{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T15:50:22Z","timestamp":1780415422292,"version":"3.54.1"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,7,9]],"date-time":"2018-07-09T00:00:00Z","timestamp":1531094400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31470077"],"award-info":[{"award-number":["31470077"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to investigate the effects of different concentrations of 1,2\u2010bis\u2010(o\u2010aminophenoxy)\u2010ethane\u2010N,N,N0\u00a0N0\u2010tetraacetic acid, tetra\u2010acetoxymethyl ester (BAPTA\u2010AM), an intracellular calcium chelating agent, on stallion semen cooling and freezing\u2013thawing. After collection, semen was extended (1:1 v\/v) on a skim milk\u2010based extender, centrifuged and resuspended at 400\u00a0million\/ml into cooling or freezing extenders containing 0, 5, 25, 50, 100 and 200\u00a0\u03bc\u039c BAPTA\u2010AM. Motility parameters were assessed after cooling in Equitainer at 5\u00b0C for 12, 24, 48, 72 and 120\u00a0hr and after freezing\u2013thawing. In addition, mitochondrial membrane potential, intracellular ATP, reactive oxygen species and malondialdehyde concentrations were measured in cryopreserved\u2010thawed semen. Cooled stored (48\u00a0hr) semen containing 50\u00a0\u03bc\u039c BAPTA\u2010AM and control extender (0\u00a0\u03bc\u039c BAPTA\u2010AM) was used to assess fertility. Inclusion of 50\u00a0\u03bc\u039c BAPTA\u2010AM resulted in superior sperm motility parameters during cooled storage when compared to other groups (<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05). Furthermore, semen cryopreserved in extender containing 50\u00a0\u03bc\u039c BAPTA\u2010AM showed increased intracellular ATP and mitochondrial membrane potential, whereas reactive oxygen species and malondialdehyde were increased after thawing for all groups (<jats:italic>p\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05). Addition of 50\u00a0\u03bc\u039c BAPTA\u2010AM to cooling extender resulted in similar pregnancy rates to the control group (75% vs. 73.6%, respectively;<jats:italic>p\u00a0<\/jats:italic>&gt;<jats:italic>\u00a0<\/jats:italic>0.05). In conclusion, the addition of BAPTA\u2010AM to semen extenders aided stallion semen cryopreservation in a dose\u2010dependent manner. Furthermore, the cooling extender supplemented with 50\u00a0\u03bc\u039c BAPTA\u2010AM could be used to prolong the sperm motility during cooling without apparently compromising fertility. Field trials should be conducted to assess fertility of cryopreserved stallion semen with BAPTA\u2010AM.<\/jats:p>","DOI":"10.1111\/rda.13245","type":"journal-article","created":{"date-parts":[[2018,7,9]],"date-time":"2018-07-09T10:27:17Z","timestamp":1531132037000},"page":"1235-1242","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Intracellular calcium chelating agent (BAPTA\u2010AM) aids stallion semen cooling and freezing\u2013thawing"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4284-5717","authenticated-orcid":false,"given":"Shuaishuai","family":"Wu","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology China Agricultural University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor F.","family":"Canisso","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Medicine, College of Veterinary Medicine University of Illinois Urbana\u2010Champaign Urbana Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weigang","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology China Agricultural University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ihteshamu","family":"Ul Haq","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology and Genetic Engineering University of Agriculture Peshawar Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology China Agricultural University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Han","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology China Agricultural University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenming","family":"Zeng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology China Agricultural University Beijing China"},{"name":"College of Animal Science and Technology Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0306892101"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/cryo.1995.1025"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ctep.2007.09.003"},{"key":"e_1_2_8_5_1","volume-title":"Principles of enzymatic analysis","author":"Bergmeyer H. 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