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We examined the relative expression level of seven important genes related to ERK\/MAPK pathway and DNMTs (<jats:italic>DNMT1<\/jats:italic>, <jats:italic>DNMT3a<\/jats:italic> and <jats:italic>DNMT3b<\/jats:italic>) by quantitative real\u2010time PCR in buffalo blastocysts produced by Hand\u2010made cloning and compared it with that in blastocyst\u2010stage embryos produced by in vitro fertilization (IVF). The expression level of six of seven genes related to ERK\/MAPK pathway examined i.e., <jats:italic>p21RAS, RAF1, AKT1, ERK2, PIK3R2 <\/jats:italic>and c\u2010<jats:italic>Myc<\/jats:italic> was significantly higher (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) in cloned than in IVF embryos. However, the expression level of <jats:italic>FOS<\/jats:italic> was lower (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.005) in cloned than in IVF embryos. The relative expression level of <jats:italic>DNMT3a<\/jats:italic> and <jats:italic>DNMT3b<\/jats:italic> but not that of <jats:italic>DNMT1<\/jats:italic> was significantly higher (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) in cloned than in IVF embryos. These results indicate that the cloned embryos exhibit an abnormal expression of several important genes related to ERK\/MAPK pathway and DNMTs. Although a direct link between ERK\/MAPK pathway and DNMTs was not examined in the present study, it can be speculated that ERK\/MAPK pathway may have a role in regulating the expression of DNMTs in embryos, as also observed in other tissues.<\/jats:p>","DOI":"10.1111\/rda.13217","type":"journal-article","created":{"date-parts":[[2018,7,27]],"date-time":"2018-07-27T08:24:13Z","timestamp":1532679853000},"page":"1247-1252","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Buffalo SCNT embryos exhibit abnormal gene expression of ERK\/MAPK pathway and DNA methylation"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2310-1384","authenticated-orcid":false,"given":"Disha","family":"Ashok","sequence":"first","affiliation":[{"name":"Embryo Biotechnology Laboratory, Animal Biotechnology Centre National Dairy Research Institute Karnal  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