{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:08:53Z","timestamp":1787162933074,"version":"build-2736575974"},"reference-count":61,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,7,16]],"date-time":"2018-07-16T00:00:00Z","timestamp":1531699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2018,12]]},"abstract":"Contents<\/jats:title>\n \n The objective of this study was to examine the different concentrations of antipain and trehalose combination on post\u2010thawed quality of ram semen cryopreserved in tris extender. Ejaculates were collected from four rams using the artificial vagina, pooled at 37\u00b0C and diluted with (A\n 0<\/jats:sub>\n \u00a0Tre\n 0<\/jats:sub>\n : antipain 0\u00a0\u03bcM and trehalose 0\u00a0\n mM<\/jats:styled-content>\n (Control); A\n 10<\/jats:sub>\n \u00a0Tre\n 0<\/jats:sub>\n ; A\n 50<\/jats:sub>\n \u00a0Tre\n 0<\/jats:sub>\n ; A\n 0<\/jats:sub>\n \u00a0Tre\n 30<\/jats:sub>\n ; A\n 0<\/jats:sub>\n \u00a0Tre\n 60<\/jats:sub>\n ; A\n 10<\/jats:sub>\n \u00a0Tre\n 60<\/jats:sub>\n ; A\n 10<\/jats:sub>\n \u00a0Tre\n 30<\/jats:sub>\n ; A\n 50<\/jats:sub>\n \u00a0Tre\n 30<\/jats:sub>\n and A\n 50<\/jats:sub>\n \u00a0Tre\n 60<\/jats:sub>\n ). Diluted semen samples were gradually cooled down from 37 to 5\u00b0C in a cold cabinet; then, they were loaded into 0.25\u00a0\n ml<\/jats:styled-content>\n straws, frozen and stored in liquid nitrogen. Sperm motility (\n CASA<\/jats:styled-content>\n ), viability, membrane functionality and abnormality were evaluated after thawing process. Progressive motility in extender supplemented with A\n 10<\/jats:sub>\n \u00a0Tre\n 0<\/jats:sub>\n , A\n 0<\/jats:sub>\n \u00a0Tre\n 30<\/jats:sub>\n and A\n 10<\/jats:sub>\n \u00a0Tre\n 60<\/jats:sub>\n significantly (\n p<\/jats:italic>\n \u00a0<\u00a00.05) higher as compared to the control (A\n 10<\/jats:sub>\n \u00a0Tre\n 0<\/jats:sub>\n ). A\n 10<\/jats:sub>\n \u00a0Tre\n 60<\/jats:sub>\n (47.50\u00a0\u00b1\u00a00.73) provided the best maintenance of progressive motility in comparison with the control (40.50\u00a0\u00b1\u00a00.73). No significant differences were observed between all treated groups in terms of total motility,\n VAP<\/jats:styled-content>\n ,\n VSL<\/jats:styled-content>\n ,\n VCL<\/jats:styled-content>\n ,\n ALH<\/jats:styled-content>\n ,\n BCF<\/jats:styled-content>\n ,\n STR<\/jats:styled-content>\n and\n LIN<\/jats:styled-content>\n . The percentages of sperm with viable were significantly higher in extenders supplemented with A\n 10<\/jats:sub>\n \u00a0Tre\n 0<\/jats:sub>\n , A\n 50<\/jats:sub>\n \u00a0Tre\n 0<\/jats:sub>\n , A\n 0<\/jats:sub>\n \u00a0Tre\n 30<\/jats:sub>\n and A\n 10<\/jats:sub>\n \u00a0Tre\n 60<\/jats:sub>\n , compared to control. Addition of A\n 10<\/jats:sub>\n \u00a0Tre\n 0<\/jats:sub>\n , A\n 50<\/jats:sub>\n \u00a0Tre\n 0<\/jats:sub>\n and A\n 10<\/jats:sub>\n \u00a0Tre\n 60<\/jats:sub>\n to extenders improved the percentages of sperm abnormality, compared to the controls. A\n 10<\/jats:sub>\n \u00a0Tre\n 60<\/jats:sub>\n (67.84\u00a0\u00b1\u00a01.51) treatment provided the best maintenance of normal morphology compared to the other treatments. The supplementation with A\n 10<\/jats:sub>\n \u00a0Tre\n 0<\/jats:sub>\n , A\n 0<\/jats:sub>\n \u00a0Tre\n 60<\/jats:sub>\n and A\n 10<\/jats:sub>\n \u00a0Tre\n 60<\/jats:sub>\n improved the percentage of sperm membrane functionality when compared to the control (\n p<\/jats:italic>\n \u00a0<\u00a00.05). Comparing these results with those of control diluents, the effects of supplementation were better except for A\n 50<\/jats:sub>\n \u00a0Tre\n 60<\/jats:sub>\n group. In conclusion, when combination of antipain (10\u00a0\u03bcM) and trehalose (30 and 60\u00a0\n mM<\/jats:styled-content>\n ) was added, they conferred a great cryosurvival capacity with their synergic effects during freeze\u2010thawing process.\n <\/jats:p>","DOI":"10.1111\/rda.13253","type":"journal-article","created":{"date-parts":[[2018,7,16]],"date-time":"2018-07-16T09:51:01Z","timestamp":1531734661000},"page":"1359-1366","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The protease inhibitor antipain has a beneficial synergistic effect with trehalose for ram semen cryopreservation"],"prefix":"10.1111","volume":"53","author":[{"given":"Bahareh","family":"Akhtarshenas","sequence":"first","affiliation":[{"name":"IVF Laboratory Department of Animal Science Faculty of Agricultural and Engineering Science Razi University Kermanshah Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4810-2415","authenticated-orcid":false,"given":"Hamed","family":"Karami Shabankareh","sequence":"additional","affiliation":[{"name":"IVF Laboratory Department of Animal Science Faculty of Agricultural and Engineering Science Razi University Kermanshah Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hadi","family":"Hajarian","sequence":"additional","affiliation":[{"name":"IVF Laboratory Department of Animal Science Faculty of Agricultural and Engineering Science Razi University Kermanshah Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa Numan","family":"Bucak","sequence":"additional","affiliation":[{"name":"Department of reproduction and artificial insemination Faculty of Veterinary Selcuk University Selcuk Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali Reza","family":"Abdolmohammadi","sequence":"additional","affiliation":[{"name":"IVF Laboratory Department of Animal Science Faculty of Agricultural and Engineering Science Razi University Kermanshah Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mojtaba","family":"Dashtizad","sequence":"additional","affiliation":[{"name":"Department of Animal Science National Institute of genetics and biotechnology Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(199703)46:3<370::AID-MRD16>3.0.CO;2-2"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(02)00653-2"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2005.02.002"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2008.03.002"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/j.1939-4640.2000.tb03268.x","article-title":"Semen cryopreservation in domestic animals: A damaging and capacitating phenomenon minireview","volume":"21","author":"Bailey J. 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