{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T12:16:12Z","timestamp":1773663372753,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,7,2]],"date-time":"2018-07-02T00:00:00Z","timestamp":1530489600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["15\u201004\u201003258"],"award-info":[{"award-number":["15\u201004\u201003258"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Far\u2010Eastern wildcat (<jats:italic>Prionailurus bengalensis euptilurus<\/jats:italic>) is a rare and poorly investigated nondomestic felid species. An attempt of freezing and cryopreserving Far\u2010Eastern wildcat spermatozoa in CaniPlus Freeze (CPF) medium is reported. Sperm was collected by electroejaculation from five adult Far\u2010Eastern wildcat captive\u2010born males. Epididymal spermatozoa from five adult randomly bred domestic cat males were used as a reference. The viability of frozen\u2013thawed spermatozoa evaluated by double staining with SYBR Green I and PI followed by the subsequent confocal laser scanning microscopy (CLSM) was 38.2%\u00a0\u00b1\u00a03.0% for the domestic cat and 38.0%\u00a0\u00b1\u00a010.2% for the Far\u2010Eastern wildcat. The motility of frozen\u2013thawed spermatozoa was 30.8%\u00a0\u00b1\u00a09.8% for the domestic cat and 33.7%\u00a0\u00b1\u00a015.1% for the Far\u2010Eastern wildcat. Sperm morphology was assessed by light microscopy. The total percentage of normal spermatozoa after freezing and thawing was 51.9\u00a0\u00b1\u00a05.9 for the domestic cat and 55.0%\u00a0\u00b1\u00a06.4% for the Far\u2010Eastern wildcat. Defects of flagella were the most frequently observed abnormalities in both species (32.2%\u00a0\u00b1\u00a04.8% and 30.8%\u00a0\u00b1\u00a04.4% of all reported anomalies for the domestic cat and Far\u2010Eastern wildcat, respectively). Domestic cat epididymal and Far\u2010Eastern ejaculatory spermatozoa fertilized in vitro\u2010matured oocytes of the domestic cat (30.0%\u00a0\u00b1\u00a05.5% and 35.5%\u00a0\u00b1\u00a015.0%, respectively). Taken together, these results suggest that the freezing of Far\u2010Eastern wildcat spermatozoa with CPF medium is a suitable method for Felidae cryopreservation.<\/jats:p>","DOI":"10.1111\/rda.13230","type":"journal-article","created":{"date-parts":[[2018,7,2]],"date-time":"2018-07-02T08:52:16Z","timestamp":1530521536000},"page":"1219-1226","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Sperm cryopreservation in the Far\u2010Eastern wildcat (<i>Prionailurus bengalensis euptilurus<\/i>)"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7119-0356","authenticated-orcid":false,"given":"Sergei","family":"Amstislavsky","sequence":"first","affiliation":[{"name":"Institute of Cytology and Genetics Russian Academy of Sciences, Siberian Branch Novosibirsk Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugeny","family":"Brusentsev","sequence":"additional","affiliation":[{"name":"Institute of Cytology and Genetics Russian Academy of Sciences, Siberian Branch Novosibirsk Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Kizilova","sequence":"additional","affiliation":[{"name":"Institute of Cytology and Genetics Russian Academy of Sciences, Siberian Branch Novosibirsk Russia"},{"name":"Novosibirsk State University Novosibirsk Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Mokrousova","sequence":"additional","affiliation":[{"name":"Institute of Cytology and Genetics Russian Academy of Sciences, Siberian Branch Novosibirsk Russia"},{"name":"Novosibirsk State University Novosibirsk Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeria","family":"Kozhevnikova","sequence":"additional","affiliation":[{"name":"Institute of Cytology and Genetics Russian Academy of Sciences, Siberian Branch Novosibirsk Russia"},{"name":"Novosibirsk State University Novosibirsk Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatyana","family":"Abramova","sequence":"additional","affiliation":[{"name":"Institute of Cytology and Genetics Russian Academy of Sciences, Siberian Branch Novosibirsk Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina","family":"Rozhkova","sequence":"additional","affiliation":[{"name":"Institute of Cytology and Genetics Russian Academy of Sciences, Siberian Branch Novosibirsk Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergey","family":"Naidenko","sequence":"additional","affiliation":[{"name":"A.N.Severtsov Institute of Ecology and Evolution Russian Academy of Sciences Moscow Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,7,2]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"604","article-title":"Cryopreservation of epididymal semen in djungarian (Phodopus sungorus) and Campbell\u2019s (Phodopus campbelli) hamsters","volume":"95","author":"Amstislavsky S. 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Reprod Domest Anim 44","volume":"2","author":"Chatdarong K.","year":"2009","journal-title":"Suppl"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1994.tb00510.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.05.015"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.02.036"},{"key":"e_1_2_8_11_1","first-page":"245","article-title":"Establishment of cryopreservation of leopard cat semen collected by electro\u2010ejaculation method","volume":"26","author":"Ha A. 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