (VIB), acrosomal integrity (AI), hypo\u2010osmotic swelling (HOS) test, ROS, cholesterol (C) and phospholipid (P). The percentage of PM, VIB, AI, HOS test, cholesterol (C) and phospholipid (P) levels, and capacitated sperm were significantly (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) higher in groups III and IV as compared to groups I and II. However, the acrosome\u2010reacted sperm (%; pattern AR) were significantly (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) decreased in group III as compared to all other groups. Besides the proportion of sperm displaying tyrosine\u2010phosphorylated pattern, EA (fluorescence at both equatorial and anterior acrosomal regions, i.e. high capacitation level) was significantly (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) reduced in group III compared to all other groups. In conclusion, varying DO levels in the extender significantly affect sperm quality, ROS production and capacitation\u2010like changes in bulls.<\/jats:p>","DOI":"10.1111\/rda.13200","type":"journal-article","created":{"date-parts":[[2018,5,21]],"date-time":"2018-05-21T14:35:15Z","timestamp":1526913315000},"page":"1033-1040","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Effect of various levels of dissolved oxygen on reactive oxygen species and cryocapacitation\u2010like changes in bull sperm"],"prefix":"10.1111","volume":"53","author":[{"given":"Beigh Y.","family":"Amin","sequence":"first","affiliation":[{"name":"Division of Animal Reproduction ICAR\u2010Indian Veterinary Research Institute Izatnagar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jai K.","family":"Prasad","sequence":"additional","affiliation":[{"name":"Division of Animal Reproduction ICAR\u2010Indian Veterinary Research Institute Izatnagar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subrata K.","family":"Ghosh","sequence":"additional","affiliation":[{"name":"Division of Animal Reproduction ICAR\u2010Indian Veterinary Research Institute Izatnagar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8534-8828","authenticated-orcid":false,"given":"Shabir A.","family":"Lone","sequence":"additional","affiliation":[{"name":"Artificial Breeding Research Center (ABRC), Animal Reproduction, Gynecology and Obstetrics ICAR\u2010National Dairy Research Institute Karnal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhishek","family":"Kumar","sequence":"additional","affiliation":[{"name":"Division of Animal Reproduction ICAR\u2010Indian Veterinary Research Institute Izatnagar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul R.","family":"Mustapha","sequence":"additional","affiliation":[{"name":"Department of Theriogenology, Faculty of Veterinary Medicine University of Maiduguri Borno State Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omer","family":"Din","sequence":"additional","affiliation":[{"name":"Division of Animal Reproduction ICAR\u2010Indian Veterinary Research Institute Izatnagar India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajay","family":"Kumar","sequence":"additional","affiliation":[{"name":"Biochemistry Division ICAR\u2010Indian Veterinary Research Institute Izatnagar India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,5,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2605.2005.00630.x"},{"key":"e_1_2_8_3_1","first-page":"717","volume-title":"Equine reproduction","author":"Amann R. 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